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PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (AB252192), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (AB252192), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (AB252192), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (AB252192), expandable thumbnail

Publications

Key facts

Reactive species
Human

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PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Key facts

Reactive species
Human

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

PD1 Antibody Panel ab252192 contains multiple trial-sized versions of anti-human antibody clones against PD1, specifically selected for high performance in IHC. This panel contains 5 recombinant rabbit and mouse monoclonal antibodies against human PD1. They are provided in trial sizes to allow you to easily evaluate which is the best PD1 antibody for your IHC research assay needs.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [CAL20] to PD1 (20 μL) ab237728

- Rabbit monoclonal [EPR4877(2)] to PD1 (20 μL) ab137132

- Mouse monoclonal [NAT105] to PD1 (20 μL) ab52587

- Rabbit monoclonal [NAT105-EPR21106] to PD1 - Chimeric (20 μL) ab216352

- Rabbit monoclonal [SP269] to PD1 (20 μL) ab227681


Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.


Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PD1 also known as Programmed Cell Death Protein 1 or PDCD1 is a transmembrane protein that plays a critical role in regulating immune responses. It has a mass of approximately 55 kDa. PD1 is expressed on the surface of T cells B cells and some myeloid cells. PD1’s expression increases upon activation of these immune cells assisting in maintaining peripheral tolerance. Researchers often use PD1 mouse models and chimeric antibodies to explore the function of PD1 for experimental purposes. Antibodies such as anti-PD1 such as EH12.2H7 help in blocking PD1 interaction to study its role further.

Biological function summary

PD1 serves as an inhibitory receptor acting as a checkpoint in the immune system. It becomes part of an immune-suppressive complex when it binds with its ligands PD-L1 or PD-L2 which are expressed on various cell types including some tumor cells. This interaction suppresses the proliferation of T cells and cytokine production contributing to immune homeostasis. By controlling T cell activity PD1 limits autoimmunity but can also reduce the immune system's capability to attack cancer cells.

Pathways

PD1 functions in the immune checkpoint pathway a critical regulatory circuit in immune regulation. The engagement of PD1 with its ligands initiates a cascade that inhibits the function and proliferation of T cells through downstream SHP-2 phosphatase activity. This pathway frequently involves other regulatory proteins like CTLA-4 and is an important mechanism by which the body modulates immune responses. Related pathways often intersect with those involving T cell receptor signaling and contribute to the overall modulation of immune activity.

Associated diseases and disorders

PD1 has a significant role in cancer and autoimmune disorders. PD1 expression can allow tumors to evade immune surveillance making PD1 a target for cancer therapies such as anti-PD1 antibodies which aim to block PD1 and restore T cell activity. The interaction of PD1 with cancer-related proteins like PD-L1 facilitates tumor immune evasion. In autoimmune disorders PD1’s regulation of immune balance can become dysregulated leading to persistent immune activation and tissue damage. Understanding PD1 and its interaction with proteins such as PD-L1 helps in developing therapeutic strategies for both cancer and autoimmune conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for PD1 using Anti-PD1 antibody [SP269] ab227681 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PD1 with Anti-PD1 antibody [NAT105] - Rabbit IgG (Chimeric) ab216352 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Positive staining on T cells of human tonsil germinal center (PMID: 20087161; PMID: 17640856) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use.

    Perform antigen retrieval using Universal HIER antigen retrieval reagents (10X) (Universal HIER antigen retrieval reagent (10X) ab208572).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD1 with Anti-PD1 antibody [EPR4877(2)] ab137132 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA antigen retrieval solution, and microwave treatment for 20 min at 20% power. Anti-Rabbit HRP polymer was used as secondary antibody. Opal tyramide amplification was performed using Opal 540 fluorophore. Counterstained with DAPI stain.

    Image scanned with Vectra 3.0 and analyzed via Inform software.

    This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PD1 Antibody Panel (EPR4877(2), SP269, CAL20, NAT105, NAT105-EPR21106) (ab252192)

    Formalin-fixed, paraffin-embedded human lymph node tissue stained for PD1 using Anti-PD1 antibody [CAL20] ab237728 at 0.125 μg/ml dilution in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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