Fibroblast Marker panel containing 4 antibodies against Vimentin, alpha smooth muscle Actin, Hsp47, S100A4
- Provided as a sampler panel to allow you to easily evaluate each in your required applications.
Images
Publications
Filter by
- Current issues in molecular biology 44:5815-58262022Antifibrosis Efficacy of Apo-9-Fucoxanthinone-Contained Ethanol Extract on Nasal Polyp: An In Vitro and Ex Vivo Organ Culture Assay.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMi-Jin Yim,Jeong Min Lee,Seok-Chun Ko,Hyun-Soo Kim,Ji-Yul Kim,Seong Kook Park,Dae-Sung Lee,Il-Whan ChoiPubMed 36421679
- Biochemistry research international 2020:88929302020In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaoyuan Xu,Jie Li,Xiaoyan Chen,Beiyu LiuPubMed 33149950
Key facts
- Reactive species
- Mouse, Human
- Target
- VIM
(See target data below)
Abcam Recommends
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.
Additional Targets
SERPINH1, VIM, ACTA2, S100A4, SERPINH1, S100A4, ACTA2, ACTG2
Alternative names
Vimentin, VIM
What's included?
Recommended products
Fibroblast Marker panel containing 4 antibodies against Vimentin, alpha smooth muscle Actin, Hsp47, S100A4
- Provided as a sampler panel to allow you to easily evaluate each in your required applications.
Key facts
- Reactive species
- Mouse, Human
- Target
- VIM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Product Specifications
Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse ab254015) is a panel of 4 rabbit recombinant monoclonal antibodies specific to fibroblast markers, used to identify fibroblasts which are connective tissue cells involved in extracellular matrix (ECM production, wound healing and tissue repair.
This panel contains antibodies to Vimentin (UniProt: P08670), Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) (UniProt: P62736 + P63267), Hsp47 (UniProt: P50454) and S100A4 (UniProt: P26447) in convenient trial sizes (20 µL) to allow for efficient and cost-effect testing of the antibodies simultaneously.
The individual antibodies included in this panel are:- Rabbit recombinant monoclonal anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)
- Rabbit recombinant monoclonal anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (ab32575)
- Rabbit recombinant monoclonal anti-Hsp47 antibody [EPR4217] (ab109117)
- Rabbit recombinant monoclonal anti-S100A4 antibody [EPR14639(2)] (ab197896)
All of these component antibodies are available for purchase individually using their corresponding abIDs.
Quality and Validation
Abcams high quality validation processes ensure each component antibody is sensitive and specific to the target protein.
All antibodies in this panel have been validated in human and mouse samples in a variety of applications, and some also have reactivity in other species.
For information on the species reactivity and application validation of each antibody within the panel, please consult the individual datasheet for each antibody. Suggested dilution factor and additional information on protocols can also be found on the antibody datasheets.
All antibodies in this panel have a minimum of 40 citations in peer-reviewed journals and are trusted by scientific community.
Related Products
Many of our antibody clones are also available as carrier-free formulations for easy conjugation to labels of your choice, or in pre-conjugated formats. Please search the clone name for alternative formulations and related products.Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
Immunocytochemistry/ Immunofluorescence - Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse (ab254015)
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 staining Vimentin in wild-type HAP1 cells (top panel) and VIM knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 at 0.5μg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunocytochemistry/ Immunofluorescence - Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse (ab254015)
Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) cells labeling alpha smooth muscle Actin with purified Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575 at 1/500 dilution (5.2 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse (ab254015)
Anti-Hsp47 antibody [EPR4217] ab109117 staining Hsp47 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse (ab254015)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse smooth muscle tissue labeling alpha smooth muscle Actin with purified Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575 at a dilution of 1/200. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody.
Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse (ab254015)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human smooth muscle tissue labeling alpha smooth muscle Actin with purified Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575 at a dilution of 1/200. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500)
Negative control using PBS instead of primary antibody.
Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse (ab254015)
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling S100A4 using Anti-S100A4 antibody [EPR14639(2)] ab197896 at 1/2000 dilution. Goat Anti-Rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as a secondary antibody at 1/500 dilution. Cells were counterstained with Hematoxylin.
Inset image: negative control obtained using PBS instead of Anti-S100A4 antibody [EPR14639(2)] ab197896 and secondary antibody only.
Note: Cytoplasm and nuclear staining on human gastric carcinoma tissue was observed.
Immunocytochemistry/ Immunofluorescence - Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse (ab254015)
Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labeling alpha smooth muscle Actin (green) with purified Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab32575 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, anti-Tubulin (mouse mAb) at 1/1000 followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Alexa Fluor®594 goat anti-mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).
For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) were used. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse primary antibody) was used followed by anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse (ab254015)
Immunohistochemical staining of paraffin embedded mouse kidney with purified Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 at a working dilution of 1/250. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse (ab254015)
Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Fibroblast Marker (Vimentin, alpha smooth muscle Actin, Hsp47, S100A4) Antibody Panel - Human, Mouse (ab254015)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: Hsp47 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: HepG2 whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Hsp47 antibody [EPR4217] (Anti-Hsp47 antibody [EPR4217] ab109117) observed at 46 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.Anti-Hsp47 antibody [EPR4217] ab109117 was shown to recognize Hsp47 in wild-type HAP1 cells as signal was lost at the expected MW in Hsp47 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Hsp47 knockout samples were subjected to SDS-PAGE. Anti-Hsp47 antibody [EPR4217] ab109117 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hsp47 antibody [EPR4217] (Anti-Hsp47 antibody [EPR4217] ab109117)
Predicted band size: 46 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com