AB228525

Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel

Name: Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (ab228525) | Abcam
Brand: Abcam Limited
SKU: AB228525
Price: 750 USD
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
What is this?

(1 Review)

(3 Publications)

View Alternative Names

APG5L, ASP, ATG5, Autophagy protein 5, APG5-like, Apoptosis-specific protein

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling ATG16L1 with ab187671 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

ab192890 staining LC3B in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells +/- Chloroquine (50μM 24 hours).

The cells were fixed with 100% methanol (5 min) permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab192890 at 1μg/ml and ab195889 Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594) at 1/250 dilution (shown in pseudocolor red) followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor^® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Alexa Fluor^® 488 (ab225383) and Alexa Fluor^® 647 (ab225382) conjugated versions are available for this clone.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

ab108338, at 1/100, staining ATG9A in paraffin-embedded Human colon tissue by Immunohistochemistry.

Lab

Western blot - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : APG5L/ATG5 knockout HAP1 cell lysate (20 μg)
Lane 3 : Raji cell lysate (20 μg)
Lane 4 : Jeg-3 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab108327 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108327 was shown to specifically react with APG5L/ATG5 when APG5L/ATG5 knockout samples were used. Wild-type and APG5L/ATG5 knockout samples were subjected to SDS-PAGE. ab108327 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] (<a href='/en-us/products/primary-antibodies/apg5l-atg5-antibody-epr17552-ab108327'>ab108327</a>)

Predicted band size: 32 kDa

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Lab

Western blot - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : ATG4B knockout HAP1 cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab154843 observed at 47 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab154843 was shown to specifically react with ATG4B when ATG4B knockout samples were used. Wild-type and ATG4B knockout samples were subjected to SDS-PAGE. ab154843 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATG4B antibody [EPR6436(2)] (<a href='/en-us/products/primary-antibodies/atg4b-antibody-epr64362-ab154843'>ab154843</a>)

Predicted band size: 44 kDa

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Key facts

Reacts with

Mouse, Rat, Human

Target

ATG5

target

Applications

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Autophagy Antibody Panel Sampler Panel (ab228525) contains multiple trial-sized versions of clones against APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, and LC3B specifically selected for high performance in various applications. This panel contains 6 recombinant rabbit monoclonal antibodies, 5 of which are knock-out validated. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [EPR1755(2)] to APG5L/ATG5 (20 μL) ab108327

- Rabbit monoclonal [EPR15638] to ATG16L1 - N-terminal (20 μL) ab187671

- Rabbit monoclonal [EPR6436(2)] to ATG4B (20 μL) ab154843

- Rabbit monoclonal [EPR2450(2)] to ATG9A (20 μL) ab108338

- Rabbit monoclonal [EPR19662] to Beclin 1 (20 μL) ab207612

- Rabbit monoclonal [EPR18709] to LC3B (20 μL) ab192890

Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab228525-1Kit", "assetComponentDetails": [ { "size":"2 x 10 µL", "name":"Anti-ATG9A antibody [EPR2450(2)]", "number":"AB228525-CMP06", "productcode":"AB108338" }, { "size":"2 x 10 µL", "name":"Anti-ATG4B antibody [EPR6436(2)]", "number":"AB228525-CMP05", "productcode":"AB154843" }, { "size":"2 x 10 µL", "name":"Anti-APG5L/ATG5 antibody [EPR1755(2)]", "number":"AB228525-CMP01", "productcode":"AB108327" }, { "size":"2 x 10 µL", "name":"Anti-ATG16L1 antibody [EPR15638]", "number":"AB228525-CMP03", "productcode":"AB187671" }, { "size":"2 x 10 µL", "name":"Anti-Beclin 1 antibody [EPR19662]", "number":"AB228525-CMP02", "productcode":"AB207612" }, { "size":"2 x 10 µL", "name":"Anti-LC3B antibody [EPR18709]", "number":"AB228525-CMP04", "productcode":"AB192890" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beclin 1 also known as ATG6 or VPS30 is a central player in the autophagy process. This protein has a molecular mass of approximately 60 kDa and is widely expressed in a variety of tissues. Beclin 1 forms a core component of the class III phosphatidylinositol 3-kinase (PI3K) complex and helps to initiate the formation of pre-autophagosomal structures. Associated proteins like APG5L/ATG5 ATG16L1 ATG9A LC3B and ATG4B also play important roles in the regulation of autophagy. Particularly LC3B serves as an important autophagy marker often used to monitor autophagosomes through autophagy antibodies in experimental settings.

