Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Key facts
- Reactive species
- Human
- Target
- NES
(See target data below)
Associated Products
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Target data
Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Additional Targets
SOX2, OCLN, CDH1, HES1, NOTCH1
Alternative names
Nbla00170, NES, Nestin
What's included?
Recommended products
Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
- Reactive species
- Human
- Target
- NES
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Neural Stem Cell (Neuroepithelial) Marker Antibody Panel - Human ab254027 contains multiple trial-sized versions of anti-human antibody clones against Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1 specifically selected for high performance in various applications. This panel contains 6 recombinant rabbit monoclonal antibodies against human Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [SP103] to Nestin (20 μL) ab105389
- Rabbit monoclonal [EPR3131] to SOX2 (20 μL) ab92494
- Rabbit monoclonal [EPR20992] to Occludin (20 μL) ab216327
- Rabbit monoclonal [EP700Y] to E Cadherin - Intercellular Junction Marker (20 μL) ab40772
- Rabbit monoclonal [EPR4226] to Hes1 (20 μL) ab108937
- Rabbit monoclonal [EP1238Y] to Notch1 (20 μL) ab52627
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
E-cadherin also known as cadherin-1 is a 120 kDa transmembrane protein important for cell-cell adhesion in epithelial tissues. It is primarily expressed in epithelial cells where it maintains cell structure and polarity. E-cadherin functions as an adhesion molecule in adherens junctions helping cells to bind tightly together. Nestin a marker of neural stem cells is an intermediate filament protein involved in cytoskeletal organisation with expression in neuronal precursor cells and certain adult stem cells. SOX2 a transcription factor weighing approximately 34 kDa plays a vital role in the regulation of embryonic stem cell pluripotency and neural progenitor cell maintenance. Notch1 Hes1 and Occludin each contribute to various physiological mechanisms. Notch1 a receptor around 300 kDa helps in cell fate determination while Hes1 serves as a transcriptional repressor in the Notch signaling pathway. Occludin about 65 kDa acts as an important player in forming tight junctions in epithelial and endothelial cells.
- Biological function summary
E-cadherin participates in maintaining the integrity and function of epithelial tissues. As part of the cadherin-catenin complex it connects the actin cytoskeleton to intercellular junctions creating a stable cell structure. Nestin contributes to cellular structural changes during neural development responding to environmental cues related to cell division and differentiation. SOX2's function centers around gene regulation necessary for sustaining pluripotency in multipotent cell populations. Notch1's involvement in cell differentiation signals within tissues with Hes1 functioning as part of feedback loops modulates downstream gene expression essential for developmental processes. Occludin forms part of the complex regulating paracellular permeability important for maintaining selective cellular barriers.
- Pathways
E-cadherin plays significant roles within the Wnt signaling pathway influencing processes related to cell fate and embryogenesis. This pathway also involves critical interactions with beta-catenin which stabilizes cell adhesion structures. Nestin engages in pathways related to neurogenesis guiding the specification and proliferation of neural stem cells. SOX2 is deeply integrated within both the pluripotency network and the Notch signaling pathway which cross-talks with other transcriptional regulators. Notch1 is an important component of the Notch signaling pathway affecting adjacent interactions between neighboring cells and often intersecting with Hes1 to influence gene expression patterns. Occludin participates in epithelial-mesenchymal transition pathways influencing cellular movement and plasticity.
- Associated diseases and disorders
Defects or dysregulation of E-cadherin are associated with cancer metastasis particularly in gastric and breast cancers where E-cadherin loss contributes to epithelial-mesenchymal transition. Nestin meanwhile provides markers for brain tumors and its abnormal expression links to certain aggressive cancer types. SOX2 has implications in ocular disorders notably those impairing vision due to developmental defects. Notch1 has links to T-cell acute lymphoblastic leukemia acting in concert with Hes1 to influence leukemogenesis. Occludin shows connection to inflammatory diseases like inflammatory bowel disease where its dysfunction may compromise the intestinal barrier.
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13 product images
Western blot - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
All lanes : Anti-Nestin antibody [SP103] (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389) at 1/100 dilutionLane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : NES knockout HAP1 whole cell lysate
Lysates/proteins at 20 μg per lane.
Predicted band size: 177 kDa
Lanes 1 - 2: Merged signal (red and green). Green - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 observed at 300 kDa. Red - loading control, ab18058, observed at 117 kDa.
Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 was shown to specifically react with NES in wild-type HAP1 cells as signal was lost in NES knockout cells. Wild-type and NES knockout samples were subjected to SDS-PAGE. Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Nestin antibody [SP103] - Neural Stem Cell Marker (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389) at 1/100 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: NES knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 177 kDa
Immunocytochemistry/ Immunofluorescence - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
ICC/IF image of Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Western blot - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
All lanes : Anti-Occludin antibody [EPR20992] (Anti-Occludin antibody [EPR20992] ab216327) at 1/1000 dilutionLane 1 : Wild-type HAP1 whole cell lysate at 40 μg
Lane 2 : OCLN (Occludin) knockout HAP1 whole cell lysate at 40 μg
Lane 3 : HeLa whole cell lysate (Low Occludin expression) at 20 μg
Lane 4 : HepG2 whole cell lysate lysate (High Occludin expression) at 20 μg
Predicted band size: 59 kDa
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Occludin antibody [EPR20992] ab216327 observed at 59 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-Occludin antibody [EPR20992] ab216327 was shown to recognize Occludin in wild-type HAP1 cells as signal was lost at the expected MW in OCLN (Occludin) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and OCLN (Occludin) knockout samples were subjected to SDS-PAGE. Anti-Occludin antibody [EPR20992] ab216327 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Occludin expression in HeLa is expected to be negative.All lanes: Western blot - Anti-Occludin antibody [EPR20992] (Anti-Occludin antibody [EPR20992] ab216327) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 40 µg
Lane 2: OCLN (Occludin) knockout HAP1 whole cell lysate at 40 µg
Lane 3: HeLa whole cell lysate (Low Occludin expression) at 20 µg
Lane 4: HepG2 whole cell lysate lysate (High Occludin expression) at 20 µg
Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
Immunohistochemical staining of paraffin-embedded human brain with purified Anti-Notch1 antibody [EP1238Y] ab52627 at a dilution of 1/150.
A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
Formalin-fixed, paraffin-embedded human endometrium tissue stained for Nestin using Anti-Nestin antibody [SP103] - Neural Stem Cell Marker ab105389 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on human colon is observed (PMID: 24268521). Counter stained with Hematoxylin.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB ab209101 (Rabbit specific IHC polymer detection kit HRP/DAB).
Immunocytochemistry/ Immunofluorescence - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
Confocal image showing nuclear staining on NCCIT cells
Anti-SOX2 antibody [EPR3131] ab92494 staining SOX2 in NCCIT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (1/1000) was used as the secondary antibody. Counterstained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (1/1000), Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.
Negative control 1 Anti-SOX2 antibody [EPR3131] ab92494 was used as the primary antibody at 1/200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 was used as the secondary at 1/1000.
Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 was used as the secondary at 1/1000.
Immunocytochemistry/ Immunofluorescence - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker ab40772 staining E Cadherin in HT-29 (Human colorectal adenocarcinoma) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Samples were incubated with primary antibody at 1/500 dilution. An Alexa Fluor® 488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 dilution. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution was used as a counterstain. DAPI was used as a nuclear counterstain. This is a confocal image showing membranous staining on HT-29 cell line.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
Primary ab concentration dilution: 1:200, (0.5ug/ml), Secondary ab: ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG, Secondary ab concentration: Prediluted, Tissue: Human brain, Fixative: Paraffin-embedded sections, Counter stain: Hematoxylin Antigen retrieval: Perform heat mediated antigen retrieval using Tris/EDTA Buffer, PH9
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with unpurified Anti-SOX2 antibody [EPR3131] ab92494 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells fixed with 4% PFA using purified Anti-Notch1 antibody [EP1238Y] ab52627 at a dilution of 1/150.
An Alexa Fluor® 555 goat anti-rabbit was used as the secondary and the sample was stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 after Anti-Notch1 antibody [EP1238Y] ab52627 as the negative control and is shown in the bottom right hand panel.
Immunocytochemistry/ Immunofluorescence - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells labeling Occludin with Anti-Occludin antibody [EPR20992] ab216327 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membrane staining on Caco-2 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Human (ab254027)
Formalin/PFA-fixed paraffin-embedded human colonic adenocarcinoma tissue stained for E Cadherin with unpurified Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker ab40772 at a 1/500 dilution in immunohistochemical analysis.
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