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PI3K/AKT signalling pathway panel containing 4 antibodies against PI 3 Kinase p85 alpha, AKT1, AKT2, AKT3 and mTOR

- Provided as a sampler panel to allow you to easily evaluate each in your required applications. 


Images

Immunocytochemistry - PI3K/AKT signalling pathway panel (AB283852), expandable thumbnail
  • Immunoprecipitation - PI3K/AKT signalling pathway panel (AB283852), expandable thumbnail
  • Western blot - PI3K/AKT signalling pathway panel (AB283852), expandable thumbnail
  • Western blot - PI3K/AKT signalling pathway panel (AB283852), expandable thumbnail
  • Immunocytochemistry - PI3K/AKT signalling pathway panel (AB283852), expandable thumbnail

Publications

Key facts

Reactive species
Mouse, Human, Rat
Target
PIK3R1
(See target data below)

Target data

Function

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

Additional Targets

MTOR, AKT1

Alternative names

Recommended products

PI3K/AKT signalling pathway panel containing 4 antibodies against PI 3 Kinase p85 alpha, AKT1, AKT2, AKT3 and mTOR

- Provided as a sampler panel to allow you to easily evaluate each in your required applications. 

Key facts

Reactive species
Mouse, Human, Rat
Target
PIK3R1

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Product Specifications
PI3K/AKT signalling pathway panel (ab283852) is a panel of 5 antibodies specific to the PI3K/AKT signalling pathway, which regulates cell frowth, survival, metabolism and proliferation.
This panel contains 4 rabbit recombinant monoclonal antibodies and 1 rabbit polyclonal antibody to PI 3 Kinase p85 alpha, PI 3 Kinase p85 alpha (pY607), [AKT1 + AKT2 + AKT3], [AKT1 (pS473) + AKT2 (pS474) + AKT3 (pS472)] and mTOR in convenient trial sizes (20 µL) to allow for efficient and cost-effect testing of the antibodies simultaneously.

The individual antibodies included in this panel are:

  • Rabbit recombinant monoclonal anti-PI 3 Kinase p85 alpha antibody [EPR18702] (ab191606)
  • Rabbit polyclonal anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
  • Rabbit recombinant monoclonal anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab179463)
  • Rabbit recombinant monoclonal anti-AKT1 (pS473) + AKT2 (pS474) + AKT3 (pS472) antibody [EPR18853] (ab192623)
  • Rabbit recombinant monoclonal anti-mTOR antibody [EPR390(N)] (ab134903)

All of these component antibodies are available for purchase individually using their corresponding abIDs.

Quality and Validation
Abcams high quality validation processes ensure each component antibody is sensitive and specific to the target protein.
All antibodies in this panel have been validated in human, mouse and rat samples in a variety of applications, and some also have reactivity in other species.
For information on the species reactivity and application validation of each antibody within the panel, please consult the individual datasheet for each antibody. Suggested dilution factor and additional information on protocols can also be found on the antibody datasheets.
All antibodies in this panel have a minimum of 60 citations in peer-reviewed journals and are trusted by scientific community.

Related Products
Many of our antibody clones are also available as carrier-free formulations for easy conjugation to labels of your choice, or in pre-conjugated formats. Please search the clone name for alternative formulations and related products.


We recommend using ab283852 together with ab205718.

Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MTOR also known as mechanistic target of rapamycin is a protein kinase involved in regulating cell growth proliferation motility and survival. It has a molecular weight of approximately 289 kDa and is expressed widely across various tissues notably in areas of high cellular activity. mTOR is part of the PI3K/AKT signaling pathway where it functions as a central regulator. PI3 kinase p85 alpha is a regulatory subunit of phosphoinositide 3-kinases which plays an important role in the modulation of AKT signaling. AKT1 AKT2 and AKT3 are serine/threonine-specific protein kinases that play an important role in transmitting signals downstream of PI3K with each isoform having unique tissue-specific roles.

Biological function summary

MTOR functions as a core component of two distinct complexes: mTORC1 and mTORC2. mTORC1 regulates protein synthesis by modulating the phosphorylation of key translational regulators while mTORC2 is involved in the regulation of the actin cytoskeleton. The PI3 kinase p85 alpha a regulatory entity mediates signaling through its interaction with catalytic subunits impacting glucose metabolism and growth. AKT being a pivotal node in cellular processes modulates glucose uptake and cell proliferation and prevents apoptosis through its activity. Collectively these proteins act to integrate signals from growth factors and nutrients to enact biological responses critical for cellular homeostasis.

Pathways

MTOR PI3 kinase p85 alpha and AKT isoforms are important components of the PI3K/AKT/mTOR pathway. This pathway plays a significant role in cellular metabolism growth survival and angiogenesis. Within this context mTOR acts downstream of AKT to promote protein synthesis and cell growth. PI3K signaling initiates AKT activation which then phosphorylates various substrates including mTOR. Members of this pathway such as PTEN regulate the pathway's activity negatively modulating cellular responses and ensuring proper cell function and survival.

Associated diseases and disorders

The dysregulation of mTOR PI3K and AKT is associated with cancer and type 2 diabetes. These proteins when aberrantly activated can lead to uncontrolled cell proliferation and tumorigenesis with mTOR being a target for rapamycin an anti-cancer drug. In type 2 diabetes insulin signaling involving the PI3K/AKT pathway becomes impaired leading to improper glucose homeostasis. The intricate relations of these proteins within these diseases highlight their potential as targets for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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