ab219089 is a sampler panel consisting of thoroughly-validated recombinant monoclonal antibodies against several variants of p53.
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ab219089 is a sampler panel consisting of thoroughly-validated recombinant monoclonal antibodies against several variants of p53.
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- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
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ab219089 is a sampler panel consisting of thoroughly-validated recombinant monoclonal antibodies against several variants of p53. This panel includes trial sizes of antibodies to unmodified p53, mutant p53, p53 (acetylated Lysine 382), p53 (phosphorylated Serine 20), p53 (phosphorylated Serine 392), p53 (phosphorylated Serine 46), and two anti-rabbit IgG (HRP) and anti-mouse IgG (HRP) antibodies.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
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We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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6 product images
Western blot - p53 Antibody Sampler Panel (ab219089)
All lanes : Anti-p53 (phospho S20) antibody [EPR2156(2)] (Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454) at 1/1000 dilution
Lane 1 : T47D (human mammary gland ductal carcinoma) treated with Etoposide whole cell lysate
Lane 2 : Untreated T47D (human mammary gland ductal carcinoma) whole cell lysate
Lysates/proteins at 10 μg per lane.
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size : 44 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minuteBlocking & dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-p53 (phospho S20) antibody [EPR2156(2)] (Anti-p53 (phospho S20) antibody [EPR2156(2)] ab157454) at 1/1000 dilution
Lane 1: T-47D (human mammary gland ductal carcinoma) treated with Etoposide whole cell lysate at 10 µg
Lane 2: Untreated T47D (human mammary gland ductal carcinoma) whole cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa
Exposure time: 1min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p53 Antibody Sampler Panel (ab219089)
Anti-p53 antibody [Y5] ab32049 showing positive staining in urinary bladder carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p53 Antibody Sampler Panel (ab219089)
IHC image of Anti-p53 (acetyl K382) antibody [EPR358(2)] ab75754 staining p53 (acetyl K382) in human breast adenocarcinoma formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-p53 (acetyl K382) antibody [EPR358(2)] ab75754, 1μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - p53 Antibody Sampler Panel (ab219089)
All lanes : Anti-p53 (phospho S46) antibody [EP42Y] (Anti-p53 (phospho S46) antibody [EP42Y] ab76242) at 1/5000 dilution
Lane 1 : Untreated HepG2 whole cell lysate
Lane 2 : HepG2 whole cell lysate, treated with etoposide
Lane 3 : HepG2 whole cell lysate, treated with etoposide, then the membrane was treated with alkaline phosphatase
Lysates/proteins at 10 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Exposure time : 1 minuteBlocking buffer: 2% BSA/TBST
Dilution buffer: 2% BSA/TBSTAll lanes: Western blot - Anti-p53 (phospho S46) antibody [EP42Y] (Anti-p53 (phospho S46) antibody [EP42Y] ab76242) at 1/5000 dilution
Lane 1: Untreated HepG2 whole cell lysate at 10 µg
Lane 2: HepG2 whole cell lysate, treated with etoposide at 10 µg
Lane 3: HepG2 whole cell lysate, treated with etoposide, then the membrane was treated with alkaline phosphatase at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa
Exposure time: 1min
Western blot - p53 Antibody Sampler Panel (ab219089)
Predicted band size : 43.6 kDaLanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 μg)
Lanes 2, 6 and 10: p53 knockout HAP1 cell lysate (20 μg)
Lanes 3, 7 and 11: A431 cell lysate (20 μg)
Lanes 4, 8 and 12: Saos-2 cell lysate (20 μg)
Lanes 1, 2, 3 and 4: Green signal from target – Anti-p53 antibody [DO-1] - ChIP Grade ab1101 observed at 53 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalAnti-p53 antibody [DO-1] (Anti-p53 antibody [DO-1] - ChIP Grade ab1101) was shown to specifically react with p53 when p53 knockout samples were used. Wild-type and p53 knockout samples were subjected to SDS-PAGE. Anti-p53 antibody [DO-1] - ChIP Grade ab1101 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - p53 Antibody Sampler Panel (ab219089)
Anti-p53 (phospho S392) antibody [EP155Y] ab33889, at a 1/100 dilution, staining p53 in paraffin embedded human prostate adenocarcinoma tissue by Immunohistochemistry.
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