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LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (AB252193), expandable thumbnail

Key facts

Reactive species

Human

Associated Products

Select an associated product type

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LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Key facts

Reactive species

Human

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Lymphocyte Activation Gene 3 Antibody Panel ab252193 contains multiple trial-sized versions of anti-human antibody clones against Lymphocyte Activation Gene 3, specifically selected for high performance in IHC. This panel contains 6 recombinant rabbit monoclonal antibodies against human Lymphocyte Activation Gene 3. They are provided in trial sizes to allow you to easily evaluate which is the best Lymphocyte Activation Gene 3 antibody for your IHC research assay needs.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [CAL25] to Lymphocyte Activation Gene 3 (20 μL) ab237718

- Rabbit monoclonal [CAL26] to Lymphocyte Activation Gene 3 (20 μL) ab237719

- Rabbit monoclonal [CAL77] to Lymphocyte Activation Gene 3 (20 μL) ab237720

- Rabbit monoclonal [EPR20261] to Lymphocyte Activation Gene 3 (20 μL) ab209236

- Rabbit monoclonal [EPR4392(2)] to Lymphocyte Activation Gene 3 - C-terminal (20 μL) ab180187

- Rabbit monoclonal [SP346] to Lymphocyte Activation Gene 3 - C-terminal (20 μL) ab227706


Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.


Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

LAG-3 also known as CD223 is a transmembrane protein with a mass of approximately 70 kDa. It acts as a checkpoint molecule similar to CTLA-4 and PD-1 in the immune system. LAG-3 is expressed on activated T cells natural killer cells and some dendritic cells. It interacts with its ligand MHC class II leading to inhibition of cellular proliferation and cytokine secretion an important part of immune response regulation.

Biological function summary

LAG-3 is involved in maintaining immune homeostasis and preventing autoimmunity. It is not part of a complex but works in synergy with other immune checkpoint molecules. When interacting with MHC class II LAG-3 transmits inhibitory signals that attenuate immune cell responses. Its role in immune regulation helps to balance immune activation and tolerance which is essential for preventing tissue damage while protecting against pathogens.

Pathways

The inhibitory role of LAG-3 integrates into the T cell receptor (TCR) signaling pathway and is also linked to the PD-1 signaling pathway. In TCR signaling LAG-3 negatively regulates T cell activation. LAG-3 is functionally related to proteins like PD-1 and CTLA-4 sharing similar roles in immune checkpoint pathways that are critical for maintaining immune balance.

Associated diseases and disorders

LAG-3 has links to cancer and autoimmune diseases. In cancer LAG-3 expression on tumor-infiltrating lymphocytes correlates with immune evasion by tumors. This relationship appears in various cancers including melanoma and renal cell carcinoma. In autoimmune diseases LAG-3's expression on regulatory T cells helps control conditions such as multiple sclerosis. It collaborates with CTLA-4 in modulating immune responses that prevent excessive immune activation and tissue damage associated with autoimmunity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193)

    Immunohistochemical analysis of paraffin embedded Human Hodgkins lymphoma tissue labeling LAG-3 with Anti-LAG-3 antibody [EPR4392(2)] - C-terminal ab180187 at 1/1000.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using Anti-LAG-3 antibody [SP346] - C-terminal ab227706 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193)

    Immunohistochemical analysis of paraffin-embedded human tonsil Hodgkin's lymphoma labeling Lymphocyte Activation Gene 3 with Anti-LAG-3 antibody [EPR20261] ab209236 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on immunocytes of the human Hodgkin's lymphoma [PMID: 11527700; PMID: 16757686].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using Anti-LAG-3 antibody [CAL25] ab237718 at 0.3 μg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LAG-3 Antibody Panel (EPR20261, EPR4392(2), SP346, CAL77, CAL25, CAL26) (ab252193)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lymphocyte Activation Gene 3 using Anti-LAG-3 antibody [CAL26] ab237719 at 0.3 μg/ml in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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