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Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel (ab269811) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Images

Immunocytochemistry/ Immunofluorescence - Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel (AB269811), expandable thumbnail
  • Western blot - Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel (AB269811), expandable thumbnail
  • Western blot - Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel (AB269811), expandable thumbnail
  • Western blot - Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel (AB269811), expandable thumbnail
  • Western blot - Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel (AB269811), expandable thumbnail

Key facts

Reactive species

Human, Mouse, Rat

Target

ATG16L1

Reactivity data

Application

ICC/IF

Reactivity

Reacts

Dilution info

-

Notes

Anti-ATG16L1 antibody [EPR15638] - N-terminal ab187671 has not been in house tested for ICC/IF.

Application

IHC-P

Reactivity

Reacts

Dilution info

-

Notes

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker ab109012 has not been in house tested for IHC-P.

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

For optimal WB results using Anti-ATG16L1 (phospho S278) antibody [EPR19016] ab195242, we recommend blocking with 10X Blocking Buffer (10X Blocking Buffer ab126587)

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Target data

Function

Plays an essential role in both canonical and non-canonical autophagy: interacts with ATG12-ATG5 to mediate the lipidation to ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP) (PubMed:23376921, PubMed:23392225, PubMed:24553140, PubMed:24954904, PubMed:27273576, PubMed:29317426, PubMed:30778222, PubMed:33909989). Acts as a molecular hub, coordinating autophagy pathways via distinct domains that support either canonical or non-canonical signaling (PubMed:29317426, PubMed:30778222). During canonical autophagy, interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to ATG8 proteins, to produce a membrane-bound activated form of ATG8 (PubMed:23376921, PubMed:23392225, PubMed:24553140, PubMed:24954904, PubMed:27273576). Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:23376921, PubMed:23392225, PubMed:24553140, PubMed:24954904, PubMed:27273576). As part of the ATG8 conjugation system with ATG5 and ATG12, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity). Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, probably by catalyzing conjugation of phosphatidylserine (PS) to ATG8 (PubMed:33909989). Non-canonical autophagy plays a key role in epithelial cells to limit lethal infection by influenza A (IAV) virus (By similarity). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:22749352, PubMed:25645662). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes an autophagy-dependent antibacterial pathway together with NOD1 or NOD2 (PubMed:20637199). Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966).

Alternative names

Recommended products

Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel (ab269811) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Key facts

Reactive species

Human, Mouse, Rat

Target

ATG16L1

Applications

ICC/IF, IHC-P, WB

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Please note that Recombinant Anti-ATG16L1 (phospho S278) antibody [EPR19016] is for mouse and human reactivity only. The rest of the clones in this panel react with human, mouse, and rat.

Autophagy Analysis (ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin, M6PR) Antibody Sampler Panel ab269811 contains multiple trial-sized versions of anti-human, mouse and rat antibody clones against ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin and M6PR, specifically selected for high performance in in various applications. This panel contains 6 recombinant rabbit monoclonal antibodies against human, mouse and rat ATG16L1, ATG16L1 pS278, SQSTM1, LC3B, Ubiquitin and M6PR. They are provided as a sampler panel to allow you to easily evaluate each antibody.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

Recombinant Anti-ATG16L1 (phospho S278) antibody [EPR19016] (20 μL) ab195242

Recombinant Anti-ATG16L1 antibody [EPR15638] - N-terminal antibody (20 μL) ab187671

Recombinant Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker antibody (20 μL) ab109012

Recombinant Anti-LC3B antibody [EPR18709] - Autophagosome Marker antibody (20 μL) ab192890

Recombinant Anti-Ubiquitin antibody [EPR8830] (20 μL) ab134953

Recombinant Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (20 μL) ab124767

Please note that Recombinant Anti-ATG16L1 (phospho S278) antibody [EPR19016] is for mouse reactivity only. The rest of the clones in this panel react with human, mouse, and rat.

For optimal WB results using ab195242, we recommend blocking with 10X Blocking Buffer (ab126587).


Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ubiquitin acts as a small regulatory protein with a mass of around 8.5 kDa. It is widely expressed in eukaryotic cells and involved in tagging cellular proteins for degradation. M6PR or cation-independent mannose-6-phosphate receptor functions in the lysosomal targeting of proteins aiding in lysosomal enzyme transport. SQSTM1 commonly known as p62 plays an important role in autophagy by linking ubiquitinated proteins to the autophagy machinery. ATG16L1 essential for autophagy participates in the formation of autophagosomes. LC3B a marker of autophagy associates with autophagosomes and its lipidation signifies autophagic activity.

Biological function summary

These proteins serve central roles in cellular homeostasis and degradation processes. Ubiquitin is integral in tagging proteins for proteasomal degradation. M6PR functions within lysosomal enzyme trafficking while p62 aggregates ubiquitinated proteins for autophagic degradation acting as a bridge to autophagic cargo. ATG16L1 stands as part of the autophagy-related gene complex important for the elongation of the autophagosome membrane. LC3B when lipidated embeds into autophagosome membranes distinguishing active autophagy processes.

Pathways

These proteins integrate into the ubiquitin-proteasome and autophagy-lysosome systems. Ubiquitin interacts with various cellular pathways to manage protein turnover and degradation. The recycling of cellular components through autophagy involves p62 and ATG16L1 where LC3B serves as an autophagosome marker. These pathways intersect with cellular stress responses involving other proteins like mTOR and ULK1 which regulate autophagy initiation.

Associated diseases and disorders

These proteins link to neurodegenerative diseases and cancer. Ubiquitin and related pathways play a role in conditions like Parkinson’s disease where impaired protein degradation is a factor. Altered expression of p62 or ATG16L1 can contribute to cancer progression through dysregulated autophagy. Both proteins interact with pathways affecting diseases with aberrant ubiquitin signaling or p62 accumulation associated with pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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