Alpha Synuclein Antibody Sampler Panel (ab263465) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Key facts
- Reactive species
- Mouse, Rat, Human
- Target
- SNCA
(See target data below)
Associated Products
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Target data
Function
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Additional Targets
SNCA
Alternative names
NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor
What's included?
Recommended products
Alpha Synuclein Antibody Sampler Panel (ab263465) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Reactive species
- Mouse, Rat, Human
- Target
- SNCA
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Alpha Synuclein Antibody Sampler Panel ab263465 contains multiple trial-sized versions of anti-human, mouse, and rat antibody clones against Alpha Synuclein, specifically selected for high performance in various applications. This panel contains 4 recombinant rabbit monoclonal antibodies and 2 mouse monoclonal antibodies against Alpha Synuclein. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EP1536Y] to Alpha-synuclein (phospho S129) (20 μL) ab51253
- Rabbit monoclonal [MJFR1] to Alpha-synuclein (20 μL) ab138501
- Rabbit monoclonal [MJFR-14-6-4-2] to Alpha-synuclein filament - Conformation-Specific (20 μL) ab209538
- Mouse monoclonal [LB 509] to Alpha-synuclein (20 μg) ab27766
- Mouse monoclonal [syn211] to Alpha-synuclein (20 μg) ab80627
- Rabbit monoclonal [EPR20535] to Alpha-synuclein (20 μL) ab212184
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-synuclein often referred to as SNCA or NACP is a protein that plays important mechanical roles in the nervous system. It possesses a mass of approximately 14 kDa and is known for its expression predominately in the brain especially within the presynaptic terminals of neurons. Its primary function involves binding to phospholipids and regulating synaptic vesicle trafficking affecting the release of neurotransmitters. Alpha-synuclein can also adopt multiple conformations including monomeric and aggregated forms which can affect its functional capabilities.
- Biological function summary
Alpha-synuclein impacts several cellular processes including neuronal function and plasticity. It can form aggregates known as Lewy bodies which are distinctive in certain neurodegenerative diseases. Alpha-synuclein is associated with the regulation of dopamine release and uptake affecting synaptic activity and neural communication. Within cells it does not necessarily function as part of a larger protein complex but interacts dynamically with cellular membranes and other synaptic proteins impacting cellular homeostasis and stress response.
- Pathways
This protein integrates into key neuronal processes such as the synaptic vesicle cycle and the autophagy-lysosome pathway. It affects interactions with other proteins like PARK7 (DJ-1) and LRRK2 which influence neurodegenerative processes. Alpha-synuclein’s behavior in protein folding and degradation pathways hints at its role in maintaining protein homeostasis under normal and pathological conditions. Its involvement in the autophagy pathway connects its function to cellular clearing mechanisms and responses to cellular stress.
- Associated diseases and disorders
Alpha-synuclein is linked prominently to Parkinson's disease and dementia with Lewy bodies. These conditions feature the pathological aggregation of alpha-synuclein into insoluble fibrils. Aberrant interactions with proteins such as tau and ubiquitin may exacerbate pathological conditions and contribute to disease progression. These aggregates disrupt cellular functions leading to neuron loss and impaired neural networks highlighting alpha-synuclein as a significant focus in neurodegenerative disease research.
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alpha Synuclein Antibody Sampler Panel (ab263465)
IHC image of alpha Synuclein staining in normal Human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-Alpha-synuclein antibody [MJFR1] ab138501, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Alpha Synuclein Antibody Sampler Panel (ab263465)
Immunocytochemistry/Immunofluorescence analysis of U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling alpha Synuclein with purified Anti-Alpha-synuclein antibody [MJFR1] ab138501 at 1/150 dilution (left panel). Cells were fixed with 4% paraformaldehyde. A Goat anti rabbit IgG(Alexa Fluor®555) (1/200) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain (right panel).
Immunocytochemistry/ Immunofluorescence - Alpha Synuclein Antibody Sampler Panel (ab263465)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized ReNcell VM (Human neural progenitor) cells labeling Alpha-synuclein filament with Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab209538 at 1/5000 dilution, followed by Alexa Fluor® 647 Donkey anti-Rabbit IgG (H+L) secondary antibody at 1/400 dilution (red).
Upper panel counterstain: Anti-aggregated alpha-synuclein antibody clone 5G4 at 1/400 dilution, followed by AlexaFluor®488 secondary detection (green).
Lower panel counterstain: Anti-alpha/beta-synculein antibody at 1/200 dilution, followed by Alexa Fluor® 488 secondary detection (green).
Blocking buffer: 3% bovine serum albumin and 2% fetal bovine serum.
ReNcell VM cells were differentiated in media containing cAMP and GDNF (without bFGF or EGF) and transduced with Ad5C01 viral vector encoding human WT alpha-synuclein filament.
Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab209538 detects alpha-synuclein filaments, which are also recognized by anti-aggregated alpha-synuclein antibody clone 5G4 (upper panel).
Anti-alpha/beta-synculein antibody interacts with total alpha-synuclein in neurons, whereas Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific ab209538 only recognizes the filamentous form (lower panel).
Images were reproduced courtesy of Charles River.
Image courtesy of anonymous customer reviewerImmunocytochemistry/ Immunofluorescence - Alpha Synuclein Antibody Sampler Panel (ab263465)
Human neuroblastoma cells stained for alpha-synuclein (green) using Anti-Alpha-synuclein antibody [LB 509] ab27766 in immunofluorescence. The neuroblastoma cells were fixed with paraformaldehyde and incubated with Anti-Alpha-synuclein antibody [LB 509] ab27766 (used at 5 μg/ml) for 12 hours at 4°C. A FITC conjugated Goat anti-Mouse IgG secondary antibody was then used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alpha Synuclein Antibody Sampler Panel (ab263465)
Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Alpha-synuclein with Anti-Alpha-synuclein antibody [EPR20535] ab212184 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.Cytoplasmic staining on human glioma [PMID: 22112368].Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alpha Synuclein Antibody Sampler Panel (ab263465)
IHC image of alpha Synuclein (phospho S129) staining in Human Parkinson Substantia Nigra formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] ab51253, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.
This image was taken from a customer review submitted by an anonymous reviewer.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alpha Synuclein Antibody Sampler Panel (ab263465)
IHC-P image of Alpha Synuclein (Anti-Alpha-synuclein antibody [LB 509] ab27766) staining in human brain samples from patients with Multiple Systems Atrophy (MSA). The sections were subjected to heat-mediated antigen retrieval with citrate buffer. In addition, some slides received a 15 minute pre-treatment with Formic Acid. Sections were incubated in 20% serum for 30 minutes at +18°C to block non-specific protein-protein interactions. The sections were then incubated with Anti-Alpha-synuclein antibody [LB 509] ab27766 (1:400) for one hour at +18°C, followed by Biotin conjugated anti-mouse goat secondary antibody (1/200). Formic acid pre-treatment (15min) revealed more inclusions in MSA tissue.
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