DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056)

• WB

Unknown

Western blot - DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056) (AB103970)

ab18192 specifically recognizes a band at ~460 kDa corresponding to DNA PKcs in HeLa cells that have been treated with ionizing radiation (lane 1). This band is not detected in untreated cells (lane 2). The activity of the antibody is quenched by the addition of the immunizing (modified) peptide, ab20406 (lanes 3) but not the unmodified peptide, ab20407 (lane 5). For the ab13823 irradiated HeLa cell lysate, the 4 hour post-treatment extract was used.

All lanes:

Western blot - Anti-DNA PKcs (phospho S2056) antibody (<a href='/en-us/products/primary-antibodies/dna-pkcs-phospho-s2056-antibody-ab18192'>ab18192</a>) at 1 µg/mL

Lanes 1 and 5:

Gamma Irradiated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 2 and 6:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Gamma Irradiated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with Human DNA PKcs (phospho S2056) peptide (<a href='/en-us/products/unavailable/human-dna-pkcs-phospho-s2056-peptide-ab20406'>ab20406</a>)

Lane 4:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with Human DNA PKcs (phospho S2056) peptide (<a href='/en-us/products/unavailable/human-dna-pkcs-phospho-s2056-peptide-ab20406'>ab20406</a>)

Secondary

All lanes:

Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

Predicted band size: 469 kDa

Observed band size: 270 kDa,460 kDa

true

• WB

Unknown

Western blot - DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056) (AB103970)

ab82512 was raised against an immunogen that is predicted to cross react with both isoform 1 (350 kDa) and isoform 2 (195 kDa) of Human Serine-protein kinase ATM (ATM). We believe that the band observed at 195 kDa corresponds to isoform 2.

false

• WB

Unknown

Western blot - DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056) (AB103970)

All lanes:

Western blot - Anti-ATM (phospho S1981) antibody [10H11.E12] (<a href='/en-us/products/primary-antibodies/atm-phospho-s1981-antibody-10h11e12-ab36810'>ab36810</a>) at 10 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Extract from patient with Ataxia-Telangiectasia whole cell lysate at 20 µg

Lane 3:

Irradiated HeLa Whole Cell Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 351 kDa

Observed band size: 100 kDa,110 kDa,145 kDa,200 kDa,370 kDa

true

Exposure time: 20min

• WB

Unknown

Western blot - DNA Damage Kinases Panel (ATM, ATM pS1981, ATR, ATR pS428, DNA-PKcs, DNA-PKcs pS2056) (AB103970)

All lanes:

Western blot - Anti-DNA PKcs antibody (<a href='/en-us/products/primary-antibodies/dna-pkcs-antibody-ab70250'>ab70250</a>) at 0.1 µg/mL

Lane 1:

Whole cell lysate from Hela cells at 15 µg

Lane 2:

Whole cell lysate from Hela cells at 50 µg

Predicted band size: 469 kDa

Observed band size: 170 kDa,180 kDa,230 kDa,469 kDa

false