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AB197483

Rat Cytokine Antibody Array (27 Targets) - Quantitative

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ab197483 can be used for quantitative measurement of 27 Rat cytokines.

View Alternative Names

Rage, Ager, Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1, CD62L, Lnhr, Ly-22, Sell, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, LAM-1, LECAM1, CD54, Intercellular adhesion molecule 1, ICAM-1, RICAM-I, Icam1, Scyb5, Cxcl5, C-X-C motif chemokine 5, Cytokine LIX, Small-inducible cytokine B5, Interferon gamma, IFN-gamma, Ifng, Prolactin receptor, PRL-R, Lactogen receptor, Prlr, Il-4, Il4, Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, Interleukin-1 alpha, IL-1 alpha, Il1a, Cinc1, Gro, Scyb1, Cxcl1, Growth-regulated alpha protein, C-X-C motif chemokine 1, Cytokine-induced neutrophil chemoattractant 1, Platelet-derived growth factor-inducible protein KC, CINC-1, Il-6, Il6, Interleukin-6, IL-6, Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Il-10, Il10, Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, Vegf, Vegfa, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, Interleukin-1 beta, IL-1 beta, Il1b, Csfgm, Csf2, Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF, Il-13, Il13, Interleukin-13, IL-13, T-cell activation protein P600, Ciliary neurotrophic factor, CNTF, Cntf, Il-2, Il2, Interleukin-2, IL-2, T-cell growth factor, TCGF, Rpa1, Pdgfa, Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, Acc1, Fkn, Scyd1, Cx3cl1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, Cinc3, Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, Macrophage inflammatory protein 2, CINC-3, MIP2, Timp-1, Timp1, Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases 1, TIMP-1, Cinc2, Cxcl3, C-X-C motif chemokine 3, Cytokine-induced neutrophil chemoattractant 2, Macrophage inflammatory protein 2-alpha/beta, CINC-2, MIP2-alpha/beta, Ngfb, Ngf, Beta-nerve growth factor, Beta-NGF, Beta thromboglobulin, Beta-TG, C-X-C motif chemokine 7, CTAP 3, CTAP-III, CTAP-III(1-81), CXC chemokine ligand 7, CXCL7_HUMAN, Chemokine (C X C motif) ligand 7, Connective tissue activating peptide III, LA-PF4, LDGF, Leukocyte-derived growth factor, Low-affinity platelet factor IV, MDGF, Macrophage-derived growth factor, NAP-2, NAP-2(1-63), NAP-2(1-66), NAP-2(73), NAP-2(74), Neutrophil activating peptide 2, Neutrophil-activating peptide 2(1-63), PBP, PPBP, Platelet basic protein, Pro platelet basic protein, Pro platelet basic protein (chemokine (C-X-C motif) ligand 7), SCYB7, Small inducible cytokine subfamily B member 7, Small-inducible cytokine B7, TC2, TGB, TGB1, THBGB, THBGB1, Thrombocidin 1, Thrombocidin 2, Thromboglobulin, beta-1

3 Images
Multiplex Protein Detection - Rat Cytokine Antibody Array (27 Targets) - Quantitative (AB197483)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Rat Cytokine Antibody Array (27 Targets) - Quantitative (AB197483)

Standard Curve obtained with Rat Cytokine Antibody Array (27 Targets) - Quantitative (ab197483).

Multiplex Protein Detection - Rat Cytokine Antibody Array (27 Targets) - Quantitative (AB197483)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Rat Cytokine Antibody Array (27 Targets) - Quantitative (AB197483)

Array Map for Rat Cytokine Antibody Array (27 Targets) - Quantitative (ab197483).

Multiplex Protein Detection - Rat Cytokine Antibody Array (27 Targets) - Quantitative (AB197483)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Rat Cytokine Antibody Array (27 Targets) - Quantitative (AB197483)

Rat Cytokine Antibody Array (27 Targets) - Quantitative (ab197483) Assay Summary

Key facts

Reacts with

Rat

Target

Ager

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

Product details

ab197483 can be used for quantitative measurement of 27 Rat cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.

Targets names: B7-2, β-NGF, CINC-1, CINC-2, CINC-3, CNTF, Fractalkine, GM-CSF, ICAM-1, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-13, LIX, L-Selectin, MCP-1, PDGF-AA, Prolactin R, RAGE, TCK-1, TIMP-1, TNFα, VEGF.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. "

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Cell surface pattern recognition receptor that senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, histones, phospholipids and glycosaminoglycans (PubMed : 19910580, PubMed : 28627626). Advanced glycosylation end products are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. These ligands accumulate at inflammatory sites during the pathogenesis of various diseases including diabetes, vascular complications, neurodegenerative disorders and cancers, and RAGE transduces their binding into pro-inflammatory responses. Upon ligand binding, uses TIRAP and MYD88 as adapters to transduce the signal ultimately leading to the induction of inflammatory cytokines IL6, IL8 and TNFalpha through activation of NF-kappa-B. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key pro-inflammatory mediators (By similarity). Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Participates in endothelial albumin transcytosis together with HMGB1 through the RAGE/SRC/Caveolin-1 pathway, leading to endothelial hyperpermeability. Mediates the loading of HMGB1 in extracellular vesicles (EVs) that shuttle HMGB1 to hepatocytes by transferrin-mediated endocytosis and subsequently promote hepatocyte pyroptosis by activating the NLRP3 inflammasome. Binds to DNA and promotes extracellular hypomethylated DNA (CpG DNA) uptake by cells via the endosomal route to activate inflammatory responses (By similarity). Mediates phagocytosis by non-professional phagocytes (NPP) and this is enhanced by binding to ligands including RNA, DNA, HMGB1 and histones (By similarity). Promotes NPP-mediated phagocytosis of Saccharomyces cerevisiae spores by binding to RNA attached to the spore wall (By similarity). Also promotes NPP-mediated phagocytosis of apoptotic cells (By similarity). Following DNA damage, recruited to DNA double-strand break sites where it colocalizes with the MRN repair complex via interaction with double-strand break repair protein MRE11 (By similarity). Enhances the endonuclease activity of MRE11, promoting the end resection of damaged DNA (By similarity). Promotes DNA damage repair in trophoblasts which enhances trophoblast invasion and contributes to placental development and maintenance (By similarity). Protects cells from DNA replication stress by localizing to damaged replication forks where it stabilizes the MCM2-7 complex and promotes faithful progression of the replication fork (By similarity).
See full target information Ager

Additional targets

Sell,Cd86,Cxcl1,Cxcl2,Cxcl3,Cxcl5,Icam1,Ifng,Il10,Il13,Il1a,Il1b,Cx3cl1,Il2,Il4,Il6,Ngf,CXCL7/PBP,Prlr,Timp1,Tnf,Vegfa,Pdgfa,Ccl2,Cntf,Csf2
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websiteProtocolBooklet
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