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AB269810

EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC

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(1 Publication)

EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (ab269810) is part of our antibody panels range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)

IHC image of ab20160 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20160, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling EpCAM with ab213500 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membranous staining on human colon is observed [PMID : 15637741].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)

Immunohistochemical analysis of paraffin-embedded human endometrium cancer tissue labeling EpCAM with ab213500 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membranous staining on tumor cells of human endometrium cancer is observed [PMID : 15637741].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling EpCAM with ab223582 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human colon is observed (PMID : 15637741). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling EpCAM with ab223582 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human thyroid cancer is observed (PMID : 15637741). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC (AB269810)

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human endometrial adenocarcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Key facts

Reacts with

Human

Applications

IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

EpCAM (EPR20532-225, AUA1, EPR20532-222, EPR677(2)) Antibody Sampler Panel - Human, IHC ab269810 contains multiple trial-sized versions of anti-human antibody clones against EpCAM, specifically selected for high performance in IHC. This panel contains 3 recombinant rabbit and 1 mouse monoclonal antibodies against human EpCAM. They are provided as a sampler panel to allow you to easily evaluate each antibody.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

Recombinant Anti-EpCAM antibody [EPR20532-225] (20 μL) ab223582

Anti-EpCAM antibody [AUA1] (20 μg) ab20160

Recombinant Anti-EpCAM antibody [EPR20532-222] (20 μL) ab213500

Recombinant Anti-EpCAM antibody [EPR677(2)] (20 μL) ab124825

Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab269810-1Kit", "assetComponentDetails": [ { "size":"2 x 10 µg", "name":"Anti-EpCAM antibody [AUA1]", "number":"AB269810-CMP01", "productcode":"AB20160" }, { "size":"2 x 10 µL", "name":"Recombinant Anti-EpCAM antibody [EPR677(2)]", "number":"AB269810-CMP02", "productcode":"AB124825" }, { "size":"2 x 10 µL", "name":"Recombinant Anti-EpCAM antibody [EPR20532-225]", "number":"AB269810-CMP04", "productcode":"AB223582" }, { "size":"2 x 10 µL", "name":"Recombinant Anti-EpCAM antibody [EPR20532-222]", "number":"AB269810-CMP03", "productcode":"AB213500" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:13264 PubMed40246912

2025

Immune response against antibiotic-resistant and antibiotic-sensitive staphylococcus aureus in a rat model of implant infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfang Fan,Amita Sekar,Madeline McCanne,Jean Yuh,Devika Dutta Kannambadi,Sashank Lekkala,Orhun K Muratoglu,Ebru Oral
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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