Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Key facts
- Reactive species
- Human
- Target
- H3-3A mutated G34W + G34V + G34R
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Target data
Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alternative names
H3.3A, H3F3, H3F3A, PP781, H3-3B, H3.3B, H3F3B, H3-3A, Histone H3.3
What's included?
Recommended products
Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Reactive species
- Human
- Target
- H3-3A mutated G34W + G34V + G34R
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel ab274410 contains multiple trial-sized versions of anti-human antibody clones against Histone H3.3 (Mutated G34W), Histone H3.3 (Mutated G34V) and Histone H3.3 (Mutated G34R), specifically selected for high performance in various applications. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EPR23581-39] to H3G34W (20 μL) ab272691
- Rabbit monoclonal [EPR23520-5] to H3G34V (20 μL) ab254401
- Rabbit monoclonal [EPR23519-91] to H3G34R (20 μL) ab254402
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
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Terms & Conditions.
20 product images
ChIP - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Chromatin was prepared from HEK-293T transfacted with Histone H3.3(mutated G34 R) and 293T transfacted with Histone H3.3(WT) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 2 μg of Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 (red), or 2 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 25 μl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci).
ChIP - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Chromatin was prepared from HEK-293T transfected with myc-His tagged Histone H3.3 mutated G34V and Histone H3.3 WT cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 2 μg of Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade ab254401 (red), or 2 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are located in the first kb of the transcribed region.
ChIP - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Chromatin was prepared from HEK-293T transfected with myc-His tagged Histone H3.3 mutated G34W and Histone H3.3 WT cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 2 μg of Anti-Histone H3.3 (mutated G34W) antibody [EPR23581-39] - ChIP Grade ab272691 (red), or 2 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are located in the first kb of the transcribed region.
Western blot - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
All lanes: Anti-Histone H3.3 (mutated G34 V) antibody [EPR23520-5] - ChIP Grade (Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade ab254401) at 1/1000 dilution.Lane 1: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34V expression vector containing a myc-His-tag®, whole cell lysate, 20 ug.
Lane 2: HEK-293T transfected with Histone H3.3 (WT) expression vector containing a myc-His-tag®, whole cell lysate, 20 ug.
Secondary (all lanes): Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051), 1/100000 dilution.
Predicted MW: 15 kDa.
Observed MW: 20 kDa.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Western blot - Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade (Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade ab254401) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34V expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with Histone H3.3 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Immunohistochemical analysis of paraffin-embedded human giant cell tumor of bone tissue labeling Histone H3.3 (mutated G34 R) with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on human giant cell tumor of bone (PMID: 29241742). The section was incubated with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Western blot - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
All lanes: Anti-Histone H3.3 (mutated G34 W) antibody [EPR23581-39] - ChIP Grade (Anti-Histone H3.3 (mutated G34W) antibody [EPR23581-39] - ChIP Grade ab272691) at 1/1000 dilution.Lane 1: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34W expression vector containing a myc-His-tag®, whole cell lysate, 40 ug.
Lane 2: HEK-293T transfected with Histone H3.3 (WT) expression vector containing a myc-His-tag®, whole cell lysate, 40 ug.
Secondary (all lanes): Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051), 1/50000 dilution.
Predicted MW: 15 kDa.
Observed MW: 20 kDa.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-Histone H3.3 (mutated G34W) antibody [EPR23581-39] - ChIP Grade (Anti-Histone H3.3 (mutated G34W) antibody [EPR23581-39] - ChIP Grade ab272691) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34W expression vector containing a myc-His-tag®, whole cell lysate at 40 µg
Lane 2: HEK-293T transfected with Histone H3.3 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 40 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 20 kDa
Flow Cytometry (Intracellular) - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc tagged Histone H3.3 WT construct (Left panel) and myc-tagged Histone H3.3 G34W construct (Right panel) cells labelling Histone H3.3(mutated G34 W) with Anti-Histone H3.3 (mutated G34W) antibody [EPR23581-39] - ChIP Grade ab272691 at 1/50 dilution (1μg). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
All lanes: Anti-Histone H3.3 (mutated G34 R) antibody [EPR23519-91] (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402) at 1/1000 dilution.Lane 1: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34R expression vector containing a myc-His-tag®, whole cell lysate, 20 ug.
Lane 2: HEK-293T transfected with Histone H3.3 (WT) expression vector containing a myc-His-tag®, whole cell lysate, 20 ug.
Lane 3: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34V expression vector containing a myc-His-tag®, whole cell lysate, 20 ug.
Lane 4: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34W expression vector containngi a myc-His-tag®, whole cell lysate, 20 ug.
