Mature Oligodendrocyte Marker panel containing 5 rabbit monoclonal antibodies against Human and Mouse Olig2, MBP, MOG, SOX10.
- Specifically selected for high performance in various applications.
- Provided as a sampler panel to allow you to easily evaluate each in your required applications.
Images
Publications
Filter by
- Acta neuropathologica communications 11:592023Upregulation of carbonic anhydrase 1 beneficial for depressive disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Cheng,Yue Wang,Yong He,Yu Tian,Junjie Li,Chong Chen,Xingzhe Xu,Zhonghao Wu,Heming Yu,Xiangyu Chen,Yili Wu,Weihong Song,Zhifang Dong,Huatai Xu,Peng XiePubMed 37013604
- Neurotoxicity research 41:41-562023Catalpol Mitigates Alzheimer's Disease Progression by Promoting the Expression of Neural Stem Cell Exosomes Released miR-138-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengxi Meng,Huize Chen,Chunjun Deng,Zeyu MengPubMed 36595161
- Neural regeneration research 15:894-9022019Rosmarinic acid ameliorates hypoxia/ischemia induced cognitive deficits and promotes remyelination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan Li,Miao-Miao Cui,Nwobodo Alexander Kenechukwu,Yi-Wei Gu,Yu-Lin Chen,Si-Jing Zhong,Yu-Ting Gao,Xue-Yan Cao,Li Wang,Fu-Min Liu,Xiang-Ru WenPubMed 31719255
Key facts
- Reactive species
- Mouse, Human
- Target
- OLIG2
(See target data below)
Abcam Recommends
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
Additional Targets
MBP, SOX10, MBP, MOG, SOX10, OLIG2
Alternative names
BHLHB1, BHLHE19, PRKCBP2, RACK17, OLIG2, Oligodendrocyte transcription factor 2, Oligo2, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, bHLHb1, bHLHe19
What's included?
Recommended products
Mature Oligodendrocyte Marker panel containing 5 rabbit monoclonal antibodies against Human and Mouse Olig2, MBP, MOG, SOX10.
- Specifically selected for high performance in various applications.
- Provided as a sampler panel to allow you to easily evaluate each in your required applications.
Key facts
- Reactive species
- Mouse, Human
- Target
- OLIG2
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Product Specifications
Mature Oligodendrocyte Marker (Olig2, MBP, MOG, SOX10) Antibody Sampler Panel - Human, Mouse (ab254026) is a panel of 5 rabbit recombinant monoclonal antibodies specific to markers of mature oligodendrocytes. Mature oligodendrocytes are responsible for producing myelin, which insulates neuronal axons in the central nervous system (CNS).
This panel contains antibodies to Olig2 (UniProt: Q13516), Myelin Basic Protein (UniProt: P04370), Myelin oligodendrocyte glycoprotein (UniProt: Q16653) and SOX10 (UniProt: P56693) in convenient trial sizes (20 µL) to allow for efficient and cost-effect testing of the antibodies simultaneously.
The individual antibodies included in this panel are:- Rabbit recombinant monoclonal anti-Olig2 antibody [EPR2673] (ab109186)
- Rabbit recombinant monoclonal anti-Myelin Basic Protein antibody [EPR21188] (ab218011)
- Rabbit recombinant monoclonal anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)
- Rabbit recombinant monoclonal anti-SOX10 antibody [EPR4007] (ab155279)
- Rabbit recombinant monoclonal anti-SOX10 antibody [SP267] (ab227680)
All of these component antibodies are available for purchase individually using their corresponding abIDs.
Quality and Validation
Abcams high quality validation processes ensure each component antibody is sensitive and specific to the target protein.
All antibodies in this panel have been validated in human, mouse and rat samples in a variety of applications, and some also have reactivity in other species.
For information on the species reactivity and application validation of each antibody within the panel, please consult the individual datasheet for each antibody. Suggested dilution factor and additional information on protocols can also be found on the antibody datasheets.
Related Products
Many of our antibody clones are also available as carrier-free formulations for easy conjugation to labels of your choice, or in pre-conjugated formats. Please search the clone name for alternative formulations and related products.Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myelin Basic Protein (MBP) is a critical component of the myelin sheath surrounding nerve fibers. It is also known as mbr or myelin oligodendrocyte. MBP is found in the central nervous system primarily expressed in oligodendrocytes where it plays a structural role in compaction of the myelin membrane. The molecular mass of human MBP commonly ranges from 18.5 kDa to 21.5 kDa depending on the isoform. Alongside MBP SOX10 and Olig2 are significant oligodendrocyte markers that play roles in the development and maintenance of oligodendrocyte identity and function. Myelin oligodendrocyte glycoprotein (MOG) further complements these proteins in stabilizing the myelin sheath.
