Embryonic Stem Cell Marker Panel containing 5 antibodies against Human Oct4, Nanog, Tra-1-60 (R), SOX2 and SSEA4
- Designed for the validation and characterization of cultured or newly derived human embryonic stem (ES) cell lines
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- Translational neurodegeneration 12:312023Reciprocal effects of alpha-synuclein aggregation and lysosomal homeostasis in synucleinopathy models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlice Drobny,Fanni Annamária Boros,Denise Balta,Susy Prieto Huarcaya,Deniz Caylioglu,Niyeti Qazi,Julia Vandrey,Yanni Schneider,Jan Philipp Dobert,Caleb Pitcairn,Joseph Robert Mazzulli,Friederike ZunkePubMed 37312133
- Stem cell reviews and reports 17:2223-22342021Identification of New Transcription Factors that Can Promote Pluripotent Reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Huang,Jieying Zhu,Yu Liu,Guihuan Liu,Ran Zhang,Dongwei Li,Duanqing Pei,Ping ZhuPubMed 34448118
- Theranostics 10:11215-112292020Intracellular calcium current disorder and disease phenotype in mutant iPSC-based cardiomyocytes in arrhythmogenic right ventricular cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeipei Chen et. al.PubMed 33042279
- Journal of translational medicine 18:2362020Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Jin Park et. al.PubMed 32532292
- American journal of physiology. Heart and circulat 318:H801-H8152020DNA damage-free iPS cells exhibit potential to yield competent cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica M Miller et. al.PubMed 32057252
- Bio-protocol 9:e31882019Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesScott Bell,Nuwan C Hettige,Heika Silveira,Huashan Peng,Hanrong Wu,Malvin Jefri,Lilit Antonyan,Ying Zhang,Xin Zhang,Carl ErnstPubMed 33654990
- eLife 6:2017Patient-specific mutations impair BESTROPHIN1's essential role in mediating Ca-dependent Cl currents in human RPE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Li et. al.PubMed 29063836
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Embryonic Stem Cell Marker Panel containing 5 antibodies against Human Oct4, Nanog, Tra-1-60 (R), SOX2 and SSEA4
- Designed for the validation and characterization of cultured or newly derived human embryonic stem (ES) cell lines
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- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
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Product Specifications
Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (ab109884) is a panel of 5 antibodies designed for the validation and characterization of cultured or newly derived human embryonic stem (ES) cell lines. Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique: Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).
This panel contains 3 monoclonal antibodies and 2 polyclonal antibodies to Oct4, SOX2, SSEA4, Nanog and TRA-1-60 (R) in convient trial sizes (50 µg/ 50 µL) to allow for efficient and cost-effect testing of the antibodies simultaneously.
The individual antibodies included in this panel are:- Rabbit polyclonal anti-Oct4 antibody (ab19857)
- Rabbit polyclonal anti-SOX2 antibody (ab97959)
- Mouse monoclonal anti-SSEA4 antibody [MC813-70] (ab16287)
- Rabbit recombinant monoclonal anti-Nanog antibody [EPR2027(2)] (ab109250)
- Mouse monoclonal anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
All of these component antibodies are available for purchase individually using their corresponding abIDs.
Quality and Validation
Abcams high quality validation processes ensure each component antibody is sensitive and specific to the target protein.
All antibodies in this panel have been validated in human samples in a variety of applications, and some also have reactivity in other species.
For information on the species reactivity and application validation of each antibody within the panel, please consult the individual datasheet for each antibody. Suggested dilution factor and additional information on protocols can also be found on the antibody datasheets.
All antibodies in this panel have a minimum of 130 citations in peer-reviewed journals and are trusted by scientific community.
Related Products
Many of our antibody clones are also available as carrier-free formulations for easy conjugation to labels of your choice, or in pre-conjugated formats. Please search the clone name for alternative formulations and related products.Panel contains: designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
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We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (ab109884)
Immunocytochemistry/Immunofluorescence analysis of NCCIT(human pluripotent embryonal carcinoma) cells labelling Nanog with purified Anti-Nanog antibody [EPR2027(2)] ab109250 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
This image is courtesy of an anonymous collaborator.Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (ab109884)
This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody Anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody Anti-TRA-1-60 (R) antibody [TRA-1-60] ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.
This image is courtesy of an anonymous collaborator.Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (ab109884)
ICC/IF image of Sox2 (Anti-SOX2 antibody ab97959) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.01% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-SOX2 antibody ab97959, 1/500 dilution) overnight at +4°C.
This image is courtesy of an anonymous collaborator.Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (ab109884)
NTERA2 Human Embryonic Carcinoma cells were stained with SSEA4 antibody Anti-SSEA4 antibody [MC813-70] ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.
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