Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Synaptophysin Neurofilament heavy polypeptide (NF-H) PSD95 MAP2 and NeuN are essential proteins in neuronal tissues. Synaptophysin a glycoprotein with a mass of approximately 38 kDa is expressed mainly in presynaptic vesicles. Neurofilament heavy polypeptide or NF-H is a component of the neuronal cytoskeleton with a mass of about 200 kDa found throughout neurons. PSD95 also called DLG4 is an important scaffold protein in synaptic junctions. MAP2 known for its expression in dendrites is vital for microtubule stabilization. NeuN also called Fox-3 is a well-known marker for mature neurons with high expression in the nuclei of post-mitotic neurons.

Biological function summary

These proteins play important roles in neuronal development structure and function. Synaptophysin participates in synaptic vesicle cycling aiding neurotransmitter release. NF-H as part of the neurofilament complex contributes to axonal caliber and stability. PSD95 organizes protein complexes at postsynaptic sites facilitating synaptic signaling. MAP2 stabilizes microtubules essential for dendritic morphology and transport. NeuN marking indicates post-mitotic functionally mature neurons useful in identifying neuronal populations through immunohistochemistry.

Pathways

These proteins integrate into critical signaling cascades in the nervous system. Synaptophysin and PSD95 play roles in synaptic transmission pathways influencing neurotransmitter release and postsynaptic receptor clustering. NF-H and MAP2 fit into the cytoskeletal dynamics pathway affecting neuronal branching and plasticity. NeuN serves as a neuronal marker particularly highlighting neurons in mature neural circuits. Through these pathways PSD95 often interacts with NMDA receptors while Synaptophysin forms complexes with SNARE proteins.

Associated diseases and disorders

Alterations in these proteins link to several neurological conditions. Synaptophysin changes associate with Alzheimer's disease where synaptic dysfunction occurs. NF-H accumulation relates to amyotrophic lateral sclerosis (ALS) contributing to axonal degeneration. In both cases proteins such as MAPT (tau) and TDP-43 interplay with these alterations. Synaptic and cytoskeletal dysfunction in these diseases underlines the importance of biomarkers like NeuN and MAP2 in clinical research and diagnostics.