Mouse Loading Control Antibody Panel (Alexa Fluor® 790) (ab200701) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Mouse Loading Control Antibody Panel (Alexa Fluor® 790) (ab200701) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
ab200701 is a sampler pack of loading control antibodies conjugated to Alexa Fluor® 790.
This panel contains sample sizes of primary antibodies against the following housekeeping targets: alpha Tubulin, GAPDH, and beta Actin.
The Mouse Loading Control Antibody Panel (Alexa Fluor® 790) is designed for validation and confirmation of western blot analysis when tested in conjunction with your proteins of interest.
designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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3 product images
Western blot - Mouse Loading Control Antibody Panel (Alexa Fluor® 790) (ab200701)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control ab184577 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.
All lanes: Western blot - Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control ab184577) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
Lane 4: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Predicted band size: 50 kDa
Observed band size: 52 kDa
Western blot - Mouse Loading Control Antibody Panel (Alexa Fluor® 790) (ab200701)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Alexa Fluor® 790 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184578 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.
All lanes: Western blot - Alexa Fluor® 790 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (Alexa Fluor® 790 Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab184578) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Predicted band size: 122 kDa, 36 kDa
Observed band size: 150 kDa, 37 kDa
Western blot - Mouse Loading Control Antibody Panel (Alexa Fluor® 790) (ab200701)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Alexa Fluor® 790 Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab184576 overnight at 4°C. Antibody binding was detected after washing to remove excess antibody and imaged using the Licor Odyssey CLx.
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