Protease and Phosphatase Inhibitor Cocktail

Notes

To protect against both protease and phosphatase in one solution try our dual cocktail (EDTA-free also available).

AEBSF free and mass spectroscopy compatible – water soluble.

Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, metalloproteases

Target phosphatases - serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases

Inhibitors included - aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate, EDTA disodium salt.

Form: Supplied as 1 vial of cocktail in water (1 ml) and 1 vial of EDTA disodium salt (0.5 mM in 1 ml of water)

Preparation: The recommended starting dilution is 1:9

Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.

This product is manufactured by BioVision, an Abcam company and was previously called K283 EZBlock™ Universal Protease and Phosphatase Inhibitor Cocktail. K283-1 is the same size as the 1 kit size of ab201119.

Individual components and concentration:

Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
E-64: cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
β-Glycerophosphate: serine/threonine phosphatase inhibitor (10 mM)
Sodium fluoride: acid phosphatase and serine/threonine phosphatase inhibitor (200 mM)
Sodium orthovanadate: protein tyrosine phosphatase and alkaline phosphatase inhibitor (1 mM)
Sodium pyrophosphate decahydrate: serine/threonine phosphatase inhibitor (10 mM)
EDTA disodium salt: metalloprotease inhibitor (0.5 mM)

Product promise