Parkinson's Disease Research Antibody Panel (ab226767) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Parkinson's Disease Research Antibody Panel (ab226767) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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- Shipped at conditions
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- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
ab226767 is a Parkinson's Disease research panel containing 5 recombinant rabbit monoclonal antibodies and 1 Anti-Rabbit IgG (HRP) secondary antibody: Anti-alpha synuclein antibody, Anti-LRRK2 antibody, Anti-PINK1 antibody, Anti-PARK/DJ1 antibody and Goat Anti-Rabbit IgG (H&L) (HRP).
The hallmark pathologies of Parkinson's disease include the loss of dopaminergic neurons from the pars compacta, a portion of the substantia nigra in the midbrain, causing slowness of movement (bradykinesia), resting tremor and rigidity. This is coupled with the presence of intracellular protein aggregates known as Lewy bodies.
The antibodies in this panel were selected for their exceptional performance in imaging applications, alongside other applications. Please see the individual datasheets for additional information.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Parkinson's Disease Research Antibody Panel (ab226767)
IHC image of alpha Synuclein staining in normal Human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-Alpha-synuclein antibody [MJFR1] ab138501, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Parkinson's Disease Research Antibody Panel (ab226767)
Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma)(+/- treatment with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours) cells labeling PINK1 with Anti-PINK1 antibody [EPR20730] ab216144 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells treated with 10μM carbonyl cyanide 3-chlorophenylhydrazone (CCCP) for 24 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
The negative controls are as follows:
-ve control: PBS, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Parkinson's Disease Research Antibody Panel (ab226767)
Anti-PARK7/DJ1 antibody [EP2815Y] ab76008, at 1/250 dilution, staining PARK7/DJ1 in human brain by immunohistochemistry using paraffin-embedded tissue.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com