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AB238602

Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2)

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(2 Publications)

Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2) (ab238602) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
2 Images
Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2) (AB238602)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2) (AB238602)

This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.

This image is courtesy of an anonymous collaborator.

Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2) (AB238602)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60 and SOX2) (AB238602)

ICC/IF image of Sox2 (ab97959) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.01% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97959, 1/500 dilution) overnight at +4°C.

This image is courtesy of an anonymous collaborator.

Key facts

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Complementary Products

Primary Antibodies

AB16288

Anti-TRA-1-60 (R) antibody [TRA-1-60]

Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (AB16288)

  • 4
  • 8
Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

  • 4
  • 75
Multiplex Kits

AB109884

Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4)

Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (AB109884)

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  • 1
Primary Antibodies

AB214549

Anti-Nanog antibody [EPR20694] - ChIP Grade

RabMAb|Advanced Validation|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Nanog antibody [EPR20694] - ChIP Grade (AB214549)

  • 5
  • 3
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Product details

ab238602 is a Human Embyonic Stem Cell Marker Panel containing 50μg of Oct4 rabbit polyclonal, 50μg of Tra-1-60 mouse monoclonal, 50μl of Nanog rabbit polyclonal antibody and 50μg of SOX2 rabbit polyclonal antibody.

The Human Embryonic Stem Cell Marker Panel is designed for the validation and characterization of cultured or newly derived human embryonic stem (ES) cell lines. The panel contains four antibodies to well known markers of human ES cells (Oct4, Nanog, SOX2 and Tra-1-60). Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique. Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).

designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

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What's included?

{ "values": { "1Unit": { "sellingSize": "1 Unit", "publicAssetCode":"ab238602-1Unit", "assetComponentDetails": [ { "size":"1 x 50 µg", "name":"Anti-Oct4 antibody - ChIP Grade", "number":"AB238602-CMP04", "productcode":"AB19857" }, { "size":"1 x 50 µg", "name":"Anti-SOX2 antibody", "number":"AB238602-CMP02", "productcode":"AB97959" }, { "size":"1 x 50 µg", "name":"Anti-TRA-1-60 (R) antibody [TRA-1-60]", "number":"AB238602-CMP03", "productcode":"AB16288" }, { "size":"1 x 50 µL", "name":"Anti-Nanog antibody - ChIP Grade", "number":"AB238602-CMP01", "productcode":"AB21624" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 10:949724 PubMed36091443

2022

Safety and biodistribution of exosomes derived from human induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhewei Gu,Zhiyu Yin,Pengbo Song,Ying Wu,Ying He,Maoshu Zhu,Zhengxin Wu,Sicheng Zhao,Hongri Huang,Huihuang Wang,Cailing Tong,Zhongquan Qi

Cells 11: PubMed35053384

2022

Generation of a -mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene Editing.

Applications

Unspecified application

Species

Unspecified reactive species

Parivash Nouri,Anja Zimmer,Stefanie Brüggemann,Robin Friedrich,Ralf Kühn,Nilima Prakash
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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