Neutrophil Marker panel containing 3 rabbit monoclonal antibodies against Human CD11b, CD16, CD55 and 2 mouse monoclonal antibodies against Human CD32, CD44.
- Specifically selected for high performance in various applications.
- Provided as a sampler panel to allow you to easily evaluate each in your required applications.
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Key facts
- Reactive species
- Human
- Target
- CD44
(See target data below)
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Target data
Function
Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).
Additional Targets
FCGR3A, FCGR2A, CD55
Alternative names
CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I
What's included?
Recommended products
Neutrophil Marker panel containing 3 rabbit monoclonal antibodies against Human CD11b, CD16, CD55 and 2 mouse monoclonal antibodies against Human CD32, CD44.
- Specifically selected for high performance in various applications.
- Provided as a sampler panel to allow you to easily evaluate each in your required applications.
Key facts
- Reactive species
- Human
- Target
- CD44
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Product Specifications
Neutrophil Marker (CD11b, CD16, CD32, CD44, CD55) Antibody Panel - Human (ab254016) is a panel of 5 monoclonal antibodies specific to neutrophil markers. Neutrophil markers are proteins used to identify and study neutrophils, which are key immune cells involved in inflammation, infection response, and tissue repair.
This panel contains 3 rabbit recombinant monoclonal antibodies and 2 mouse monoclonal antibodies to CD11b (UniProt: P11215), CD16 (UniProt: P08637), CD32A + CD32B (UniProt: P12318), CD44 (UniProt: P16070) and CD55 (UniProt: P08174) in convenient trial sizes (20 µg/ 20 µL) to allow for efficient and cost-effect testing of the antibodies simultaneously.
The individual antibodies included in this panel are:- Rabbit recombinant monoclonal anti-CD11b antibody [SP330] - C-terminal (ab224805)
- Rabbit recombinant monoclonal anti-CD16 antibody [SP175] (ab183354)
- Mouse monoclonal anti-CD32B + CD32A antibody [AT10] (ab23336)
- Mouse monoclonal anti-CD44 antibody [F10-44-2] (ab6124)
- Rabbit recombinant monoclonal anti-CD55 antibody [EPR6689] (ab133684)
All of these component antibodies are available for purchase individually using their corresponding abIDs.
Quality and Validation
Abcams high quality validation processes ensure each component antibody is sensitive and specific to the target protein.
All antibodies in this panel have been validated in human samples in a variety of applications, and some also have reactivity in other species.
For information on the species reactivity and application validation of each antibody within the panel, please consult the individual datasheet for each antibody. Suggested dilution factor and additional information on protocols can also be found on the antibody datasheets.
Related Products
Many of our antibody clones are also available as carrier-free formulations for easy conjugation to labels of your choice, or in pre-conjugated formats. Please search the clone name for alternative formulations and related products.Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Immunocytochemistry/ Immunofluorescence - Neutrophil Marker (CD11b, CD16, CD32, CD44, CD55) Antibody Panel - Human (ab254016)
ab6412 staining CD44 in A431 cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with Anti-Nestin antibody [Rat-401] - Neural Stem Cell Marker ab6142 at 5ugml then detected with an Alexa Fluor® 488 goat anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neutrophil Marker (CD11b, CD16, CD32, CD44, CD55) Antibody Panel - Human (ab254016)
Immunohistochemical analysis of paraffin embedded human spleen tissue labeling CD16 with Anti-CD16 antibody [SP175] ab183354 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neutrophil Marker (CD11b, CD16, CD32, CD44, CD55) Antibody Panel - Human (ab254016)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD11b using Anti-CD11b antibody [SP330] - C-terminal ab224805 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neutrophil Marker (CD11b, CD16, CD32, CD44, CD55) Antibody Panel - Human (ab254016)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling CD55 with Anti-CD55 antibody [EPR6689] ab133684 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Neutrophil Marker (CD11b, CD16, CD32, CD44, CD55) Antibody Panel - Human (ab254016)
IHC image of CD44 staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-CD44 antibody [F10-44-2] ab6124, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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