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AB197482

Rat Cytokine Antibody Array B (10 Targets) - Quantitative

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ab197482 can be used for quantitative measurement of 10 Rat cytokines.

View Alternative Names

Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1

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Multiplex Protein Detection - Rat Cytokine Antibody Array B (10 Targets) - Quantitative (AB197482)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Rat Cytokine Antibody Array B (10 Targets) - Quantitative (AB197482)

Standard Curve obtained with Rat Cytokine Antibody Array B (10 Targets) - Quantitative (ab197482)

Multiplex Protein Detection - Rat Cytokine Antibody Array B (10 Targets) - Quantitative (AB197482)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Rat Cytokine Antibody Array B (10 Targets) - Quantitative (AB197482)

Array Map for Rat Cytokine Antibody Array B (10 Targets) - Quantitative (ab197482)

Multiplex Protein Detection - Rat Cytokine Antibody Array B (10 Targets) - Quantitative (AB197482)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Rat Cytokine Antibody Array B (10 Targets) - Quantitative (AB197482)

Rat Cytokine Antibody Array B (10 Targets) - Quantitative (ab197482) Assay Summary

Key facts

Assay type

Quantitative

Reacts with

Rat

Target

Ccl2

target

Detection method

Fluorescent

Assay Platform

Multiplex

Sample types

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

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Product details

ab197482 can be used for quantitative measurement of 10 Rat cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets names: ICAM-1, IFNγ, IL-1β, IL-6, IL-10, Leptin, L-selectin, MCP-1, TIMP-1, TNFα.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. "

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity).
See full target information Ccl2

Additional targets

Sell,Icam1,Ifng,Il10,Il1b,Il6,Lep,Timp1,Tnf
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Product promise

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