Hematopoietic Stem Cell Marker (CD34, CD59, CD90 / Thy1, CD38, c-Kit) Antibody Panel - Human (ab254022) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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- Frontiers in immunology 12:7960652021Rubella Virus Infected Macrophages and Neutrophils Define Patterns of Granulomatous Inflammation in Inborn and Acquired Errors of Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLudmila Perelygina,Raeesa Faisthalab,Emily Abernathy,Min-Hsin Chen,LiJuan Hao,Lionel Bercovitch,Diana K Bayer,Lenora M Noroski,Michael T Lam,Maria Pia Cicalese,Waleed Al-Herz,Arti Nanda,Joud Hajjar,Koen Vanden Driessche,Shari Schroven,Julie Leysen,Misha Rosenbach,Philipp Peters,Johannes Raedler,Michael H Albert,Roshini S Abraham,Hemalatha G Rangarjan,David Buchbinder,Lisa Kobrynski,Anne Pham-Huy,Julie Dhossche,Charlotte Cunningham Rundles,Anna K Meyer,Amy Theos,T Prescott Atkinson,Amy Musiek,Mehdi Adeli,Ute Derichs,Christoph Walz,Renate Krüger,Horst von Bernuth,Christoph Klein,Joseph Icenogle,Fabian Hauck,Kathleen E SullivanPubMed 35003119
Key facts
- Reactive species
- Human
- Target
- CD34
(See target data below)
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Target data
Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Additional Targets
CD59, THY1, CD38, KIT
Alternative names
CD34, Hematopoietic progenitor cell antigen CD34
What's included?
Recommended products
Hematopoietic Stem Cell Marker (CD34, CD59, CD90 / Thy1, CD38, c-Kit) Antibody Panel - Human (ab254022) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Reactive species
- Human
- Target
- CD34
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Hematopoietic Stem Cell Marker (CD34, CD59, CD90 / Thy1, CD38, c-Kit) Antibody Panel - Human ab254022 contains multiple trial-sized versions of anti-human antibody clones against CD34, CD59, CD90 / Thy1, CD38, c-Kit, specifically selected for high performance in various applications. This panel contains 5 recombinant rabbit monoclonal antibodies against human CD34, CD59, CD90 / Thy1, CD38, c-Kit. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EP373Y] to CD34 (20 μL) ab81289
- Rabbit monoclonal [EPR6425(2)] to CD59 (20 μL) ab133707
- Rabbit monoclonal [EPR3133] to CD90 / Thy1 (20 μL) ab133350
- Rabbit monoclonal [EPR4106] to CD38 (20 μL) ab108403
- Rabbit monoclonal [YR145] to c-Kit (20 μL) ab32363
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Western blot - Hematopoietic Stem Cell Marker (CD34, CD59, CD90 / Thy1, CD38, c-Kit) Antibody Panel - Human (ab254022)
Lane 1: Wild-type HAP1 whole cell lysate (40 μg)
Lane 2: CD59 knockout HAP1 whole cell lysate (40 μg)Lanes 1 - 2: Merged signal (red and green). Green - Anti-CD59 antibody [EPR6425(2)] (Anti-CD59 antibody [EPR6425(2)] ab133707) observed at 14 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-CD59 antibody [EPR6425(2)] ab133707 was shown to specifically react with CD59 in wild-type HAP1 cells as signal was lost in CD59 knockout cells. Wild-type and CD59 knockout samples were subjected to SDS-PAGE. Anti-CD59 antibody [EPR6425(2)] ab133707 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CD59 antibody [EPR6425(2)] (Anti-CD59 antibody [EPR6425(2)] ab133707)
Predicted band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hematopoietic Stem Cell Marker (CD34, CD59, CD90 / Thy1, CD38, c-Kit) Antibody Panel - Human (ab254022)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified Anti-CD38 antibody [EPR4106] ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Hematopoietic Stem Cell Marker (CD34, CD59, CD90 / Thy1, CD38, c-Kit) Antibody Panel - Human (ab254022)
All lanes : Anti-c-Kit antibody [YR145] (Anti-c-Kit antibody [YR145] ab32363) at 1/1000 dilutionLane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : KIT knockout HAP1 whole cell lysate
Lane 3 : HUVEC whole cell lysate
Lysates/proteins at 40 μg per lane.
Predicted band size: 110 kDaLanes 1 - 3: Merged signal (red and green). Green - Anti-c-Kit antibody [YR145] ab32363 observed at 109 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Lanes 1 - 3: Merged signal (red and green). Green - Anti-c-Kit antibody [YR145] ab32363 observed at 109 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-c-Kit antibody [YR145] ab32363 was shown to recognize 0 in wild-type HAP1 cells as signal was lost at the expected MW in KIT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KIT knockout samples were subjected to SDS-PAGE. Anti-c-Kit antibody [YR145] ab32363 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-c-Kit antibody [YR145] (Anti-c-Kit antibody [YR145] ab32363) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 40 µg
Lane 2: KIT knockout HAP1 whole cell lysate at 40 µg
Lane 3: HUVEC whole cell lysate at 40 µg
Predicted band size: 109 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hematopoietic Stem Cell Marker (CD34, CD59, CD90 / Thy1, CD38, c-Kit) Antibody Panel - Human (ab254022)
Immunohistochemical analysis of CD59 in paraffin embedded Human placenta tissue labelled with Anti-CD59 antibody [EPR6425(2)] ab133707 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Hematopoietic Stem Cell Marker (CD34, CD59, CD90 / Thy1, CD38, c-Kit) Antibody Panel - Human (ab254022)
Immunocytochemistry/Immunofluorescence analysis of HUVEC (Human umbilical vein endothelial cell line) cells labelling CD34 with purified Anti-CD34 antibody [EP373Y] ab81289 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain.
Control: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hematopoietic Stem Cell Marker (CD34, CD59, CD90 / Thy1, CD38, c-Kit) Antibody Panel - Human (ab254022)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human angiosarcoma labeling CD34 with unpurified Anti-CD34 antibody [EP373Y] ab81289 at 1/100-1/250.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hematopoietic Stem Cell Marker (CD34, CD59, CD90 / Thy1, CD38, c-Kit) Antibody Panel - Human (ab254022)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human seminoma tissue labelling c-Kit with unpurified Anti-c-Kit antibody [YR145] ab32363.
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