Immune Checkpoint Inhibitors Panel - Human IHC
- Recombinant
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Immune Checkpoint Inhibitors Panel - Human IHC (ab278174) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
View Alternative Names
CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, hPD-1, CD366, TIM3, TIMD3, HAVCR2, Hepatitis A virus cellular receptor 2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIMD-3, TIM-3, CD223, FDC, LAG3, Lymphocyte activation gene 3 protein, LAG-3, CD152, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1, VSIG9, VSTM3, TIGIT, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3, C10orf54, SISP1, VISTA, PP2135, UNQ730/PRO1412, VSIR, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, Stress-induced secreted protein-1, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor, Sisp-1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LAG-3 with ab209236 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining on immunocytes of human tonsil [PMID : 11527700; PMID : 16757686].
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for TIGIT using ab243903 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Formalin-fixed, paraffin-embedded human lymph node tissue stained for CTLA4 using ab237712 at 0.25 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CTLA4 using ab237712 at 0.25 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PD1 with ab137132 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA antigen retrieval solution, and microwave treatment for 20 min at 20% power. Anti-Rabbit HRP polymer was used as secondary antibody. Opal tyramide amplification was performed using Opal 540 fluorophore. Counterstained with DAPI stain.
Image scanned with Vectra 3.0 and analyzed via Inform software.
This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling TIM 3 with ab241332 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and membranous staining on Kupffer cells of human liver (PMID : 27192565) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling PD-L1 with ab228415 at 1/5000 dilution. The tissue was incubated with ab228415 at 4° overnight. followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Membranous and cytoplasmic staining in human placenta (PMID : 12538684) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Antigen retrieval : Universal HIER antigen retrieval reagent (10X) (ab208572).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CTLA4 with ab237712 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human tonsil is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.
The section was incubated with ab237712 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human placenta staining PD-L1 with ab228415 at 1/500 dilution. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10 mins. The section was incubated with ab228415 for 10 mins at room temperature. The secondary antibody used was ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained Hematoxylin. Performed on a Leica Biosystems BOND® RX instrument.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human T-cell lymphoma tissue labeling PD1 with unpurified ab137132 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling TIM 3 with ab241332 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and membranous staining on both infiltrated immunocytes and tumor cells of human lung cancer (PMID : 22586058) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lung carcinoma staining PD-L1 with ab228415 at 1/500 dilution. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10 mins. The section was incubated with ab228415 for 10 mins at room temperature. The secondary antibody used was ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained Hematoxylin. Performed on a Leica Biosystems BOND® RX instrument.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PD1 with purified ab137132 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control : Secondary antibody only using PBS instead of primary antibody. Counterstained with hematoxylin.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PD1 with unpurified ab137132 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Immune Checkpoint Inhibitors Panel - Human IHC (AB278174)
IHC image of ab228415 staining PD-L1 in human tonsil formalin fixed paraffin embedded tissue sections*, performed on a Leica BOND RX (standard Protocol F, Polymer Refine kit).
The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 30 mins at 98°C. The section was then incubated with ab228415, 0.06μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system for 8 minutes at room temperature. DAB was used as the chromogen for 10 minutes at room temperature. The section was then counterstained with hematoxylin, blued, dehydrated, cleared and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Complementary Products
Primary Antibodies
AB251600
Anti-GITR antibody [CAL52] - BSA and Azide free
BOND RX™ Validated|RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Anti-GITR antibody [CAL52] - BSA and Azide free (AB251600)](https://content.abcam.com/products/images/gitr-antibody-cal52-bsa-and-azide-free-ab251600--immunocytochemistry-immunofluorescence-img45133.jpg)
- 0
- 0
Primary Antibodies
AB256370
Anti-2B4 antibody [EPR23692-33]
BOND RX™ Validated|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-2B4 antibody [EPR23692-33] (AB256370)](https://content.abcam.com/products/images/2b4-antibody-epr23692-33-ab256370--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41353.jpg)
- 0
- 0
![Immunohistochemistry (Frozen sections) - Anti-PD1 antibody [NAT105] (AB52587)](https://content.abcam.com/products/images/pd1-antibody-nat105-ab52587--immunohistochemistry-frozen-sections-img439062.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR20261] (AB209236)](https://content.abcam.com/products/images/lag-3-antibody-epr20261-ab209236--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6541.jpg)
Product details
Immune Checkpoint Inhibitors Panel - Human IHC ab278174 contains multiple trial-sized versions of anti-human antibody clones against PD-L1, PD1, LAG3, TIM 3, VISTA, CTLA4 and TIGIT specifically selected for high performance in various applications. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [73-10] to PD-L1 (20 μL) ab228415
- Rabbit monoclonal [EPR4877(2)] to PD1 (20 μL) ab137132
- Rabbit monoclonal [EPR20261] to LAG-3 (20 μL) ab209236
- Rabbit monoclonal [EPR22241] to TIM 3 (20 μL) ab241332
- Rabbit monoclonal [EPR21050] to VISTA (20 μL) ab230950
- Rabbit monoclonal [CAL49] to CTLA4 (20 μL) ab237712
- Rabbit monoclonal [BLR047F] to TIGIT - BSA free (20 μL) ab243903
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
These immune checkpoint molecules regulate immune activation and tolerance to prevent overactive immune responses. They interact with specific ligands to transmit inhibitory signals that reduce the proliferation of T cells and diminish cytokine production. CTLA4 competes with CD28 to bind B7 molecules thereby inhibiting T cell activation. PD1 interaction with PD-L1 results in the suppression of T cell activity in tissues. These interactions are essential components of the immune system's self-tolerance mechanisms often working as part of larger protein complexes.
Pathways
Immune checkpoints play integral roles in the immune regulation pathways that prevent autoimmune reactions and maintain immune homeostasis. CTLA4 and PD1 are linked to the T cell receptor (TCR) signaling pathway inhibiting activation signals through interactions with their ligands. They relate closely to proteins like CD28 which has opposing effects on T cell activation. Their regulation involves complex signaling cascades including SHP2 and PI3K pathways which further control immune cell responses.
Target data
Additional targets
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com