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Histone H3 (K4 methylation) Panel (mono methyl K4, di methyl K4, tri methyl K4) (ab103938) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
H31_HUMAN
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FD, H3C7, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, H3FI, HIST1H3E, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1
Histone H3 (K4 methylation) Panel (mono methyl K4, di methyl K4, tri methyl K4) (ab103938) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FD, H3C7, HIST1H3J, H3FJ, H3C12, HIST1H3I, H3FF, H3C11, HIST1H3H, H3FK, H3C10, HIST1H3G, H3FH, H3C8, HIST1H3F, H3FI, HIST1H3E, H3C6, HIST1H3D, H3FB, H3C4, HIST1H3C, H3FC, H3C3, HIST1H3B, H3FL, H3C2, HIST1H3A, H3FA, H3C1
H31_HUMAN
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Upon delivery aliquot
-20°C
ab103938 is a Histone H3 (K4 methylation) Panel designed for the validation and characterization of the methylation state of Histone H3 on K4. Methylation of the canonical core histones can contribute to the formation of transcriptionally active and inactive chromatin in response to various signalling pathways and is a central modification for regulating epigenetic transitions in chromatin. Methylation of Histone H3 K4 is associtated with euchromatin and active genes.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab8895 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and ALDOA (active) and MYO-D (inactive) promoters and over the y-Actin gene (active). Schematic diagram of the y-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.
ab8895 is specific for mono-methylated Lysine 4 of histone H3 and does not recognize di- or tri-methyl Lysine 4 nor methylation at Lysine 9. This is shown in lane 2 where the activity of the antibody is specifically blocked by the addition of the immunizing peptide (ab1340).
All lanes: Western blot - Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (AB8895) at 1/500 dilution
All lanes: Calf thymus histone lysate
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 18 kDa
Exposure time: 2min
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µl of ab32356 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab12209 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab1791 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
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