Mouse Loading Control Antibody Panel (ab199713) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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- International journal of molecular sciences 24:2023Suppression of Indoxyl Sulfate Accumulation Reduces Renal Fibrosis in Sulfotransferase 1a1-Deficient Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuixian Hou,Mai Horikawa,Yuki Narita,Hirofumi Jono,Yutaka Kakizoe,Yuichiro Izumi,Takashige Kuwabara,Masashi Mukoyama,Hideyuki SaitoPubMed 37511089
- PloS one 9:e1083362014Large-scaled metabolic profiling of human dermal fibroblasts derived from pseudoxanthoma elasticum patients and healthy controls.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatricia Kuzaj et. al.PubMed 25265166
- Neuron 84:63-772014Deficiency in LRP6-mediated Wnt signaling contributes to synaptic abnormalities and amyloid pathology in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChia-Chen Liu et. al.PubMed 25242217
- Physiological reports 2:2014Adiponectin is not required for exercise training-induced improvements in glucose and insulin tolerance in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIan R W Ritchie et. al.PubMed 25214523
- Genes to cells : devoted to molecular & cellular mechanisms 19:704-212014Formation of nuclear bodies by the lncRNA Gomafu-associating proteins Celf3 and SF1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkira Ishizuka,Yuko Hasegawa,Kentaro Ishida,Kaori Yanaka,Shinichi NakagawaPubMed 25145264
- International journal of molecular sciences 15:14427-412014Low-frequency high-magnitude mechanical strain of articular chondrocytes activates p38 MAPK and induces phenotypic changes associated with osteoarthritis and pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerek H Rosenzweig et. al.PubMed 25196344
- PloS one 9:e1030442014The relationship between muscle fiber type-specific PGC-1α content and mitochondrial content varies between rodent models and humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGilles Gouspillou et. al.PubMed 25121500
- BMC cancer 14:5652014Constitutive AKT activation in follicular lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOuardia I Yahiaoui et. al.PubMed 25096023
- Frontiers in genetics 5:2562014X-inactivation normalizes O-GlcNAc transferase levels and generates an O-GlcNAc-depleted Barr body.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStéphanie Olivier-Van Stichelen et. al.PubMed 25136351
- Frontiers in aging neuroscience 6:1982014Subtype and regional-specific neuroinflammation in sporadic creutzfeldt-jakob disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFranc Llorens et. al.PubMed 25136317
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Mouse Loading Control Antibody Panel (ab199713) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Ab199713 is a sampler pack of loading control mouse antibodies and a Goat Anti-Mouse IgG secondary antibody conjugated to HRP.
This panel contains sample sizes of all mouse host primary antibodies against the following housekeeping targets: alpha Tubulin, GAPDH, COXIV, Histone H3, alpha 1 NaK ATPase.The Mouse Loading Control Antibody Panel is designed for validation and confirmation of western blot analysis when tested in conjunction with your proteins of interest.
Please refer to each individual component datasheet for storage instructions.
designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.Refer to the 'Associated Products' section below to see HRP-conjugated versions of these antibodies.
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We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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5 product images
Western blot - Mouse Loading Control Antibody Panel (ab199713)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406
All lanes: Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Lane 3: PC12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa, 50 kDa
Observed band size: 50 kDa
Exposure time: 150s
Western blot - Mouse Loading Control Antibody Panel (ab199713)
The membrane 1-5 was blocked in 5% Milk (1 hour). The membrane 6-10 was blocked in 5% BSA (1 hour). Abcam routinely uses 5% BSA to block however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent and significantly decreases the signal on the membrane. We have informed all users of the antibody of this finding.
All lanes: Western blot - Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484) at 1/1000 dilution
Lanes 1 and 6: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
Lanes 2 and 7: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lanes 3 and 8: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
Lanes 4 and 9: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 20 µg
Lanes 5 and 10: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 20 µg
SecondaryAll lanes: Goat anti-Mouse (HRP conjugated) at 1/5000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa
Western blot - Mouse Loading Control Antibody Panel (ab199713)
All lanes: Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab33985) at 1 µg/mL
Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Lane 4: Skeletal Muscle (Mouse) Tissue Lysate at 10 µg
Lane 5: Kidney (Cow) Tissue Lysate (ab29073) at 10 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 15 kDa
Exposure time: 1min
Western blot - Mouse Loading Control Antibody Panel (ab199713)
All lanes: Western blot - Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade ab24834) at 1 µg/mL
Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 2: Testis (Mouse) Tissue Lysate at 10 µg
Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 4: Testis (Rat) Tissue Lysate - normal tissue (ab29388) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 18 kDa
Exposure time: 3min
Western blot - Mouse Loading Control Antibody Panel (ab199713)
Lanes 1 - 3: Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free ab237969) at 1 µg/mL
Lanes 4 - 6: Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free ab237969) at 2 µg/mL
Lanes 7 - 9: Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free ab237969) at 5 µg/mL
Lanes 1, 4 and 7: ab29466 at 20 µg
Lanes 2, 5 and 8: ab27253 at 20 µg
Lanes 3, 6 and 9: ab7942 at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 113 kDa
Exposure time: 3min
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