Secondary (all lanes): Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051), 1/100000 dilution.
Predicted MW: 15 kDa
Observed MW: 20 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 seconds.
All lanes: Western blot - Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade (Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34R expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with Histone H3.3 (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 3: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34V expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 4: HEK-293T (human embryonic kidney) transfected with Histone H3.3 G34W expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 20 kDa
Flow Cytometry (Intracellular) - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc tagged Histone H3.3 WT construct (Left panel) and myc-tagged Histone H3.3 H3G34V construct (Right panel) cells labelling Histone H3.3(mutated G34 V) with Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade ab254401 at 1/500 dilution (0.1μg). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (Human embryonic kidney epithelial cell) (transfected with myc-tagged Histone H3.3 WT expression vector) (Left) or myc-tagged Histone H3.3 G34R expression vector (Right) cells labelling Histone H3.3 (mutated G34 R) with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 at 1/5000 dilution (0.01ug) (Both panels). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling Histone H3.3(mutated G34 W) with Anti-Histone H3.3 (mutated G34W) antibody [EPR23581-39] - ChIP Grade ab272691 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with Histone H3.3 G34W-Myc plasmid, while no staining in HEK-293T cells transfected with H3.3 WT -Myc plasmid. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 μg/ml dilution.
Immunocytochemistry/ Immunofluorescence - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling Histone H3.3(mutated G34 V) with Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade ab254401 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing nuclear staining in HEK-293T cells transfected with Histone H3.3 H3G34V-Myc plasmid, while no staining in HEK-293T cells transfected with H3.3 WT -Myc plasmid. Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 μg/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Immunohistochemical analysis of paraffin-embedded human giant cell tumor of bone tissue labeling Histone H3.3(mutated G34 W) with Anti-Histone H3.3 (mutated G34W) antibody [EPR23581-39] - ChIP Grade ab272691 at 1/250 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human giant cell tumor of bone (PMID: 29757500). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Immunohistochemical analysis of paraffin-embedded human giant cell tumor of bone tissue labeling Histone H3.3(mutated G34 V) with Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade ab254401 at 1/1000 dilution (0.542 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human giant cell tumor of bone. (PMID: 29241742). The section was incubated with Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade ab254401 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Dot Blot - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Dot blot analysis of Histone H3.3 (mutated G34 R) labeled with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 at 1/1000 dilution.
Lane 1: Histone H3.3 H3G34R peptide (aa28-40).
Lane 2: Histone H3.3 H3G34R peptide (aa26-36).
Lane 3: Histone H3.3 WT peptide (aa26-40).Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Immunohistochemical analysis of paraffin-embedded human chondroblastoma tissue labeling Histone H3.3(mutated G34 W) with Anti-Histone H3.3 (mutated G34W) antibody [EPR23581-39] - ChIP Grade ab272691 at 1/250 dilution followed by ready to use Goat Anti-Rabbit IgG H&L (HRP).
Negative control: No staining in human chondroblastoma (PMID: 29757500).
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Immunohistochemical analysis of paraffin-embedded human chondroblastoma tissue labeling Histone H3.3(mutated G34 V) with Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade ab254401 at 1/1000 dilution (0.542 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: No staining on human chondroblastoma (PMID: 29241742).
The section was incubated with Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade ab254401 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Histone H3.3 (mutated G34 R) with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human testis. The section was incubated with Anti-Histone H3.3 (mutated G34R) antibody [EPR23519-91] - ChIP Grade ab254402 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Dot Blot - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Dot blot analysis of Histone H3.3 (mutated G34 W) labeled with Anti-Histone H3.3 (mutated G34W) antibody [EPR23581-39] - ChIP Grade ab272691 at 1/1000 dilution.
Lane 1: Histone H3.3 H3G34W peptide (aa28-37).
Lane 2: Histone H3.3 H3G34W peptide (aa33-43).
Lane 3: Histone H3.3 H3G34W peptide (aa28-43).
Lane 4: Histone H3.3 WT peptide (aa28-43).Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Dot Blot - Human Histone H3.3 (Mutated G34W, G34V, G34R) Antibody Panel (ab274410)
Dot blot analysis of Histone H3.3 (mutated G34 V) labeled with Anti-Histone H3.3 (mutated G34V) antibody [EPR23520-5] - ChIP Grade ab254401 at 1/1000 dilution.
Lane 1: Histone H3.3 H3G34V peptide (aa28-40).
Lane 2: Histone H3.3 H3G34V peptide (aa26-38).
Lane 3: Histone H3.3 WT peptide (aa26-40).Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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