DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056) (ab103970) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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- Frontiers in oncology 11:6753972021High Expression of Cancer-IgG Is Associated With Poor Prognosis and Radioresistance PI3K/AKT/DNA-PKcs Pathway Regulation in Lung Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiongtao Yang,Guohui Wang,Jing You,Runchuan Gu,Xiaohong Xu,Changdan Xu,Hao Wang,Ruisong Zhao,Xiaoyan Qiu,Guangying ZhuPubMed 34150640
- Journal of dairy science 101:5317-53282018Nuclear factor erythroid 2-related factor 2-antioxidant activation through the action of ataxia telangiectasia-mutated serine/threonine kinase is essential to counteract oxidative stress in bovine mammary epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesY F Ma et. al.PubMed 29605311
- Cell cycle (Georgetown, Tex.) 9:1421-332010Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAneliya Velkova et. al.PubMed 20305393
- DNA repair 9:76-822009Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyo Sakasai,Hirobumi Teraoka,Randal S TibbettsPubMed 19959400
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DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056) (ab103970) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Notes
ab103970 is a DNA Damage Kinases Panel designed for the validation and characterization of the phosphorylation state of the DNA damage responsive kinases ATM and DNA-PKcs. ATM and DNA-PK are protein kinases that are active and become phosphorylated in response to DNA double strand breaks (DSBs). ATM is phosphorylated on S1981 and phosphorylates various proteins during the cellular response to DNA DSBs, while DNA-PK, a key component of the non-homologous end-joining pathway of DSB repair, is phosphorylated on S2056.
February 20th 2019: Please note these changes: The following changes were made to this panel: Anti-ATM antibody (ab82512) was replaced with Anti-ATM antibody [EPR17059] (ab199726), Anti-DNA PKcs (phospho S2056) antibody - ChIP Grade (ab18192) was replaced with Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (ab124918) and Anti-DNA PKcs antibody (ab70250) was replaced with Anti-DNA PKcs antibody [Y393] (ab32566).
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Western blot - DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056) (ab103970)
ab82512 was raised against an immunogen that is predicted to cross react with both isoform 1 (350 kDa) and isoform 2 (195 kDa) of Human Serine-protein kinase ATM (ATM). We believe that the band observed at 195 kDa corresponds to isoform 2.
Western blot - DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056) (ab103970)
All lanes: Western blot - Anti-ATM (phospho S1981) antibody [10H11.E12] (Anti-ATM (phospho S1981) antibody [10H11.E12] ab36810) at 10 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Extract from patient with Ataxia-Telangiectasia whole cell lysate at 20 µg
Lane 3: Irradiated HeLa Whole Cell Lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 351 kDa
Observed band size: 100 kDa, 110 kDa, 145 kDa, 200 kDa, 370 kDa
Exposure time: 20min
Western blot - DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056) (ab103970)
All lanes: Western blot - Anti-DNA PKcs antibody (Anti-DNA PKcs antibody ab70250) at 0.1 µg/mL
Lane 1: Whole cell lysate from Hela cells at 15 µg
Lane 2: Whole cell lysate from Hela cells at 50 µg
Predicted band size: 469 kDa
Observed band size: 170 kDa, 180 kDa, 230 kDa, 469 kDa
Western blot - DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056) (ab103970)
Anti-DNA PKcs (phospho S2056) antibody ab18192 specifically recognizes a band at ~460 kDa corresponding to DNA PKcs in HeLa cells that have been treated with ionizing radiation (lane 1). This band is not detected in untreated cells (lane 2). The activity of the antibody is quenched by the addition of the immunizing (modified) peptide, ab20406 (lanes 3) but not the unmodified peptide, Human DNA PKcs peptide ab20407 (lane 5).
For the ab13823 irradiated HeLa cell lysate, the 4 hour post-treatment extract was used.All lanes: Western blot - Anti-DNA PKcs (phospho S2056) antibody (Anti-DNA PKcs (phospho S2056) antibody ab18192) at 1 µg/mL
Lanes 1 and 5: Gamma Irradiated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lanes 2 and 6: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: Gamma Irradiated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with Human DNA PKcs (phospho S2056) peptide (ab20406)
Lane 4: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with Human DNA PKcs (phospho S2056) peptide (ab20406)
SecondaryAll lanes: Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 469 kDa
Observed band size: 270 kDa, 460 kDa
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