Dynamin Inhibitors: Dynole™ Series Kit (ab120474) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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- eLife 11:2022Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCansu Küey,Méghane Sittewelle,Gabrielle Larocque,Miguel Hernández-González,Stephen J RoylePubMed 35852853
- Viruses 12:2020Oncolytic H-1 Parvovirus Enters Cancer Cells through Clathrin-Mediated Endocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTiago Ferreira et. al.PubMed 33096814
- Journal of virology 93:2019JC Polyomavirus Entry by Clathrin-Mediated Endocytosis Is Driven by β-Arrestin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColleen L Mayberry et. al.PubMed 30700597
- PloS one 12:e01825892017An antipsychotic drug exerts anti-prion effects by altering the localization of the cellular prion protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia Stincardini et. al.PubMed 28787011
- Traffic (Copenhagen, Denmark) 17:21-392015HSV-1 Glycoproteins Are Delivered to Virus Assembly Sites Through Dynamin-Dependent Endocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Albecka,Romain F Laine,Anne F J Janssen,Clemens F Kaminski,Colin M CrumpPubMed 26459807
- The Journal of cell biology 203:315-262013Lipid droplet breakdown requires dynamin 2 for vesiculation of autolysosomal tubules in hepatocytes.Applications:FuncSReactive species:Unspecified reactive speciesRyan J Schulze et. al.PubMed 24145164
- Journal of medicinal chemistry 52:3762-732009Inhibition of dynamin mediated endocytosis by the dynoles--synthesis and functional activity of a family of indoles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy A Hill,Christopher P Gordon,Andrew B McGeachie,Barbara Venn-Brown,Luke R Odell,Ngoc Chau,Annie Quan,Anna Mariana,Jennette A Sakoff,Megan Chircop,Phillip J Robinson,Adam McCluskeyPubMed 19459681
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Dynamin Inhibitors: Dynole™ Series Kit (ab120474) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Applications
- FuncS
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
- DynoleTM chemical series collection
Convenient kit containing dynamin inhibitor Dynole-34-2™ (ab120463; 1 mg) and negative control Dynole-31-2™ (ab120464; 1mg) from the Dynole chemical series. Dynole-34-2™ targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.
Target
Description
GTPase Allosteric Site
Dynole-34-2™:
Potent dynamin I and dynamin II inhibitor
GTPase Allosteric Site
Dynole-31-2™:
Negative control for Dynole 34-2™
Convenient kit containing dynamin inhibitor Dynole-34-2™ ab120464; 1mg) from the Dynole chemical series. Dynole-34-2™ targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole-34-2™, Dynole-31-2™ are trademarks of Children's Medical Research Institute and Newcastle Innovation Ltd.
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole® is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.
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1 product image
Immunocytochemistry/ Immunofluorescence - Dynamin Inhibitors: Dynole™ Series Kit (ab120474)
Anti-PAI1 antibody ab66705 staining PAI1 in HeLa cells treated with dynole-34-2™; (Dynole® 34-2, dynamin I and dynamin II inhibitor ab120463), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynole-34-2™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of Dynole® 34-2, dynamin I and dynamin II inhibitor ab120463 (dynole-34-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-PAI1 antibody ab66705 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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