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AB107156

Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1)

5

(1 Review)

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(9 Publications)

Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (ab107156) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
3 Images
Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (AB107156)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (AB107156)

ICC/IF image of Sox2 (ab97959, green) and SSEA1 (ab16285, red) co-stained mouse embryonic stem cells. The cells were fixed in paraformaldehyde and then permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The cells were then incubated with Sox2 (1 : 500) and SSEA1 (1 : 10) overnight at 4°C. The secondary antibodies, alexa fluor 488 goat anti rabbit IgG (green) and alexa fluor 647 goat anti mouse IgM (red), were both use at a 1 : 500 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (AB107156)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (AB107156)

ICC/IF image of Oct4 (ab19857, green) and SSEA1 (ab16285, red) co-stained mouse embryonic stem cells. The cells were fixed in 4% paraformaldehyde and then permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The cells were then incubated with Oct4 (1 : 250) and SSEA1 (1 : 10) overnight at 4°C. The secondary antibodies, alexa fluor 488 goat anti rabbit IgG (green) and alexa fluor 647 goat anti mouse IgM (red), were both use at a 1 : 500 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (AB107156)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (AB107156)

ab16285 staining SSEA1 (red) in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The primary antibody was diluted 1 : 10 and incubated with the sample overnight at 4°C. An Alexa Fluor 647 conjugated goat anti mouse IgM antibody, diluted 1 : 500, was used as the secondary and incubated with samples for 1 hour at room temperature.

Key facts

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Complementary Products

Primary Antibodies

AB214549

Anti-Nanog antibody [EPR20694] - ChIP Grade

RabMAb|Advanced Validation|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Nanog antibody [EPR20694] - ChIP Grade (AB214549)

  • 5
  • 3
Primary Antibodies

AB19857

Anti-Oct4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (AB19857)

  • 4
  • 45
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

  • 4
  • 75
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Product details

ab107156 is a Mouse Embryonic Stem Cell Marker Panel containing four antibodies: 50μg of Oct4 rabbit polyclonal, 50μl of Nanog rabbit polyclonal, 50μg of SOX2 rabbit polyclonal and 50μl of SSEA1 mouse monoclonal.

The Mouse Embryonic Stem Cell Marker Panel is designed for the validation and characterization of cultured or newly derived mouse embryonic stem (ES) cell lines. The panel contains four antibodies to well known markers of mouse ES cells (Oct4, Nanog, SOX2 and SSEA1). Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique. Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).

The antibodies in this panel were selected for their exceptional performance in ICC/IF in mouse cells. In addition, these antibodies have also been tested in a number other applications and species. Please see the individual datasheets for additional information. Recommended working dilutions for ICC/IF in mouse can also be found in the figure legends on this datasheet.

designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

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What's included?

{ "values": { "1Unit": { "sellingSize": "1 Unit", "publicAssetCode":"ab107156-1Unit", "assetComponentDetails": [ { "size":"1 x 50 µg", "name":"Anti-SOX2 antibody", "number":"AB107156-CMP02" }, { "size":"1 x 50 µg", "name":"Anti-Oct4 antibody - ChIP Grade", "number":"AB107156-CMP04" }, { "size":"1 x 50 µL", "name":"Anti-SSEA1 antibody [MC-480]", "number":"AB107156-CMP03" }, { "size":"1 x 50 µL", "name":"Anti-Nanog antibody [EPR20694] - ChIP Grade", "number":"AB107156-CMP05" } ] }, "1Unit": { "sellingSize": "1 Unit", "publicAssetCode":"ab107156-1Unit", "assetComponentDetails": [ { "size":"1 x 50 µg", "name":"Anti-SOX2 antibody", "number":"AB107156-CMP02", "productcode":"AB97959" }, { "size":"1 x 50 µg", "name":"Anti-Oct4 antibody - ChIP Grade", "number":"AB107156-CMP04", "productcode":"AB19857" }, { "size":"1 x 50 µL", "name":"Anti-Nanog antibody", "number":"AB107156-CMP01", "productcode":"AB80892" }, { "size":"1 x 50 µL", "name":"Anti-SSEA1 antibody [MC-480]", "number":"AB107156-CMP03", "productcode":"AB16285" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
-20°C
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Product protocols

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Target data

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4682 PubMed37542049

2023

The AT-hook is an evolutionarily conserved auto-regulatory domain of SWI/SNF required for cell lineage priming.

Applications

Unspecified application

Species

Unspecified reactive species

Dhurjhoti Saha,Solomon Hailu,Arjan Hada,Junwoo Lee,Jie Luo,Jeff A Ranish,Yuan-Chi Lin,Kyle Feola,Jim Persinger,Abhinav Jain,Bin Liu,Yue Lu,Payel Sen,Blaine Bartholomew

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 39:1622-1632 PubMed33140859

2020

Chondrocyte-to-osteoblast transformation in mandibular fracture repair.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah A Wong,Diane P Hu,Joshua Slocum,Charles Lam,Michael Nguyen,Theodore Miclau,Ralph S Marcucio,Chelsea S Bahney

The Journal of biological chemistry 294:7769-7786 PubMed30926604

2019

The extracellular domain of epithelial cell adhesion molecule (EpCAM) enhances multipotency of mesenchymal stem cells through EGFR-LIN28-LET7 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

I-I Kuan,Chi-Chiu Lee,Chien-Hsu Chen,Jean Lu,Yuan-Sung Kuo,Han-Chung Wu

EMBO reports 19: PubMed30389724

2018

KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Yang,Lishuang Song,Xuefei Liu,Lige Bai,Guangpeng Li

Scientific reports 7:41852 PubMed28157205

2017

EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2α.

Applications

Unspecified application

Species

Unspecified reactive species

I-I Kuan,Kang-Hao Liang,Yi-Ping Wang,Ting-Wen Kuo,Yaa-Jyuhn James Meir,Sareina Chiung-Yuan Wu,Shang-Chih Yang,Jean Lu,Han-Chung Wu

Stem cells and development 25:803-13 PubMed27021700

2016

Differentiation of Spiral Ganglion-Derived Neural Stem Cells into Functional Synaptogenetic Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyang Li,Alicia Aleardi,Jue Wang,Yang Zhou,Rodrigo Andrade,Zhengqing Hu

Nature 463:621-6 PubMed20054295

2010

Opposing microRNA families regulate self-renewal in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Collin Melton,Robert L Judson,Robert Blelloch

Genes & development 23:837-48 PubMed19339689

2009

A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Hu,Jonghwan Kim,Qikai Xu,Yumei Leng,Stuart H Orkin,Stephen J Elledge

Nature 453:223-7 PubMed18362916

2008

REST maintains self-renewal and pluripotency of embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjay K Singh,Mohamedi N Kagalwala,Jan Parker-Thornburg,Henry Adams,Sadhan Majumder
View all publications
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