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CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (ab252197) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (AB252197), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (AB252197), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (AB252197), expandable thumbnail

Key facts

Reactive species

Human

Associated Products

Select an associated product type

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CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (ab252197) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Key facts

Reactive species

Human

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

CD8 alpha Antibody Panel ab252197 contains multiple trial-sized versions of anti-human antibody clones against CD8 alpha, specifically selected for high performance in IHC. This panel contains 5 recombinant rabbit monoclonal antibodies against human CD8 alpha. They are provided in trial sizes to allow you to easily evaluate which is the best CD8 alpha antibody for your IHC research assay needs.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [CAL66] to CD8 alpha (20 μL) ab237709

- Rabbit monoclonal [CAL67] to CD8 alpha (20 μL) ab237710

- Rabbit monoclonal [SP16] to CD8 alpha (20 μL) ab101500

- Rabbit monoclonal [EPR22483-288] to CD8 alpha (20 μL) ab245118

- Rabbit monoclonal [EPR10640(2)] to CD8 alpha - N-terminal (20 μL) ab189926


Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.


Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

Associated diseases and disorders

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (ab252197), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (ab252197)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with Anti-CD8 alpha antibody [EPR22483-288] ab245118 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the T cell zone of human tonsil (PMID: 16504163) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (ab252197), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (ab252197)

    Immunohistochemical analysis of paraffin-embedded, Human tonsil tissue tissue labeling CD8 alpha with Anti-CD8 alpha antibody [EPR10640(2)] - N-terminal ab189926 at a 1/500 dilution. Counter stained with hematoxylin. Negative control also shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (ab252197), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD8 alpha Antibody Panel (SP16, CAL67, CAL66, EPR22483-288, EPR10640(2)) (ab252197)

    Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD8 with Anti-CD8 alpha antibody [SP16] ab101500 at 1/100 (21 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-CD8 alpha antibody [SP16] ab101500 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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