Biological function summary

These proteins coordinate to regulate and execute autophagy a process that cleans up damaged cellular components and helps maintain cellular homeostasis. They assemble into a complex network that orchestrates the formation and maturation of autophagosomes effectively marking them for lysosomal degradation. Beclin 1 interacts with multiple proteins like Atg14 and UVRAG to form different PI3K complexes leading to nucleation of the autophagic membrane. ATG5 ATG12 and ATG16L1 work in a conjugation system that expands the autophagosome membrane while the proteolytic processing of LC3B is essential for autophagosome closure.

Pathways

The autophagy process plays a significant role in cellular pathways like nutrient sensing and stress response. The AMPK/mTOR pathway for example tightly regulates autophagy in response to cellular energy status. AMPK activates autophagy by inhibiting mTOR signaling allowing initiation of autophagosome formation. Proteins such as ATG9A are integral for the trafficking of autophagy-related vesicles affecting the recruitment and movement of membrane source for developing autophagosomes. The balance between PI3K-AKT signaling and mTOR is important for the coordination between cell growth and the autophagic activity mediated by these proteins.

Dysregulated autophagy pathways involving Beclin 1 and associated components like LC3B and ATG5 are linked to cancer and neurodegenerative diseases such as Alzheimer's. Alterations in autophagy can disrupt cellular homeostasis facilitating the development of malignancies through the evasion of cell death. In neurodegenerative contexts impaired autophagy leads to the accumulation of toxic proteins exacerbating disease progression. The interplay between Beclin 1 and proteins like Bcl-2 also links it to apoptosis suggesting that a delicate balance between autophagy and apoptosis is important in preventing these disorders.

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Product protocols

Visit the General protocols
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Target data

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. As part of the ATG8 conjugation system with ATG12 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity).. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.. (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed : 17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established (PubMed : 19666601).

See full target information ATG5

Additional targets

ATG16L1,ATG4B,ATG9A,BECN1,MAP1LC3B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0228723 PubMed38349185

2024

Calpain-2 mediates SARS-CoV-2 entry via regulating ACE2 levels.

Applications

Unspecified application

Species

Unspecified reactive species

Qiru Zeng,Avan Antia,Luis Alberto Casorla-Perez,Maritza Puray-Chavez,Sebla B Kutluay,Matthew A Ciorba,Siyuan Ding

Naunyn-Schmiedeberg's archives of pharmacology 396:1105-1115 PubMed36645429

2023

Doxorubicin-induced acute cardiotoxicity is associated with increased oxidative stress, autophagy, and inflammation in a murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Lorena Dulf,Mihaela Mocan,Camelia Alexandra Coadă,Daniel Vasile Dulf,Remus Moldovan,Ioana Baldea,Anca-Daniela Farcas,Dan Blendea,Adriana Gabriela Filip

Aging 12:21687-21705 PubMed33147167

2020

Dexmedetomidine inhibits inflammatory response and autophagy through the circLrp1b/miR-27a-3p/Dram2 pathway in a rat model of traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hengchang Li,Chengxiang Lu,Wenfei Yao,Lixin Xu,Jun Zhou,Bin Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

Immunocytochemistry/ Immunofluorescence - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

Western blot - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

Western blot - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

Key facts

Reacts with

Target

Applications

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

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