JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB197476

Mouse Interleukin Antibody Array (10 Targets) - Quantitative

Be the first to review this product! Submit a review

|

(0 Publication)

ab197476 can be used for quantitative measurement of 10 Mouse interleukines.

View Alternative Names

Il28a, Ifnl2, Interferon lambda-2, IFN-lambda-2, Interleukin-28A, IL-28A

3 Images
Multiplex Protein Detection - Mouse Interleukin Antibody Array (10 Targets) - Quantitative (AB197476)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Mouse Interleukin Antibody Array (10 Targets) - Quantitative (AB197476)

Standard Curve obtained with Mouse Interleukin Antibody Array (10 Targets) - Quantitative (ab197476)

Multiplex Protein Detection - Mouse Interleukin Antibody Array (10 Targets) - Quantitative (AB197476)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Mouse Interleukin Antibody Array (10 Targets) - Quantitative (AB197476)

Mouse Interleukin Antibody Array (10 Targets) - Quantitative (ab197476) Assay Summary

Multiplex Protein Detection - Mouse Interleukin Antibody Array (10 Targets) - Quantitative (AB197476)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Mouse Interleukin Antibody Array (10 Targets) - Quantitative (AB197476)

Array Map for Mouse Interleukin Antibody Array (10 Targets) - Quantitative (ab197476)

Key facts

Assay type

Quantitative

Reacts with

Mouse

Target

Ifnl2

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

style
left-column

Product details

ab197476 can be used for quantitative measurement of 10 Mouse interleukines. Suitable for serum, plasma, cell culture media, other body fluid, cell and tissue lysates.
Targets names: IL-1ra, IL-2Rα, IL-6R, IL-11, IL-12p40, IL-17B, IL-17E, IL-17F, IL-20, IL-28.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. "

style
left-column

What's included?

{ "values": { "8Test": { "sellingSize": "8 Test", "publicAssetCode":"ab197476-8Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Adhesive Film", "number":"AB197476-CMP09" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197476-CMP07" }, { "size":"1 x 1 Vial", "name":"Mouse Interleukin Array (10 T) Lyophilized Cytokine Standard Mix", "number":"AB197476-CMP05" }, { "size":"1 x 15 mL", "name":"Sample Diluent", "number":"AB197476-CMP01" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197476-CMP06" }, { "size":"1 x 1 Slide", "name":"Mouse Interleukin Array (10 T) Glass Slide", "number":"AB197476-CMP04" }, { "size":"1 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197476-CMP02" }, { "size":"1 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197476-CMP08" }, { "size":"1 x 25 µL", "name":"Mouse Interleukin Array (10 T) Biotinylated Antibody Cocktail", "number":"AB197476-CMP03" } ] }, "22Test": { "sellingSize": "22 Test", "publicAssetCode":"ab197476-22Test", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Adhesive Film", "number":"AB197476-CMP09" }, { "size":"2 x 25 µL", "name":"Mouse Interleukin Array (10 T) Biotinylated Antibody Cocktail", "number":"AB197476-CMP03" }, { "size":"1 x 1 Vial", "name":"Mouse Interleukin Array (10 T) Lyophilized Cytokine Standard Mix", "number":"AB197476-CMP05" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197476-CMP07" }, { "size":"1 x 15 mL", "name":"Sample Diluent", "number":"AB197476-CMP01" }, { "size":"3 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197476-CMP06" }, { "size":"2 x 1 Slide", "name":"Mouse Interleukin Array (10 T) Glass Slide", "number":"AB197476-CMP04" }, { "size":"2 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197476-CMP08" }, { "size":"1 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197476-CMP02" } ] }, "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab197476-50Test", "assetComponentDetails": [ { "size":"4 x 1 Unit", "name":"Adhesive Film", "number":"AB197476-CMP09", "productcode":"" }, { "size":"6 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197476-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Interleukin Array (10 T) Lyophilized Cytokine Standard Mix", "number":"AB197476-CMP05", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197476-CMP07", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197476-CMP02", "productcode":"" }, { "size":"4 x 25 µL", "name":"Mouse Interleukin Array (10 T) Biotinylated Antibody Cocktail", "number":"AB197476-CMP03", "productcode":"" }, { "size":"4 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197476-CMP08", "productcode":"" }, { "size":"2 x 15 mL", "name":"Sample Diluent", "number":"AB197476-CMP01", "productcode":"" }, { "size":"4 x 1 Slide", "name":"Mouse Interleukin Array (10 T) Glass Slide", "number":"AB197476-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information Ifnl2

Additional targets

Il2ra,Il17b,Il2ra,Il17b,Il11,Il12b,Il17f,Il1rn,Il20,IL-25,Il6ra
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Multiplex Kits

AB197475

Mouse Interleukin Antibody Array (20 Targets) - Quantitative

Multiplex Protein Detection - Mouse Interleukin Antibody Array (20 Targets) - Quantitative (AB197475)

  • 0
  • 0
Multiplex Kits

AB197474

Mouse Interleukin Antibody Array (30 Targets) - Quantitative

  • 0
  • 0
Multiplex Kits

AB197465

Mouse Cytokine Antibody Array A (20 Targets) - Quantitative

Multiplex Protein Detection - Mouse Cytokine Antibody Array A (20 Targets) - Quantitative (AB197465)

  • 0
  • 0
Multiplex Kits

AB193659

Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 96 Targets)

Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 96 Targets) (AB193659)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com