Histone H3 (K9 methylation) Panel (mono methyl K9, di methyl K9, tri methyl K9) (ab113754) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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- Nature communications 5:52642014Mitochondrial defects trigger proliferation of neighbouring cells via a senescence-associated secretory phenotype in Drosophila.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMai Nakamura et. al.PubMed 25345385
Key facts
- Target
- H3C1 di methyl K9
(See target data below)
Associated Products
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Target data
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Additional Targets
H3C1 methyl K9, H3C1 di methyl K9, H3C1 tri methyl K9
Alternative names
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, H3C1, H3FC, HIST1H3C, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K9me2
What's included?
Recommended products
Histone H3 (K9 methylation) Panel (mono methyl K9, di methyl K9, tri methyl K9) (ab113754) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Target
- H3C1 di methyl K9
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- -20°C
Notes
ab113754 is a Histone H3 (K9 methylation) Panel designed for the validation and characterization of the methylation state of Histone H3 on K9. Methylation of the canonical core histones can contribute to the formation of transcriptionally active and inactive chromatin in response to various signalling pathways and is a central modification for regulating epigenetic transitions in chromatin. Histone H3 methylation on K9 is a repressive modification, and H3 K9 tri-methylation has been shown to establish binding sites for heterochromatin protein 1.
designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Please Note: ab113754 is the replacement for ab103958.
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5 product images
ChIP - Histone H3 (K9 methylation) Panel (mono methyl K9, di methyl K9, tri methyl K9) (ab113754)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade ab1220 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first Kb of the transcribed region.
Western blot - Histone H3 (K9 methylation) Panel (mono methyl K9, di methyl K9, tri methyl K9) (ab113754)
Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab9045 shows significantly greater reactivity with mono methyl K9. This can be seen in lane 7, as the addition of Human Histone H3 (mono methyl K9) peptide ab1771 (mono methyl K9) completely blocks the activity of Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab9045. Weaker cross-reactivity is seen against mono methyl K27. This is shown in lane 3, as the addition of Human Histone H3 (mono methyl K27) peptide ab1780 only partially blocks the activity of Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab9045.
ChIP - Histone H3 (K9 methylation) Panel (mono methyl K9, di methyl K9, tri methyl K9) (ab113754)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade ab1791 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
ChIP - Histone H3 (K9 methylation) Panel (mono methyl K9, di methyl K9, tri methyl K9) (ab113754)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of Anti-Histone H3 (mono methyl K9) antibody - ChIP Grade ab9045 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
ChIP - Histone H3 (K9 methylation) Panel (mono methyl K9, di methyl K9, tri methyl K9) (ab113754)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade ab8898 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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