AB252201

FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7)

Name: FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) | Abcam
Brand: Abcam Limited
SKU: AB252201

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(2 Publications)

FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) (ab252201) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) (AB252201)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) (AB252201)

Immunohistochemical analysis of Human Tonsil tissue labelling FOXP3 with ab99963.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) (AB252201)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for FOXP3 using ab243890 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) (AB252201)

IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human spleen* performed on a Leica BOND^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab191416, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

IHC-P

AbReview30757****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) (AB252201)

ab20034 staining FOXP3 in Cynomolgus Monkey Spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% donkey serum for 20 minutes at room temperature; antigen retrieval was by heat mediation using EDTA pH9.0. Samples were incubated with primary antibody (1/100 dilution) for 30 minutes. A Biotin-conjugated Donkey anti-mouse polyclonal (1/2000 dilution) was used as the secondary antibody.

This image was generated from the hybridoma version of the product.

Image is courtesy of an AbReview submitted by Jing Ma

Key facts

Reacts with

Human

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Product details

FOXP3 Antibody Panel ab252201 contains multiple trial-sized versions of anti-human antibody clones against FOXP3, specifically selected for high performance in IHC. This panel contains 4 recombinant rabbit monoclonal antibodies and 1 recombinant mouse monoclonal antibody against human FOXP3. They are provided in trial sizes to allow you to easily evaluate which is the best FOXP3 antibody for your IHC research assay needs.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [BLR034F] to FOXP3 (20 μL) ab243890

- Rabbit monoclonal [EPR15038-69] to FOXP3 (20 μL) ab191416

- Rabbit monoclonal [EPR22102-37] to FOXP3 (20 μL) ab215206

- Rabbit monoclonal [SP97] to FOXP3 (20 μL) ab99963

- Mouse monoclonal [236A/E7] to FOXP3 (20 μL) ab20034

Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab252201-1Kit", "assetComponentDetails": [ { "size":"2 x 10 µL", "name":"Anti-FOXP3 antibody [EPR22102-37]", "number":"AB252201-CMP03", "productcode":"AB215206" }, { "size":"2 x 10 µL", "name":"Anti-FOXP3 antibody [BLR034F]", "number":"AB252201-CMP01", "productcode":"AB243890" }, { "size":"2 x 10 µg", "name":"Anti-FOXP3 antibody [236A/E7]", "number":"AB252201-CMP02", "productcode":"AB20034" }, { "size":"2 x 10 µL", "name":"Anti-FOXP3 antibody [EPR15038-69]", "number":"AB252201-CMP04", "productcode":"AB191416" }, { "size":"2 x 10 µL", "name":"Anti-FOXP3 antibody [SP97]", "number":"AB252201-CMP05", "productcode":"AB99963" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.

Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.

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Product protocols

Visit the General protocols
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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

NPJ digital medicine 6:48 PubMed36959234

2023

Weakly supervised deep learning to predict recurrence in low-grade endometrial cancer from multiplexed immunofluorescence images.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Jiménez-Sánchez,Álvaro López-Janeiro,María Villalba-Esparza,Mikel Ariz,Ece Kadioglu,Ivan Masetto,Virginie Goubert,Maria D Lozano,Ignacio Melero,David Hardisson,Carlos Ortiz-de-Solórzano,Carlos E de Andrea

Journal of biochemical and molecular toxicology 36:e23115 PubMed35822655

2022

Cdc42 promotes thyroid cancer cell proliferation and migration and tumor-associated macrophage polarization through the PTEN/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Deyi Huang,Huali Qiu,Lin Miao,Lin Guo,Xiaoting Zhang,Mengmeng Lin,Zhongyun Li,Fang Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) (AB252201)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) (AB252201)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) (AB252201)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FoxP3 Antibody Panel (EPR15038-69, EPR22102-37, SP97, BLR034F, 236A/E7) (AB252201)

Key facts

Reacts with

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise