AB218257

Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (ab218257) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

View Alternative Names

Microtubule-associated protein 2, MAP-2, MAP2

3 Images

Immunocytochemistry/ Immunofluorescence - Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (AB218257)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (AB218257)

Immunofluorescent staining of PC-12 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab32127 at a dilution of 1/50. An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counter stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor^® 594 goat anti-mouse was used at a dilution of 1/500.

Immunohistochemistry (Frozen sections) - Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (AB218257)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (AB218257)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse Cortex tissue labeling MAP2 with ab183830 at 1/100 dilution, followed by Goat anti-Rabbit IgG (Alexa Fluor^®488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on neurons of mouse Cortex is observed. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

Supplier Data

Western blot - Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (AB218257)

Lane 1 : Wild-type SH-SY5Y cell lysate, 20 ug

Lane 2 : DLG4 knockout SH-SY5Y cell lysate, 20 ug

Lane 3 : HeLa cell lysate, 20 ug

Lane 4 : HL-60 cell lysate, 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab238135 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa. Secondary : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776), 1/20000 dilution.

ab238135 was shown to react with PSD95 in wild-type SH-SY5Y cells in Western blot with loss of signal observed in DLG4 knockout cell line ab280043 (DLG4 knockout cell lysate ab280102). Wild-type SH-SY5Y and DLG4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab238135 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

false

Key facts

Target

MAP2

target

style

left-column

Product details

Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel ab218257 contains recombinant rabbit monoclonal antibodies against MAP2, NeuN, Neurofilament heavy polypeptide, PSD95, and Synaptophysin.

Since mature neurons are no longer able to divide their purpose focuses on receiving, processing, and transferring information in the central and peripheral nervous systems.

The antibodies in this panel were selected for their exceptional performance in IHC, alongside other applications. Please see the individual datasheets for additional information.

Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

style

left-column

What's included?

{ "values": { "1Pack": { "sellingSize": "1 Pack", "publicAssetCode":"ab218257-1Pack", "assetComponentDetails": [ { "size":"2 x 10 µL", "name":"Anti-MAP2 antibody [EPR19691]", "number":"AB218257-CMP03", "productcode":"AB183830" }, { "size":"2 x 10 µL", "name":"Anti-Neurofilament heavy polypeptide antibody [EPR20020]", "number":"AB218257-CMP04", "productcode":"AB207176" }, { "size":"2 x 10 µL", "name":"Anti-NeuN antibody [EPR12763]", "number":"AB218257-CMP02", "productcode":"AB177487" }, { "size":"2 x 10 µL", "name":"Anti-Synaptophysin antibody [YE269]", "number":"AB218257-CMP01", "productcode":"AB32127" }, { "size":"2 x 10 µL", "name":"Anti-PSD95 antibody [EPR23124-118]", "number":"AB218257-CMP05", "productcode":"AB238135" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin Neurofilament heavy polypeptide (NF-H) PSD95 MAP2 and NeuN are essential proteins in neuronal tissues. Synaptophysin a glycoprotein with a mass of approximately 38 kDa is expressed mainly in presynaptic vesicles. Neurofilament heavy polypeptide or NF-H is a component of the neuronal cytoskeleton with a mass of about 200 kDa found throughout neurons. PSD95 also called DLG4 is an important scaffold protein in synaptic junctions. MAP2 known for its expression in dendrites is vital for microtubule stabilization. NeuN also called Fox-3 is a well-known marker for mature neurons with high expression in the nuclei of post-mitotic neurons.

Biological function summary

These proteins play important roles in neuronal development structure and function. Synaptophysin participates in synaptic vesicle cycling aiding neurotransmitter release. NF-H as part of the neurofilament complex contributes to axonal caliber and stability. PSD95 organizes protein complexes at postsynaptic sites facilitating synaptic signaling. MAP2 stabilizes microtubules essential for dendritic morphology and transport. NeuN marking indicates post-mitotic functionally mature neurons useful in identifying neuronal populations through immunohistochemistry.

Pathways

These proteins integrate into critical signaling cascades in the nervous system. Synaptophysin and PSD95 play roles in synaptic transmission pathways influencing neurotransmitter release and postsynaptic receptor clustering. NF-H and MAP2 fit into the cytoskeletal dynamics pathway affecting neuronal branching and plasticity. NeuN serves as a neuronal marker particularly highlighting neurons in mature neural circuits. Through these pathways PSD95 often interacts with NMDA receptors while Synaptophysin forms complexes with SNARE proteins.

Alterations in these proteins link to several neurological conditions. Synaptophysin changes associate with Alzheimer's disease where synaptic dysfunction occurs. NF-H accumulation relates to amyotrophic lateral sclerosis (ALS) contributing to axonal degeneration. In both cases proteins such as MAPT (tau) and TDP-43 interplay with these alterations. Synaptic and cytoskeletal dysfunction in these diseases underlines the importance of biomarkers like NeuN and MAP2 in clinical research and diagnostics.

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

See full target information MAP2

Additional targets

RBFOX3,NEFH,DLG4,SYP

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel

Immunocytochemistry/ Immunofluorescence - Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (AB218257)

Immunohistochemistry (Frozen sections) - Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (AB218257)

Western blot - Mature Neuron Marker (MAP2, NeuN, NFH, PSD95, Synaptophysin) Antibody Sampler Panel (AB218257)

Key facts

Target

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise