Astrocyte Marker (ALDH1L1, EAAT1, EAAT2, GFAP) Antibody Sampler Panel

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Astrocyte Marker (ALDH1L1, EAAT1, EAAT2, GFAP) Antibody Sampler Panel (AB226481)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab177463 at 1/50 dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Astrocyte Marker (ALDH1L1, EAAT1, EAAT2, GFAP) Antibody Sampler Panel (AB226481)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse striatum tissue labeling EAAT2 with ab205248 at 1/500 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membrane staining on mouse striatum is observed [PMID : 25391854]. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab150077 at 1/1000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Astrocyte Marker (ALDH1L1, EAAT1, EAAT2, GFAP) Antibody Sampler Panel (AB226481)

ab181036 staining EAAT1 in rat cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Astrocyte Marker (ALDH1L1, EAAT1, EAAT2, GFAP) Antibody Sampler Panel (AB226481)

Immunocytochemistry/Immunofluorescence analysis of mouse cerebellum tissue lysate labelling GFAP with ab68428 at 1/100. The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.2% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Antigen retrieval was performed by heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). Nuclei were counterstained with DAPI (blue). PBS was used instead of the primary antibody as negative control.