- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Dynamin Inhibitors Toolbox (AB120468)
ab66705 staining PAI1 in HeLa cells treated with OcTMAB™ (ab120467), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of OcTMAB™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120467 (OcTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Dynamin Inhibitors Toolbox (AB120468)
ab66705 staining PAI1 in HeLa cells treated with MiTMAB™ (ab120466), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of MiTMAB™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120466 (MiTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Dynamin Inhibitors Toolbox (AB120468)
ab66705 staining PAI1 in HeLa cells treated with dynole-34-2™; (ab120463), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynole-34-2™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120463 (dynole-34-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Complementary Products
Reactivity data
Product details
The Dynamin Inhibitors Toolbox (ab120468) is a unique collection of small molecule, potent, and cell permeable dynamin inhibitors, enabling characterization of dynamin and endocytosis. The inhibitors have different mechanisms of action, target different domains of dynamin, and are based on differing chemical scaffolds.
Negative controls for each chemical series are included.
The Toolbox contains 1mg of each of the following: MiTMAB™ (ab120466), OcTMAB™ (ab120467), Pro-Mystyric Acid (ab120476), Dynole-34-2™ (ab120463) and Dynole-31-2™ (ab120464).
MiTMABTM chemical series collection
Contains MiTMABTM (ab120466), OcTMABTM (ab120467) and Pro-Myristic Acid (ab120476). OcTMABTM and MiTMABTM target dynamin at the lipid binding (PH) domain and inhibit dynamin and endocytosis in enzymatic and cell based assays. They are based on the same chemical scaffold.
Pro-Myristic acid is an in vitro inhibitor of dynamin. Although cell permeable, it is rapidly broken down by cellular esterases to release intracellular myristic acid, which is not a dynamin inhibitor. It can therefore be used as a negative control in cell-based studies.
DynoleTM chemical series collection
Contains dynamin inhibitor Dynole-34-2TM (ab120463) and negative control Dynole-31TM (ab120464) from the Dynole chemical series. Dynole-34-2TM targets dynamin at the GTPase Allosteric Site (GAS) domain and inhibits dynamin and endocytosis in enzymatic and cell based assays. The inhibitor and negative control are based on the same chemical scaffold.
PH lipid binding site
MiTMAB™ - Cell-permeable dynamin I and dynamin II inhibitor
PH lipid binding site
OcTMAB™ - Cell permeable dynamin I and dynamin II inhibitor
PH lipid binding site
Pro-Myristic Acid - Negative control for MiTMAB™ and OcTMAB™
GTPase Allosteric Site
Dynole-34-2™ - Potent dynamin I and dynamin II inhibitor
GTPase Allosteric Site
Dynole-31-2™ - Negative control for Dynole 34-2™
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole-34-2™, Dynole-31-2™, MiTMAB™ and OcTMAB™ are trademarks of Children's Medical Research Institute and Newcastle Innovation Ltd.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Target data
Publications (6)
Recent publications for all applications. Explore the full list and refine your search
Developmental cell 45:738-752.e6 PubMed29920278
2018
Applications
Unspecified application
Species
Unspecified reactive species
American journal of physiology. Regulatory, integrative and comparative physiology 310:R570-7 PubMed26791826
2016
Applications
Unspecified application
Species
Unspecified reactive species
Methods in molecular biology (Clifton, N.J.) 1174:361-73 PubMed24947395
2014
Applications
Unspecified application
Species
Unspecified reactive species
Molecular cancer therapeutics 9:1995-2006 PubMed20571068
2010
Applications
Unspecified application
Species
Unspecified reactive species
Journal of medicinal chemistry 53:4094-102 PubMed20426422
2010
Applications
Unspecified application
Species
Unspecified reactive species
Journal of medicinal chemistry 52:3762-73 PubMed19459681
2009
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com