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Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Key facts

Reactive species

Human

Target

CD31

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Target data

Function

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity).Isoform Delta15Does not protect against apoptosis.

Alternative names

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Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Alternative names

Key facts

Reactive species

Human

Target

CD31

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human ab254023 contains multiple trial-sized versions of anti-human antibody clones against CD31, CD34, ICAM1, CD45, specifically selected for high performance in various applications. This panel contains 4 recombinant rabbit monoclonal antibodies against human CD31, CD34, ICAM1, CD45 and 1 mouse monoclonal against human CD31. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [EP3095] to CD31 (20 μL) ab134168

- Mouse monoclonal [P2B1] to CD31 (20 μg) ab24590

- Rabbit monoclonal [EP373Y] to CD34 (20 μL) ab81289

- Rabbit monoclonal [EP1442Y] to ICAM1 (20 μL) ab53013

- Rabbit monoclonal [EP322Y] to CD45 (20 μL) ab40763


Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.


Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

Biological function summary

These proteins act in various roles essential for cellular communication and immune response regulation. ICAM1 functions as a ligand for integrins facilitating the adhesion and transmigration of leukocytes through the endothelium. CD34 plays a role in cell adhesion and differentiation marking hematopoietic precursor cells. CD45 is significant in signal transduction for leukocytes functioning in antigen receptor signaling pathways. CD31 mediates leukocyte transmigration and is engaged in maintaining vascular barrier integrity. These proteins are not typically part of a larger complex but interact with various receptors and ligands in their respective functions.

Activity summary

ICAM1 CD34 CD45 and CD31 are important proteins involved in cell communication and adhesion. ICAM1 also known as CD54 is a cell surface glycoprotein with a mass of about 55-60 kDa and is expressed on endothelial cells and immune cells. CD34 is a sialomucin-like protein expressed on hematopoietic stem cells and endothelial cells. CD45 also known as leukocyte common antigen (LCA) is a receptor-type protein tyrosine phosphatase with a mass of around 180-240 kDa and occurs universally on nucleated hematopoietic cells. CD31 known as platelet endothelial cell adhesion molecule (PECAM-1) is found on endothelial cells some immune cells and platelets. It has a mass of approximately 130 kDa. These proteins are endothelial markers useful in immunohistochemistry for identifying specific cell types.

Pathways

These proteins contribute to important biological processes including the immune response and hematopoiesis. ICAM1 is involved in the immune response pathway facilitating interactions between leukocytes and endothelial cells. This involves integrins such as LFA-1 which aid in leukocyte adhesion. CD45 is important in lymphocyte activation pathways interacting with antigen receptors for T and B cell activation. CD34 is involved in hematopoiesis via the stem cell niche interactions. CD31 plays a role in the vascular integrity pathway. Together these proteins integrate into broader cellular signaling networks for effective immune surveillance and response.

Associated diseases and disorders

These proteins contribute to the pathology of conditions like atherosclerosis and leukemia. ICAM1 expression increases in inflammatory disorders contributing to atherosclerosis by facilitating leukocyte adhesion to endothelium. This process often involves other proteins like VCAM-1 in promoting atherogenesis. CD34 is a marker for certain hematological malignancies including acute myeloid leukemia serving as a hematopoietic stem cell marker. CD45 is relevant in identifying leukocyte populations in leukemias. CD31 with its role in endothelial integrity can be implicated in vascular disorders. These proteins are useful in diagnostic contexts aiding in the identification and characterization of these disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023)

    Immunocytochemistry/Immunofluorescence analysis of HUVEC (Human umbilical vein endothelial cell line) cells labelling CD34 with purified ab81289 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain.

    Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human angiosarcoma labeling CD34 with unpurified ab81289 at 1/100-1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Endothelial Cell Marker (CD31, CD34, ICAM1, CD45) Antibody Sampler Panel - Human (ab254023)

    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma vessels tissue using ab134168 showing +ve staining.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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