Macrophage Marker panel containing 5 antibodies against Human CD11b, CD68, CD163, CD14, CD16
- Provided as a sampler panel to allow you to easily evaluate each in your required applications.
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- Cancers 15:2023Polyploid Giant Cancer Cells Are Frequently Found in the Urine of Prostate Cancer Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Nalleli Garrido Castillo,Julien Anract,Nicolas Barry Delongchamps,Olivier Huillard,Fatima BenMohamed,Alessandra Decina,Thierry Lebret,Roger Dachez,Patrizia Paterlini-BréchotPubMed 37444476
- iScience 25:1039712022Coagulation factor V is a T-cell inhibitor expressed by leukocytes in COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Wang,Prasanti Kotagiri,Paul A Lyons,Rafia S Al-Lamki,Federica Mescia,Laura Bergamaschi,Lorinda Turner,Michael D Morgan,Fernando J Calero-Nieto,Karsten Bach,Nicole Mende,Nicola K Wilson,Emily R Watts,Patrick H Maxwell,Patrick F Chinnery,Nathalie Kingston,Sofia Papadia,Kathleen E Stirrups,Neil Walker,Ravindra K Gupta,David K Menon,Kieren Allinson,Sarah J Aitken,Mark Toshner,Michael P Weekes,James A Nathan,Sarah R Walmsley,Willem H Ouwehand,Mary Kasanicki,Berthold Göttgens,John C Marioni,Kenneth G C Smith,Jordan S Pober,John R BradleyPubMed 35224470
Key facts
- Reactive species
- Human
- Target
- ITGAM
(See target data below)
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Target data
Function
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:20008295, PubMed:9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
Additional Targets
CD68, ITGAM, CD68, CD163, CD14, FCGR3A, CD14, CD163, FCGR3A
Alternative names
CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor
What's included?
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Macrophage Marker panel containing 5 antibodies against Human CD11b, CD68, CD163, CD14, CD16
- Provided as a sampler panel to allow you to easily evaluate each in your required applications.
Key facts
- Reactive species
- Human
- Target
- ITGAM
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Product Specifications
Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013) is a panel of 6 rabbit recombinant monoclonal antibodies to macrophage markers. Macrophage markers are proteins used to identify and study macrophages, immune cells involved in phagocytosis, inflammation and tissue repair.
This panel contains antibodies to CD11b (UniProt: P11215), CD68 (UniProt: P34810), CD163 (UniProt: Q2VLH6), CD14 (UniProt: P08571) and CD16 (UniProt: P08637) in convenient trial sizes (20 µL) to allow for efficient and cost-effect testing of the antibodies simultaneously.
The individual antibodies included in this panel are:- Rabbit recombinant monoclonal anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
- Rabbit recombinant monoclonal anti-CD68 antibody [EPR20545] (ab213363)
- Rabbit recombinant monoclonal anti-CD163 antibody [EPR19518] (ab182422)
- Rabbit recombinant monoclonal anti-CD14 antibody [SP192] (ab183322)
- Rabbit recombinant monoclonal anti-CD16 antibody [SP175] (ab183354)
All of these component antibodies are available for purchase individually using their corresponding abIDs.
Quality and Validation
Abcams high quality validation processes ensure each component antibody is sensitive and specific to the target protein.
All antibodies in this panel have been validated in human samples in a variety of applications, and some also have reactivity in other species.
For information on the species reactivity and application validation of each antibody within the panel, please consult the individual datasheet for each antibody. Suggested dilution factor and additional information on protocols can also be found on the antibody datasheets.
All antibodies in this panel have a minimum of 50 citations in peer-reviewed journals and are trusted by scientific community.
Related Products
Many of our antibody clones are also available as carrier-free formulations for easy conjugation to labels of your choice, or in pre-conjugated formats. Please search the clone name for alternative formulations and related products.Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD11b CD14 CD16 CD68 and CD163 are important macrophage markers. CD11b also known as integrin alpha M has a mass of about 170 kDa is found on the surface of macrophages neutrophils and some T cells. It plays a mechanical role in cell adhesion migration and phagocytosis. CD14 acts as a co-receptor for the detection of bacterial lipopolysaccharides and is expressed on monocytes and macrophages. CD16 or FcγRIII is involved in antibody-dependent cellular cytotoxicity (ADCC) found on natural killer cells macrophages and some granulocytes. CD68 a marker of macrophage lineage is important in phagocytosis and is present in tissue macrophages. CD163 known for scavenging hemoglobin-haptoglobin complexes is expressed on monocytes and macrophages aiding in anti-inflammatory responses.
- Biological function summary
These macrophage markers participate in essential immune system functions. CD11b binds to iC3b opsonized particles facilitating phagocytosis and inflammatory signaling. CD14 acting with TLR4 triggers innate immune responses against pathogens. CD16 engages with the Fc region of IgG antibodies mediating ADCC. CD68 is part of the lysosomal/endosomal membrane associating with scavenger receptors in phagocytosis and tissue remodeling. CD163 modulates anti-inflammatory processes by binding to hemoglobin-haptoglobin complexes leading to the release of anti-inflammatory cytokines. These markers are vital to immune surveillance and the clearance of pathogens and debris.
- Pathways
CD11b and CD14 play roles in the Toll-like receptor (TLR) signaling pathway which is critical for initiating immune responses. CD11b interacts with complement component iC3b aiding in immune complex clearance. CD16 is involved in the FcγRIIB-mediated inhibitory pathway balancing immune activation. CD68 contributes to pathways involved in lysosomal degradation processes. CD163 is linked to the IL-10-mediated anti-inflammatory signaling pathway. These macrophage markers contribute to the coordination of host defense and inflammatory resolution.
- Associated diseases and disorders
CD11b is associated with chronic inflammatory diseases such as rheumatoid arthritis due to its role in leukocyte recruitment. CD14 has connections to sepsis as it participates in the recognition of bacterial LPS leading to systemic inflammation. CD16 contributes to autoimmune diseases; its dysfunction can lead to impaired ADCC. CD68 levels have been studied in atherosclerosis linking it to foam cell formation and plaque stability. CD163 with its anti-inflammatory properties relates to inflammatory disorders like multiple sclerosis linked to hemoglobin-haptoglobin clearance and IL-10 production. These markers offer potential therapeutic targets for treating these diseases.
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013)
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/8000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on macrophages of human cervical carcinoma is observed (PMID: 12118106). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical cancer tissue sections labeling CD11b with purified Anti-CD11b antibody [EP1345Y] - C-terminal ab52478 at 1:1000 dilution (0.28 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Hematoxylin was used as a counterstain.
Negative control: PBS instead of the primary antibody (inset).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD163 with Anti-CD163 antibody [EPR19518] ab182422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on Kupffer cells of human liver is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013)
Unpurified Anti-CD11b antibody [EP1345Y] - C-terminal ab52478 staining CD11b in the THP-1 (Human monocytic leukemia cell line) cell line by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with 100% methanol. Samples were incubated with primary antibody (1/250). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 was used as the secondary antibody (1/1000). Nuclei were stained with DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013)
Immunohistochemical analysis of paraffin embedded human liver tissue labeling CD16 with Anti-CD16 antibody [SP175] ab183354 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013)
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD14 with Anti-CD14 antibody [SP192] ab183322 at 1/100.
Immunocytochemistry/ Immunofluorescence - Macrophage Marker (CD11b, CD68, CD163, CD14, CD16) Antibody Panel - Human (ab254013)
Immunofluorescent analysis of 100% methanol-fixed U937 (human histiocytic lymphoma cell line) cells labeling CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on U937 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
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