AB254017

Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human

Recombinant
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(2 Publications)

Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (ab254017) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

View Alternative Names

CD103, Integrin alpha-E, HML-1 antigen, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen, ITGAE

4 Images

Immunocytochemistry/ Immunofluorescence - Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (AB254017)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (AB254017)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling DC-SIGN + DC-SIGNR with ab245115 at 1/100 dilution, followed by a AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) seconday antibody (green) at 1/1000 dilution.

Confocal image showing cytoplasmic and membranous staining in THP-1 cells treated with 10 ng/ml PMA for 18 hours, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN expression is induced by PMA plus IL4 in THP-1(PMID : 15070901; PMID : 22675249). DC-SIGNR/CD299 expression is induced by PMA in THP-1 (PMID 30077333). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889)(red) at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (AB254017)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (AB254017)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling DC-SIGN + DC-SIGNR with ab245115 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining on dendritic cell-like cells of human skin (PMID : 11859097; PMID : 11859097) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (AB254017)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling CD103 with purified ab129202 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051 an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody (inset).

Counterstained with hematoxylin.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (AB254017)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100.

Key facts

Reacts with

Human

Target

ITGAE

target

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Product details

Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human ab254017 contains multiple trial-sized versions of anti-human antibody clones against CD103, LY75 / DEC-205, TLR7, DC-SIGN + DC-SIGNR, specifically selected for high performance in various applications. This panel contains 4 recombinant rabbit monoclonal antibodies against human CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [EPR4166(2)] to CD103 (20 μL) ab129202

- Rabbit monoclonal [EPR5233] to LY75/DEC-205 (20 μL) ab124897

- Rabbit monoclonal [EPR2088(2)] to TLR7 (20 μL) ab124928

- Rabbit monoclonal [EPR22395-72] to DC-SIGN + DC-SIGNR (20 μL) ab245115

Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab254017-1Kit", "assetComponentDetails": [ { "size":"2 x 10 µL", "name":"Anti-DC-SIGN + DC-SIGNR antibody [EPR22395-72]", "number":"AB254017-CMP03", "productcode":"AB245115" }, { "size":"2 x 10 µL", "name":"Anti-TLR7 antibody [EPR2088(2)]", "number":"AB254017-CMP01", "productcode":"AB124928" }, { "size":"2 x 10 µL", "name":"Anti-LY75 / DEC-205 antibody [EPR5233]", "number":"AB254017-CMP04", "productcode":"AB124897" }, { "size":"2 x 10 µL", "name":"Anti-CD103 antibody [EPR4166(2)]", "number":"AB254017-CMP02", "productcode":"AB129202" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LY75 also known as DEC-205 is a receptor that plays a role in antigen capture and presentation. It has a mass of about 205 kDa. Expressed on the surface of dendritic cells (DC) DEC-205 is considered an important marker for these immune cells. CD103 DC also use the DEC-205 pathway for their functions. Additionally DC-SIGN and its related receptor DC-SIGNR are other DC markers aiding in pathogen recognition. TLR7 part of the Toll-like receptor family is involved in the detection of single-stranded RNA contributing to immune responses.

Biological function summary

LY75/DEC-205 and associated DC markers function in the identification and processing of antigens. They recognize specific ligands and internalize them for processing and presentation to T cells. DEC-205 plays an important role in cross-presentation important for activating cytotoxic T cells. This receptor is part of a larger complex involving other C-type lectin receptors like DC-SIGN that are also present in dendritic cells. These receptors work together to enhance immune responses against pathogens.

Pathways

LY75/DEC-205 and related DC markers are integral to the antigen presentation pathways that activate T lymphocytes. These pathways include the major histocompatibility complex (MHC) class I and II pathways. DEC-205's role in the cross-presentation pathway allows DCs to present antigens to CD8+ T cells often in collaboration with CD103-mediated processes. Related proteins like CD40 and CD80/CD86 are also engaged during T cell priming enhancing immune activation.

LY75/DEC-205 has implications in autoimmunity and infectious diseases. Autoimmune conditions can arise when improper antigen presentation leads to self-reactive T cells. For example faulty presentation in DEC-205 pathways may link to diseases like rheumatoid arthritis. In infectious diseases receptors like DC-SIGN interact with pathogens such as HIV mediating viral entry and immune evasion. Proteins like TLR7 also connect to immune-related disorders by regulating responses to viral infections.

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Product protocols

Visit the General protocols
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Target data

The protein expressed by the ITGAE gene, known as integrin alpha-E/beta-7, functions as a receptor for E-cadherin and mediates the adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. This supplementary information is collated from multiple sources and compiled automatically.

See full target information ITGAE

Additional targets

LY75,TLR7,CD209,CLEC4M

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Immunity & ageing : I & A 21:56 PubMed39169358

2024

Mouse brain contains age-dependent extraparenchymal granular structures and astrocytes, both reactive to natural IgM antibodies, linked to the fissura magna.

Applications

Unspecified application

Species

Unspecified reactive species

Clara Romera,Marta Riba,Raquel Alsina,Marina Sartorio,Jordi Vilaplana,Carme Pelegrí,Jaume Del Valle

Journal for immunotherapy of cancer 10: PubMed34992092

2022

Intratumor expanded T cell clones can be non-sentinel lymph node derived in breast cancer revealed by single-cell immune profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Shiping Jiao,Qing Xiong,Meisi Yan,Xiaolu Zhan,Zhenhuang Yang,Cheng Peng,Beicheng Sun,Da Pang,Tong Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human

Immunocytochemistry/ Immunofluorescence - Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (AB254017)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (AB254017)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (AB254017)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dendritic Cell Marker (CD103, LY75/DEC-205, TLR7, DC-SIGN + DC-SIGNR) Antibody Panel - Human (AB254017)

Key facts

Reacts with

Target

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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