Neural Stem Cell Marker (Nestin, SOX2, Occludin, E Cadherin, Hes1, Notch1) Antibody Panel - Mouse

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

E-cadherin also known as cadherin-1 is a 120 kDa transmembrane protein important for cell-cell adhesion in epithelial tissues. It is primarily expressed in epithelial cells where it maintains cell structure and polarity. E-cadherin functions as an adhesion molecule in adherens junctions helping cells to bind tightly together. Nestin a marker of neural stem cells is an intermediate filament protein involved in cytoskeletal organisation with expression in neuronal precursor cells and certain adult stem cells. SOX2 a transcription factor weighing approximately 34 kDa plays a vital role in the regulation of embryonic stem cell pluripotency and neural progenitor cell maintenance. Notch1 Hes1 and Occludin each contribute to various physiological mechanisms. Notch1 a receptor around 300 kDa helps in cell fate determination while Hes1 serves as a transcriptional repressor in the Notch signaling pathway. Occludin about 65 kDa acts as an important player in forming tight junctions in epithelial and endothelial cells.

Biological function summary

E-cadherin participates in maintaining the integrity and function of epithelial tissues. As part of the cadherin-catenin complex it connects the actin cytoskeleton to intercellular junctions creating a stable cell structure. Nestin contributes to cellular structural changes during neural development responding to environmental cues related to cell division and differentiation. SOX2's function centers around gene regulation necessary for sustaining pluripotency in multipotent cell populations. Notch1's involvement in cell differentiation signals within tissues with Hes1 functioning as part of feedback loops modulates downstream gene expression essential for developmental processes. Occludin forms part of the complex regulating paracellular permeability important for maintaining selective cellular barriers.

Pathways

E-cadherin plays significant roles within the Wnt signaling pathway influencing processes related to cell fate and embryogenesis. This pathway also involves critical interactions with beta-catenin which stabilizes cell adhesion structures. Nestin engages in pathways related to neurogenesis guiding the specification and proliferation of neural stem cells. SOX2 is deeply integrated within both the pluripotency network and the Notch signaling pathway which cross-talks with other transcriptional regulators. Notch1 is an important component of the Notch signaling pathway affecting adjacent interactions between neighboring cells and often intersecting with Hes1 to influence gene expression patterns. Occludin participates in epithelial-mesenchymal transition pathways influencing cellular movement and plasticity.

Associated diseases and disorders

Defects or dysregulation of E-cadherin are associated with cancer metastasis particularly in gastric and breast cancers where E-cadherin loss contributes to epithelial-mesenchymal transition. Nestin meanwhile provides markers for brain tumors and its abnormal expression links to certain aggressive cancer types. SOX2 has implications in ocular disorders notably those impairing vision due to developmental defects. Notch1 has links to T-cell acute lymphoblastic leukemia acting in concert with Hes1 to influence leukemogenesis. Occludin shows connection to inflammatory diseases like inflammatory bowel disease where its dysfunction may compromise the intestinal barrier.