- Biological function summary
MBP and its associated proteins are essential for maintaining the stability and integrity of myelin in the central nervous system. These proteins ensure proper nerve signal transmission. While MBP is not part of a larger protein complex it interacts closely with lipids in the myelin membrane to ensure proper sheath formation. SOX10 functions as a transcription factor that is critical for the regulation of myelin genes while Olig2 is involved in the differentiation and maturation of oligodendrocytes serving as an olig2 marker and playing a pivotal role in oligodendrocyte development. These markers are used in olig2 staining for detecting oligodendrocytes.
- Pathways
MBP SOX10 Olig2 and MOG play significant roles within the myelination pathway vital for establishing and maintaining proper neural connections. MBP interacts closely with other proteins involved in myelination such as myelin-associated glycoprotein (MAG) ensuring the stability and functionality of the neuronal environment. The Notch signaling pathway also engages with SOX10 and Olig2 influencing oligodendrocyte differentiation and maturation which highlights their role as oligodendrocyte markers.
- Associated diseases and disorders
MBP SOX10 Olig2 and MOG are significantly linked to multiple sclerosis (MS) and other demyelinating diseases. MS involves the immune system's attack on oligodendrocyte markers leading to degradation of the myelin sheath. MBP is often targeted in autoimmune reactions that occur in MS. Additionally congenital defects in the expression of SOX10 or Olig2 can contribute to severe myelination disorders such as leukodystrophies. The connection between these proteins and neural pathologies highlights their importance in maintaining neurological health.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
Immunohistochemistry (Frozen sections) - Mature Oligodendrocyte Marker (Olig2, MBP, MOG, SOX10) Antibody Sampler Panel - Human, Mouse (ab254026)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain (cerebrum) tissue labeling Myelin Basic Protein with Anti-Myelin Basic Protein antibody [EPR21188] ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Positive staining in the axonal fiber tracts on rat cerebrum tissue section (PMID: 23144976) is observed. The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Perform heat-mediated antigen retrieval by using ab94681 (Tris-EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mature Oligodendrocyte Marker (Olig2, MBP, MOG, SOX10) Antibody Sampler Panel - Human, Mouse (ab254026)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue sections labeling SOX10 with Anti-SOX10 antibody [SP267] ab227680 at 1/100 dilution (0.25 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the human melanoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-SOX10 antibody [SP267] ab227680 for 10 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mature Oligodendrocyte Marker (Olig2, MBP, MOG, SOX10) Antibody Sampler Panel - Human, Mouse (ab254026)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with Purified Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mature Oligodendrocyte Marker (Olig2, MBP, MOG, SOX10) Antibody Sampler Panel - Human, Mouse (ab254026)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Myelin oligodendrocyte glycoprotein with purified Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mature Oligodendrocyte Marker (Olig2, MBP, MOG, SOX10) Antibody Sampler Panel - Human, Mouse (ab254026)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse breast tissue sections labeling SOX10 with Anti-SOX10 antibody [SP267] ab227680 at 1/100 dilution (0.25 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the mouse breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-SOX10 antibody [SP267] ab227680 for 10 mins at room temperature.
Immunocytochemistry/ Immunofluorescence - Mature Oligodendrocyte Marker (Olig2, MBP, MOG, SOX10) Antibody Sampler Panel - Human, Mouse (ab254026)
Immunocytochemistry/ Immunofluorescence analysis of B16-F0 ( mouse melanoma epithelial cell-like) cells labeling SOX10 with purified Anti-SOX10 antibody [SP267] ab227680 at 1:25 (3 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mature Oligodendrocyte Marker (Olig2, MBP, MOG, SOX10) Antibody Sampler Panel - Human, Mouse (ab254026)
Immunohistochemical staining of paraffin embedded human cerebral cortex with purified Anti-Olig2 antibody [EPR2673] ab109186 at a working dilution of 1/100. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mature Oligodendrocyte Marker (Olig2, MBP, MOG, SOX10) Antibody Sampler Panel - Human, Mouse (ab254026)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Myelin Basic Protein with Anti-Myelin Basic Protein antibody [EPR21188] ab218011 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human cerebrum (PMID: 22496821) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunocytochemistry/ Immunofluorescence - Mature Oligodendrocyte Marker (Olig2, MBP, MOG, SOX10) Antibody Sampler Panel - Human, Mouse (ab254026)
Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling SOX10 with purified Anti-SOX10 antibody [SP267] ab227680 at 1:25 (3 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com