AB269812

PD-L1 / PD1 Multiplex IHC-IF Antibody Panel (PD-L1, PD1, CD68, CD3, Ki67, panCK)

Name: PD-L1 / PD1 Multiplex IHC-IF Antibody Panel (PD-L1, PD1, CD68, CD3, Ki67, panCK) (ab269812) | Abcam
Brand: Abcam Limited
SKU: AB269812
Price: 2295 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

PD-L1 / PD1 Multiplex IHC-IF Antibody Panel (PD-L1, PD1, CD68, CD3, Ki67, panCK) (ab269812) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

View Alternative Names

CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1

1 Images

Multiplex immunohistochemistry - PD-L1 / PD1 Multiplex IHC-IF Antibody Panel (PD-L1, PD1, CD68, CD3, Ki67, panCK) (AB269812)

mIHC

Supplier Data

Multiplex immunohistochemistry - PD-L1 / PD1 Multiplex IHC-IF Antibody Panel (PD-L1, PD1, CD68, CD3, Ki67, panCK) (AB269812)

Fluorescence multiplex immunohistochemical analysis of normal human tonsil tissue (formalin-fixed paraffin-embedded section).

Merged staining of anti-PD1 (ab237728; orange; Opal™520) anti-PDL1 (ab237726; green; Opal™540) anti-CD68 (ab192847; yellow; Opal™570) anti-CD3 epsilon (ab16669; red; Opal™620) anti-Ki67 (ab16667; light blue; Opal™650) and anti-PanCK (ab7753; grey; Opal™690).

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 7-color automation IHC kit (NEL821001KT Akoya Biosciences^®).

The section was incubated in six rounds of staining; in the order of ab237728 (1/500 dilution), ab237726 (1/500 dilution), ab192847 (1/300 dilution), ab16669 (1/300 dilution), ab16667 (1/200 dilution) and ab7753 (1/200 dilution); each using a separate fluorescent tyramide signal amplification system.

Sodium citrate antigen retrieval (Leica ER1 pH6.0 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round to avoid any cross-reactivity.

DAPI (dark blue) was used as a nuclear counter stain.

Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra Polaris.

Key facts

Reacts with

Human

Target

CD274

target

Applications

mIHC

applications

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "mIHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

PD-L1 / PD1 Multiplex IHC-IF Antibody Panel (PD-L1, PD1, CD68, CD3, Ki67, panCK) is specifically designed and validated for multiplex analysis of the tumor microenvironment (TME) using tyramide signal amplification (TSA) on human formalin fixed paraffin-embedded tissue (FFPE) sections. The order of staining and antibody dilutions have been optimized on human tonsil FFPE tissue.

This panel empowers researchers to explore the spatial interactions of these important immuno-oncology biomarkers, immune checkpoint proteins and immune cell-phenotyping markers within the tumor micro-environment. It allows for the 6 targets to be fluorescently detected on the same tissue section, plus a nuclear counterstain (DAPI).

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 7-color automation IHC kit (NEL821001KT, Akoya Biosciences®). Please refer to this kit's protocol for directions on how to perform the immunostaining.

The panel contains enough reagents for a minimum of 50 slides.

Panel contents, recommended order of staining and dilutions:

1. Recombinant PD1 antibody [CAL20] ab237728 (50 μl)

1/500 dilution; Opal™520

2. Recombinant PD-L1 antibody [CAL10] ab237726 (50 μl)

1/500 dilution; Opal™540

3. Recombinant CD68 antibody [SP251] ab192847 - Macrophage marker (50 μl)

1/300 dilution; Opal™570

4. CD3 antibody [SP7] ab16669 - T cell marker (50 μl)

1/300 dilution; Opal™620

5. Recombinant Ki67 antibody [SP6] ab16667 - Proliferation marker (50 μl)

1/200 dilution; Opal™650

6. Pan-cytokeratin antibody [C-11] ab7753 - Epithelial tissue marker (50 μg)

1/200 dilution; Opal™690

style

left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab269812-1Kit", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"Recombinant Anti-CD68 antibody [SP251]", "number":"AB269812-CMP04", "productcode":"AB192847" }, { "size":"1 x 50 µL", "name":"Anti-CD3 antibody [SP7]", "number":"AB269812-CMP01", "productcode":"AB16669" }, { "size":"1 x 50 µg", "name":"Anti-pan Cytokeratin antibody [C-11]", "number":"AB269812-CMP02", "productcode":"AB7753" }, { "size":"1 x 50 µL", "name":"Recombinant Anti-PD1 antibody [CAL20]", "number":"AB269812-CMP06", "productcode":"AB237728" }, { "size":"1 x 50 µL", "name":"Recombinant Anti-Ki67 antibody [SP6]", "number":"AB269812-CMP03", "productcode":"AB16667" }, { "size":"1 x 50 µL", "name":"Recombinant Anti-PD-L1 antibody [CAL10]", "number":"AB269812-CMP05", "productcode":"AB237726" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed : 10581077). Can also act as a transcription coactivator : in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed : 32929201).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28813410, PubMed : 28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

See full target information CD274

Additional targets

PDCD1,CD68,CD3E,MKI67,KRT76

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:27 PubMed39786598

2025

MUC5B regulates alterations in the immune microenvironment in nasopharyngeal carcinoma via the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongming Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PD-L1 / PD1 Multiplex IHC-IF Antibody Panel (PD-L1, PD1, CD68, CD3, Ki67, panCK)

Multiplex immunohistochemistry - PD-L1 / PD1 Multiplex IHC-IF Antibody Panel (PD-L1, PD1, CD68, CD3, Ki67, panCK) (AB269812)

Key facts

Reacts with

Target

Applications

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise