Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (ab218257) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
RBFOX3, NEFH, DLG4, SYP
Microtubule-associated protein 2, MAP-2, MAP2
Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (ab218257) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel ab218257 contains recombinant rabbit monoclonal antibodies against MAP2, NeuN, Neurofilament heavy polypeptide, PSD95, and Synaptophysin.
Since mature neurons are no longer able to divide their purpose focuses on receiving, processing, and transferring information in the central and peripheral nervous systems.
The antibodies in this panel were selected for their exceptional performance in IHC, alongside other applications. Please see the individual datasheets for additional information.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Synaptophysin Neurofilament heavy polypeptide (NF-H) PSD95 MAP2 and NeuN are essential proteins in neuronal tissues. Synaptophysin a glycoprotein with a mass of approximately 38 kDa is expressed mainly in presynaptic vesicles. Neurofilament heavy polypeptide or NF-H is a component of the neuronal cytoskeleton with a mass of about 200 kDa found throughout neurons. PSD95 also called DLG4 is an important scaffold protein in synaptic junctions. MAP2 known for its expression in dendrites is vital for microtubule stabilization. NeuN also called Fox-3 is a well-known marker for mature neurons with high expression in the nuclei of post-mitotic neurons.
These proteins play important roles in neuronal development structure and function. Synaptophysin participates in synaptic vesicle cycling aiding neurotransmitter release. NF-H as part of the neurofilament complex contributes to axonal caliber and stability. PSD95 organizes protein complexes at postsynaptic sites facilitating synaptic signaling. MAP2 stabilizes microtubules essential for dendritic morphology and transport. NeuN marking indicates post-mitotic functionally mature neurons useful in identifying neuronal populations through immunohistochemistry.
These proteins integrate into critical signaling cascades in the nervous system. Synaptophysin and PSD95 play roles in synaptic transmission pathways influencing neurotransmitter release and postsynaptic receptor clustering. NF-H and MAP2 fit into the cytoskeletal dynamics pathway affecting neuronal branching and plasticity. NeuN serves as a neuronal marker particularly highlighting neurons in mature neural circuits. Through these pathways PSD95 often interacts with NMDA receptors while Synaptophysin forms complexes with SNARE proteins.
Alterations in these proteins link to several neurological conditions. Synaptophysin changes associate with Alzheimer's disease where synaptic dysfunction occurs. NF-H accumulation relates to amyotrophic lateral sclerosis (ALS) contributing to axonal degeneration. In both cases proteins such as MAPT (tau) and TDP-43 interplay with these alterations. Synaptic and cytoskeletal dysfunction in these diseases underlines the importance of biomarkers like NeuN and MAP2 in clinical research and diagnostics.
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Lanes 1 - 4: Merged signal (red and green). Green - Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker ab238135 observed at 75 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa. Secondary: Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776), 1/20000 dilution.
Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker ab238135 was shown to react with PSD95 in wild-type SH-SY5Y cells in Western blot with loss of signal observed in DLG4 knockout cell line Human PSD95 knockout SH-SY5Y cell line ab280043 (DLG4 knockout cell lysate Human PSD95 knockout SH-SY5Y cell lysate ab280102). Wild-type SH-SY5Y and DLG4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with Anti-PSD95 antibody [EPR23124-118] - Synaptic Marker ab238135 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse Cortex tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 at 1/100 dilution, followed by Goat anti-Rabbit IgG (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on neurons of mouse Cortex is observed. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 secondary antibody at 1/1000 dilution.
Immunofluorescent staining of PC-12 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified Anti-Synaptophysin antibody [YE269] - Synaptic Marker ab32127 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counter stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.
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