Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (ab107156) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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- Nature communications 14:46822023The AT-hook is an evolutionarily conserved auto-regulatory domain of SWI/SNF required for cell lineage priming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDhurjhoti Saha,Solomon Hailu,Arjan Hada,Junwoo Lee,Jie Luo,Jeff A Ranish,Yuan-Chi Lin,Kyle Feola,Jim Persinger,Abhinav Jain,Bin Liu,Yue Lu,Payel Sen,Blaine BartholomewPubMed 37542049
- The Journal of biological chemistry 294:7769-77862019The extracellular domain of epithelial cell adhesion molecule (EpCAM) enhances multipotency of mesenchymal stem cells through EGFR-LIN28-LET7 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesI-I Kuan et. al.PubMed 30926604
- EMBO reports 19:2018KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Yang et. al.PubMed 30389724
- Scientific reports 7:418522017EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesI-I Kuan et. al.PubMed 28157205
- Stem cells and development 25:803-132016Differentiation of Spiral Ganglion-Derived Neural Stem Cells into Functional Synaptogenetic Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyang Li et. al.PubMed 27021700
- Nature 463:621-62010Opposing microRNA families regulate self-renewal in mouse embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCollin Melton et. al.PubMed 20054295
- Genes & development 23:837-482009A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuang Hu,Jonghwan Kim,Qikai Xu,Yumei Leng,Stuart H Orkin,Stephen J ElledgePubMed 19339689
- Nature 453:223-72008REST maintains self-renewal and pluripotency of embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanjay K Singh et. al.PubMed 18362916
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Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (ab107156) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Notes
ab107156 is a Mouse Embryonic Stem Cell Marker Panel containing four antibodies: 50μg of Oct4 rabbit polyclonal, 50μl of Nanog rabbit polyclonal, 50μg of SOX2 rabbit polyclonal and 50μl of SSEA1 mouse monoclonal.
The Mouse Embryonic Stem Cell Marker Panel is designed for the validation and characterization of cultured or newly derived mouse embryonic stem (ES) cell lines. The panel contains four antibodies to well known markers of mouse ES cells (Oct4, Nanog, SOX2 and SSEA1). Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique. Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).
The antibodies in this panel were selected for their exceptional performance in ICC/IF in mouse cells. In addition, these antibodies have also been tested in a number other applications and species. Please see the individual datasheets for additional information. Recommended working dilutions for ICC/IF in mouse can also be found in the figure legends on this datasheet.
designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
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3 product images
Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (ab107156)
ICC/IF image of Oct4 (Anti-Oct4 antibody ab19857, green) and SSEA1 (Anti-SSEA1 antibody [MC-480] ab16285, red) co-stained mouse embryonic stem cells. The cells were fixed in 4% paraformaldehyde and then permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The cells were then incubated with Oct4 (1:250) and SSEA1 (1:10) overnight at 4°C. The secondary antibodies, alexa fluor 488 goat anti rabbit IgG (green) and alexa fluor 647 goat anti mouse IgM (red), were both use at a 1:500 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (ab107156)
ICC/IF image of Sox2 (Anti-SOX2 antibody ab97959, green) and SSEA1 (Anti-SSEA1 antibody [MC-480] ab16285, red) co-stained mouse embryonic stem cells. The cells were fixed in paraformaldehyde and then permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The cells were then incubated with Sox2 (1:500) and SSEA1 (1:10) overnight at 4°C. The secondary antibodies, alexa fluor 488 goat anti rabbit IgG (green) and alexa fluor 647 goat anti mouse IgM (red), were both use at a 1:500 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Mouse: Oct4, Nanog, SOX2, SSEA1) (ab107156)
Anti-SSEA1 antibody [MC-480] ab16285 staining SSEA1 (red) in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The primary antibody was diluted 1:10 and incubated with the sample overnight at 4°C. An Alexa Fluor 647 conjugated goat anti mouse IgM antibody, diluted 1:500, was used as the secondary and incubated with samples for 1 hour at room temperature.
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