EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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- Aging 15:11860-118742023Lipid reprogramming induced by the NNMT-ABCA1 axis enhanced membrane fluidity to promote endometrial cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQirong Wen,Xiaohui Xie,Caiyuan Chen,Bolun Wen,Yaqiong Liu,Jie Zhou,Xiaobin Lin,Han Jin,Kun ShiPubMed 37889548
- Theranostics 11:3932-39472021Exosomal miR-500a-5p derived from cancer-associated fibroblasts promotes breast cancer cell proliferation and metastasis through targeting USP28.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Chen,Yuting Sang,Xiaojin Song,Dong Zhang,Lijuan Wang,Wenjing Zhao,Yiran Liang,Ning Zhang,Qifeng YangPubMed 33664871
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- Human
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EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Reactive species
- Human
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Please refer to protocols
Notes
EMT Marker / Epithelial to Mesenchymal Marker antibody sampler panel ab216833 contains high performing antibodies against the key EMT markers: E-cadherin, N-cadherin, Snail + Slug, and Vimentin. It also contains an anti-rabbit secondary antibody.
EMT is a reversible process where epithelial cells undergo defined molecular changes to become motile mesenchymal cells. This EMT marker sampler panel is a cost-effective and convenient means of evaluating EMT.
The antibodies in this panel were selected for their exceptional performance in IHC in human tissues. These antibodies have also been tested in a number other applications and species. Please see the individual datasheets for additional information.
We do not recommend the use of SNAIL + SLUG antibody ab85936 for western blot (note: the other antibodies in this panel perform well in western blot). For SNAIL + SLUG in western blot, we recommend ab180714.
Panel contents:
E-cadherin rabbit monoclonal ab40772 - 10 μl
N-cadherin rabbit monoclonal ab76011 - 10 μl
Snail + Slug rabbit polyclonal ab85936 - 10 μg
Vimentin rabbit monoclonal ab92547 - 10 μl
anti-rabbit secondary antibody (HRP) ab205718 - 100 μgExplore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
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4 product images
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833)
Anti-N Cadherin antibody [EPR1791-4] ab76011 staining N Cadherin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA + 1% FBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833)
IHC image of Anti-SNAIL + SLUG antibody ab85936 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-SNAIL + SLUG antibody ab85936, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833)
Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker ab40772. Green-E-Cadherin red-PI
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EMT Marker / Epithelial to Mesenchymal Transition Marker Panel (ab216833)
IHC image of unpurified Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 staining Vimentin in human breast adenocarcinoma formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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