Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2) (ab238602) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2) (ab238602) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
ab238602 is a Human Embyonic Stem Cell Marker Panel containing 50μg of Oct4 rabbit polyclonal, 50μg of Tra-1-60 mouse monoclonal, 50μl of Nanog rabbit polyclonal antibody and 50μg of SOX2 rabbit polyclonal antibody.
The Human Embryonic Stem Cell Marker Panel is designed for the validation and characterization of cultured or newly derived human embryonic stem (ES) cell lines. The panel contains four antibodies to well known markers of human ES cells (Oct4, Nanog, SOX2 and Tra-1-60). Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique. Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).
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This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody Anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody Anti-TRA-1-60 (R) antibody [TRA-1-60] ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.
ICC/IF image of Sox2 (Anti-SOX2 antibody ab97959) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.01% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-SOX2 antibody ab97959, 1/500 dilution) overnight at +4°C.
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