Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Key facts
- Reactive species
- Human
- Target
- CXCR4
(See target data below)
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Target data
Function
Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation (PubMed:10452968, PubMed:18799424, PubMed:24912431, PubMed:28978524). Involved in the AKT signaling cascade (PubMed:24912431). Plays a role in regulation of cell migration, e.g. during wound healing (PubMed:28978524). Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels (PubMed:20228059). Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205). Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival (By similarity). (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) for human immunodeficiency virus-1/HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus (PubMed:10074122, PubMed:10756055, PubMed:8849450, PubMed:8929542, PubMed:9427609).
Additional Targets
NES, PDGFRA phospho Y572 + Y574, SOX2, VIM, DCX
Alternative names
CD184, C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, LESTR, LAP-3, LPS-associated protein 3, SDF-1 receptor, CXCR4
What's included?
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Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Reactive species
- Human
- Target
- CXCR4
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel ab263461 contains multiple trial-sized versions of anti-human antibody clones against CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin, specifically selected for high performance in various applications. This panel contains 6 recombinant rabbit monoclonal antibodies against human CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [UMB2] to CXCR4 (20 μL) ab124824
- Rabbit monoclonal [SP103] to Nestin (20 μL) ab105389
- Rabbit monoclonal [EPR22059-270] to PDGFR alpha (20 μL) ab203491
- Rabbit monoclonal [EPR3131] to SOX2 (20 μL) ab92494
- Rabbit monoclonal [EPR3776] to Vimentin - Cytoskeleton Marker (20 μL) ab92547
- Rabbit monoclonal [EPR19997] to Doublecortin (20 μL) ab207175
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
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10 product images
Immunocytochemistry/ Immunofluorescence - Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461)
ICC/IF image of Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with purified Anti-SOX2 antibody [EPR3131] ab92494 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461)
Confocal image showing nuclear staining on NCCIT cells
Anti-SOX2 antibody [EPR3131] ab92494 staining SOX2 in NCCIT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (1/1000) was used as the secondary antibody. Counterstained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (1/1000), Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.
Negative control 1 Anti-SOX2 antibody [EPR3131] ab92494 was used as the primary antibody at 1/200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 was used as the secondary at 1/1000.
Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 was used as the secondary at 1/1000.
Immunocytochemistry/ Immunofluorescence - Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-204 (human muscle rhabdomyosarcoma cell line) cells and A-172 (human brain glioblastoma cell line) labeling PDGFR alpha with Anti-PDGFR alpha antibody [EPR22059-270] ab203491 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membranous and cytoplasmic staining in A-204 cell line.
Negative control: A-172 (PMID:8425771.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461)
Immunofluorescence staining of Jurkat cells with purified Anti-CXCR4 antibody [UMB2] ab124824 at a working dilution of 1 in 250, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) . The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified Anti-CXCR4 antibody [UMB2] ab124824 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (ab15007) were used.
Immunocytochemistry/ Immunofluorescence - Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461)
Immunofluorescent analysis of 100% Methanol-fixed SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling Doublecortin with Anti-Doublecortin antibody [EPR19997] ab207175 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461)
Immunohistochemical staining of paraffin embedded human bladder cancer with purified Anti-CXCR4 antibody [UMB2] ab124824 at a working dilution of 1/500. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461)
Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461)
Formalin-fixed, paraffin-embedded human kidney tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Neural Progenitor Cell Marker (CXCR4, Nestin, PDGFR, SOX2, Vimentin, Doublecortin) Antibody Panel (ab263461)
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 staining Vimentin in wild-type HAP1 cells (top panel) and VIM knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 at 0.5μg/ml and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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