Plasma Membrane Marker (Na/K ATPase, pan Cadherin, PMCA1) Antibody Sampler Panel - Human, Mouse

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plasma Membrane Marker (Na/K ATPase, pan Cadherin, PMCA1) Antibody Sampler Panel - Human, Mouse (AB254025)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PMCA1 with ab190355 at 1/500 dilution. A prediluted HRP Polymer for Rabbit IgG as secondary antibody. Counterstained with Hematoxylin. Negative control also shown.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Plasma Membrane Marker (Na/K ATPase, pan Cadherin, PMCA1) Antibody Sampler Panel - Human, Mouse (AB254025)

ICC/IF image of ab22744 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab22744, 5μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Plasma Membrane Marker (Na/K ATPase, pan Cadherin, PMCA1) Antibody Sampler Panel - Human, Mouse (AB254025)

T84 cells (human) cultured on 8-well chamber slides were washed once with ice-cold PBS then fixed with 4% paraformaldehyde for 30 min at 4°C. After fixation cells were permeabilized with 0.5% Triton X-100 for 5 min at room temperature and washed with PBS three times. Following blocking with 2% FCS in PBS for 1 hour at room temperature primary antibody staining was performed at 4°C overnight at 1/200 dilution. Cells were then incubated with protein fractions B12 and C5 at 5x dilutions in fresh media for 1 hour at 37°C. Cells were then fixed permeabilized and co-stained with fiber and sodium potassium ATPase. The nuclei were stained with DAPI using Vectachield mounting medium. Cells were visualized using Zeiss confocal microscopy LSM700.

Fiber molecules were found to be predominantly intracellularly following B12 treatment.

For full image see PubMed : 25723153.

Zhang B et al., PLoS One, 10, e0117976, 2015 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plasma Membrane Marker (Na/K ATPase, pan Cadherin, PMCA1) Antibody Sampler Panel - Human, Mouse (AB254025)

Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab76020 at a working dilution of 1 in 100. The secondary antibody used is a HRP conjugated goat anti-rabbit IgG (H+L), ab97051, at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plasma Membrane Marker (Na/K ATPase, pan Cadherin, PMCA1) Antibody Sampler Panel - Human, Mouse (AB254025)

Immunohistochemical staining of paraffin embedded mouse liver with purified ab76020 at a working dilution of 1 in 100. The secondary antibody used is a HRP conjugated goat anti-rabbit IgG (H+L) ab97051 at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Plasma Membrane Marker (Na/K ATPase, pan Cadherin, PMCA1) Antibody Sampler Panel - Human, Mouse (AB254025)

IHC image of ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7671, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.