Natural Killer NK Cell Marker (CD3, NCAM1, CD94, NKG2D, SLAMF6, NK-p44) Antibody Panel - Human (ab254014) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Key facts
- Reactive species
- Human
- Target
- CD3D
(See target data below)
Associated Products
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Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).
Additional Targets
NCAM1, KLRD1, KLRK1, SLAMF6, NCR2
Alternative names
CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain
What's included?
Recommended products
Natural Killer NK Cell Marker (CD3, NCAM1, CD94, NKG2D, SLAMF6, NK-p44) Antibody Panel - Human (ab254014) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Reactive species
- Human
- Target
- CD3D
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Natural Killer NK Cell Marker (CD3, NCAM1, CD94, NKG2D, SLAMF6, NK-p44) Antibody Panel - Human ab254014 contains multiple trial-sized versions of anti-human antibody clones against CD3, NCAM1, CD94, NKG2D, SLAMF6, NK-p44, specifically selected for high performance in various applications. This panel contains 6 recombinant rabbit monoclonal antibodies against human CD3, NCAM1, CD94, NKG2D, SLAMF6, NK-p44. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [SP162] to CD3 (20 μL) ab135372
- Rabbit monoclonal [EP2567Y] to NCAM1 (20 μL) ab75813
- Rabbit monoclonal [EPR21003] to CD94 (20 μL) ab235441
- Rabbit monoclonal [EPR22935-131] to NKG2D (20 μL) ab233557
- Rabbit monoclonal [EPR22170] to SLAMF6 (20 μL) ab224201
- Rabbit monoclonal [EPR5060(2)] to NK-p44 (20 μL) ab133668
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Natural Killer NK Cell Marker (CD3, NCAM1, CD94, NKG2D, SLAMF6, NK-p44) Antibody Panel - Human (ab254014)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD3 with Anti-CD3 antibody [SP162] ab135372 at a 1/150 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Natural Killer NK Cell Marker (CD3, NCAM1, CD94, NKG2D, SLAMF6, NK-p44) Antibody Panel - Human (ab254014)
Immunohistochemical analysis of paraffin-embedded human NK/T-cell lymphoma tissue labeling CD94 with Anti-CD94 antibody [EPR21003] ab235441 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human NK/T-cell lymphoma (PMID: 11253136, PMID: 10828054, PMID: 12816864) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunocytochemistry/ Immunofluorescence - Natural Killer NK Cell Marker (CD3, NCAM1, CD94, NKG2D, SLAMF6, NK-p44) Antibody Panel - Human (ab254014)
Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling CD3 with purified Anti-CD3 antibody [SP162] ab135372 at 1/10 (10 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®; 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor®; 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control
Immunocytochemistry/ Immunofluorescence - Natural Killer NK Cell Marker (CD3, NCAM1, CD94, NKG2D, SLAMF6, NK-p44) Antibody Panel - Human (ab254014)
Immunofluorescent analysis of K562 cells labelling NK-p44 with Anti-NK-p44 antibody [EPR5060(2)] ab133668 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Natural Killer NK Cell Marker (CD3, NCAM1, CD94, NKG2D, SLAMF6, NK-p44) Antibody Panel - Human (ab254014)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (human Burkitt's lymphoma cell line) cells labeling SLAMF6 with Anti-SLAMF6 antibody [EPR22170] ab224201 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in the Ramos cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
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