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AB120474

Dynamin Inhibitors: Dynole™ Series Kit

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(7 Publications)

Dynamin Inhibitors: Dynole™ Series Kit (ab120474) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
1 Images
Immunocytochemistry/ Immunofluorescence - Dynamin Inhibitors: Dynole™ Series Kit (AB120474)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Dynamin Inhibitors: Dynole™ Series Kit (AB120474)

ab66705 staining PAI1 in HeLa cells treated with dynole-34-2™; (ab120463), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynole-34-2™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120463 (dynole-34-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Key facts

Applications

FuncS

applications

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

DynoleTM chemical series collection

Convenient kit containing dynamin inhibitor Dynole-34-2™ (ab120463; 1 mg) and negative control Dynole-31-2™ (ab120464; 1mg) from the Dynole chemical series. Dynole-34-2™ targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.

Target

Description

GTPase Allosteric Site

Dynole-34-2™:

Potent dynamin I and dynamin II inhibitor

GTPase Allosteric Site

Dynole-31-2™:

Negative control for Dynole 34-2™

Convenient kit containing dynamin inhibitor Dynole-34-2™ ab120464; 1mg) from the Dynole chemical series. Dynole-34-2™ targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.

Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole-34-2™, Dynole-31-2™ are trademarks of Children's Medical Research Institute and Newcastle Innovation Ltd.

Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole® is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab120474-1Kit", "assetComponentDetails": [ { "size":"1 x 1 mg", "name":"Dynole-31-2™", "number":"AB120474-CMP02", "productcode":"AB120464" }, { "size":"1 x 1 mg", "name":"Dynole-34-2™", "number":"AB120474-CMP01", "productcode":"AB120463" } ] } } }

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

Target data

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35852853

2022

Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation.

Applications

Unspecified application

Species

Unspecified reactive species

Cansu Küey,Méghane Sittewelle,Gabrielle Larocque,Miguel Hernández-González,Stephen J Royle

Viruses 12: PubMed33096814

2020

Oncolytic H-1 Parvovirus Enters Cancer Cells through Clathrin-Mediated Endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tiago Ferreira,Amit Kulkarni,Clemens Bretscher,Karsten Richter,Marcelo Ehrlich,Antonio Marchini

Journal of virology 93: PubMed30700597

2019

JC Polyomavirus Entry by Clathrin-Mediated Endocytosis Is Driven by β-Arrestin.

Applications

Unspecified application

Species

Unspecified reactive species

Colleen L Mayberry,Ashley N Soucy,Conner R Lajoie,Jeanne K DuShane,Melissa S Maginnis

PloS one 12:e0182589 PubMed28787011

2017

An antipsychotic drug exerts anti-prion effects by altering the localization of the cellular prion protein.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Stincardini,Tania Massignan,Silvia Biggi,Saioa R Elezgarai,Valeria Sangiovanni,Ilaria Vanni,Michael Pancher,Valentina Adami,Jorge Moreno,Matteo Stravalaci,Giulia Maietta,Marco Gobbi,Alessandro Negro,Jesús R Requena,Joaquín Castilla,Romolo Nonno,Emiliano Biasini

Traffic (Copenhagen, Denmark) 17:21-39 PubMed26459807

2015

HSV-1 Glycoproteins Are Delivered to Virus Assembly Sites Through Dynamin-Dependent Endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Albecka,Romain F Laine,Anne F J Janssen,Clemens F Kaminski,Colin M Crump

The Journal of cell biology 203:315-26 PubMed24145164

2013

Lipid droplet breakdown requires dynamin 2 for vesiculation of autolysosomal tubules in hepatocytes.

Applications

FuncS

Species

Unspecified reactive species

Ryan J Schulze,Shaun G Weller,Barbara Schroeder,Eugene W Krueger,Susan Chi,Carol A Casey,Mark A McNiven

Journal of medicinal chemistry 52:3762-73 PubMed19459681

2009

Inhibition of dynamin mediated endocytosis by the dynoles--synthesis and functional activity of a family of indoles.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy A Hill,Christopher P Gordon,Andrew B McGeachie,Barbara Venn-Brown,Luke R Odell,Ngoc Chau,Annie Quan,Anna Mariana,Jennette A Sakoff,Megan Chircop,Phillip J Robinson,Adam McCluskey
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