Skip to main content

EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (AB216833), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (AB216833), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (AB216833), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (AB216833), expandable thumbnail

Publications

Key facts

Reactive species
Human

Associated Products

Select an associated product type

8 products for Alternative Product

Recommended products

EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Key facts

Reactive species
Human

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols

Notes

EMT Marker / Epithelial to Mesenchymal Marker antibody sampler panel ab216833 contains high performing antibodies against the key EMT markers: E-cadherin, N-cadherin, Snail + Slug, and Vimentin. It also contains an anti-rabbit secondary antibody.

EMT is a reversible process where epithelial cells undergo defined molecular changes to become motile mesenchymal cells. This EMT marker sampler panel is a cost-effective and convenient means of evaluating EMT.

The antibodies in this panel were selected for their exceptional performance in IHC in human tissues. These antibodies have also been tested in a number other applications and species. Please see the individual datasheets for additional information.

We do not recommend the use of SNAIL + SLUG antibody ab85936 for western blot (note: the other antibodies in this panel perform well in western blot). For SNAIL + SLUG in western blot, we recommend ab180714.

Panel contents:

E-cadherin rabbit monoclonal ab40772 - 10 μl
N-cadherin rabbit monoclonal ab76011 - 10 μl
Snail + Slug rabbit polyclonal ab85936 - 10 μg
Vimentin rabbit monoclonal ab92547 - 10 μl
anti-rabbit secondary antibody (HRP) ab205718 - 100 μg


Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833)

    Anti-N Cadherin antibody [EPR1791-4] ab76011 staining N Cadherin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA + 1% FBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833)

    IHC image of Anti-SNAIL + SLUG antibody ab85936 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-SNAIL + SLUG antibody ab85936, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833)

    Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker ab40772. Green-E-Cadherin red-PI

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833)

    IHC image of unpurified Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 staining Vimentin in human breast adenocarcinoma formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com