AB252193

LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26)

Name: LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) | Abcam
Brand: Abcam Limited
SKU: AB252193
Price: 620 USD
Availability: InStock

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(1 Publication)

LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193) is part of our antibody panels range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Immunohistochemical analysis of paraffin-embedded human tonsil Hodgkin's lymphoma labeling Lymphocyte Activation Gene 3 with ab209236 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on immunocytes of the human Hodgkin's lymphoma [PMID : 11527700; PMID : 16757686].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using ab237718 at 0.3 μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Immunohistochemical analysis of paraffin embedded Human Hodgkins lymphoma tissue labeling LAG-3 with ab180187 at 1/1000.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using ab237719 at 0.3 μg/ml in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Formalin-fixed, paraffin-embedded Human Hodgkin's Lymphoma tissue stained for Lymphocyte Activation Gene 3 using ab227706 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using ab227706 at 1/100 dilution in immunohistochemical analysis.

Key facts

Reacts with

Human

Applications

IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"<p></p>" } } }

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Product details

Lymphocyte Activation Gene 3 Antibody Panel ab252193 contains multiple trial-sized versions of anti-human antibody clones against Lymphocyte Activation Gene 3, specifically selected for high performance in IHC. This panel contains 6 recombinant rabbit monoclonal antibodies against human Lymphocyte Activation Gene 3. They are provided in trial sizes to allow you to easily evaluate which is the best Lymphocyte Activation Gene 3 antibody for your IHC research assay needs.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [CAL25] to Lymphocyte Activation Gene 3 (20 μL) ab237718

- Rabbit monoclonal [CAL26] to Lymphocyte Activation Gene 3 (20 μL) ab237719

- Rabbit monoclonal [CAL77] to Lymphocyte Activation Gene 3 (20 μL) ab237720

- Rabbit monoclonal [EPR20261] to Lymphocyte Activation Gene 3 (20 μL) ab209236

- Rabbit monoclonal [EPR4392(2)] to Lymphocyte Activation Gene 3 - C-terminal (20 μL) ab180187

- Rabbit monoclonal [SP346] to Lymphocyte Activation Gene 3 - C-terminal (20 μL) ab227706

Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab252193-1Kit", "assetComponentDetails": [ { "size":"2 x 10 µL", "name":"Anti-Lymphocyte Activation Gene 3 antibody [EPR20261]", "number":"AB252193-CMP03", "productcode":"AB209236" }, { "size":"2 x 10 µL", "name":"Anti-Lymphocyte Activation Gene 3 antibody [CAL77]", "number":"AB252193-CMP06", "productcode":"AB237720" }, { "size":"2 x 10 µL", "name":"Anti-Lymphocyte Activation Gene 3 antibody [EPR4392(2)] - C-terminal", "number":"AB252193-CMP02", "productcode":"AB180187" }, { "size":"2 x 10 µL", "name":"Anti-Lymphocyte Activation Gene 3 antibody [CAL25]", "number":"AB252193-CMP04", "productcode":"AB237718" }, { "size":"2 x 10 µL", "name":"Anti-Lymphocyte Activation Gene 3 antibody [CAL26]", "number":"AB252193-CMP05", "productcode":"AB237719" }, { "size":"2 x 10 µL", "name":"Anti-Lymphocyte Activation Gene 3 antibody [SP346] - C-terminal", "number":"AB252193-CMP01", "productcode":"AB227706" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAG-3 also known as CD223 is a transmembrane protein with a mass of approximately 70 kDa. It acts as a checkpoint molecule similar to CTLA-4 and PD-1 in the immune system. LAG-3 is expressed on activated T cells natural killer cells and some dendritic cells. It interacts with its ligand MHC class II leading to inhibition of cellular proliferation and cytokine secretion an important part of immune response regulation.

Biological function summary

LAG-3 is involved in maintaining immune homeostasis and preventing autoimmunity. It is not part of a complex but works in synergy with other immune checkpoint molecules. When interacting with MHC class II LAG-3 transmits inhibitory signals that attenuate immune cell responses. Its role in immune regulation helps to balance immune activation and tolerance which is essential for preventing tissue damage while protecting against pathogens.

Pathways

The inhibitory role of LAG-3 integrates into the T cell receptor (TCR) signaling pathway and is also linked to the PD-1 signaling pathway. In TCR signaling LAG-3 negatively regulates T cell activation. LAG-3 is functionally related to proteins like PD-1 and CTLA-4 sharing similar roles in immune checkpoint pathways that are critical for maintaining immune balance.

LAG-3 has links to cancer and autoimmune diseases. In cancer LAG-3 expression on tumor-infiltrating lymphocytes correlates with immune evasion by tumors. This relationship appears in various cancers including melanoma and renal cell carcinoma. In autoimmune diseases LAG-3's expression on regulatory T cells helps control conditions such as multiple sclerosis. It collaborates with CTLA-4 in modulating immune responses that prevent excessive immune activation and tissue damage associated with autoimmunity.

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Product protocols

Visit the General protocols
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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 12:8015-8022 PubMed32943934

2020

Moxibustion Enhances Chemotherapy of Breast Cancer by Affecting Tumor Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Xue,Xingli Fu,Yin Zhu,Nili Da,Jianbin Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193)

Key facts

Reacts with

Applications

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise