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AB201111

Protease Inhibitor Cocktail (EDTA-free)

5

(1 Review)

|

(30 Publications)

Protease inhibitor cocktail (ab201111) inhibit a variety of endogenous proteases to prevent protein sample degradation.

- Ideal for mammalian cell/tissue lysates and bacterial cell extracts.

Key facts

Product details

Protease Inhibitor Cocktail (EDTA-free) ab201111 inhibit a variety of endogenous proteases to protect your protein samples from degradation.

Ideal for mammalian cell/tissue lysates and bacterial cell extracts using metal chelation chromatography.

Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, aspartic proteases

Inhibitors included - AEBSF hydrochloride, aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, pepstatin A

Form: Supplied in DMSO (1 ml)

Preparation: The recommended starting dilution is 1:200

Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.

Individual components and concentrations of ab201111:

AEBSF hydrochloride: serine protease inhibitor (100 mM)
Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
E-64: cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
Pepstatin A: aspartic protease inhibitor (1 mM)

This product is manufactured by BioVision, an Abcam company and was previously called K272 EZBlock™ Protease Inhibitor Cocktail, EDTA-Free. K272-1 is the same size as the 1 ml size of ab201111.

This product is manufactured by BioVision, an Abcam company and was previously called K272 EZBlock™ Protease Inhibitor Cocktail, EDTA-Free. K272-1 is the same size as the 1 ml size of ab201111.

Individual components and concentrations:

AEBSF hydrochloride: serine protease inhibitor (100 mM)
Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
E-64: cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
Pepstatin A: aspartic protease inhibitor (1 mM)

What's included?

{ "values": { "1mL": { "sellingSize": "1 mL", "publicAssetCode":"ab201111-1mL", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Protease Inhibitor Cocktail (EDTA-free)", "number":"AB201111-CMP01", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 29:245 PubMed40182609

2025

The feasible role of soluble E‑cadherin in spheroidogenesis of HCT116 colorectal cancer cells, a candidate biomarker for liquid biopsy.

Applications

Unspecified application

Species

Unspecified reactive species

In-Youb Chang,Hye-Jin Boo,Jin Won Hyun,Sang-Pil Yoon

Physiological reports 13:e70222 PubMed39903552

2025

Improved glucose handling in female rat offspring of a hypertensive pregnancy with intrauterine growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa A Cedars,Kate M Root,Brian Akhaphong,Megan Beetch,Abigail E Miles,Ronald R Regal,Emilyn U Alejandro,Jean F Regal

Molecular therapy. Methods & clinical development 33:101390 PubMed39897642

2025

Silencing hepatic PCSK9 via novel chimeric AAV8 mitigates the progression of atherosclerosis by inhibiting inflammation in ApoE mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaocui Chen,Minghui Sun,Xiang Ma,Yitong Ma,Bangdang Chen

Microbial cell (Graz, Austria) 11:155-186 PubMed38783922

2024

Polyadenylated versions of small non-coding RNAs in are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome.

Applications

Unspecified application

Species

Unspecified reactive species

Anusha Chaudhuri,Soumita Paul,Mayukh Banerjea,Biswadip Das

Redox biology 73:103184 PubMed38718533

2024

Sirt5 improves cardiomyocytes fatty acid metabolism and ameliorates cardiac lipotoxicity in diabetic cardiomyopathy via CPT2 de-succinylation.

Applications

Unspecified application

Species

Unspecified reactive species

Maoxiong Wu,Jing Tan,Zhengyu Cao,Yangwei Cai,Zhaoqi Huang,Zhiteng Chen,Wanbing He,Xiao Liu,Yuan Jiang,Qingyuan Gao,Bingqing Deng,Jingfeng Wang,Woliang Yuan,Haifeng Zhang,Yangxin Chen

iScience 27:109523 PubMed38577103

2024

Cystine-cored diphenylalanine appended peptide-based self-assembled fluorescent nanostructures direct redox-responsive drug delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Suman Nayak,Kiran Das,Subramaniyam Sivagnanam,Shyamvarnan Baskar,Adele Stewart,Dinesh Kumar,Biswanath Maity,Priyadip Das

Journal of cellular and molecular medicine 28:e18246 PubMed38520223

2024

Intracerebroventricular BDNF infusion may reduce cerebral ischemia/reperfusion injury by promoting autophagy and suppressing apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Umit Yilmaz,Kevser Tanbek,Semir Gul,Ahmet Koc,Mehmet Gul,Suleyman Sandal

The FEBS journal 291:897-926 PubMed37994298

2023

RRM1 and PAB domains of translation initiation factor eIF4G (Tif4631p) play a crucial role in the nuclear degradation of export-defective mRNAs in Saccharomyces cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Upasana Saha,Rajlaxmi Gaine,Sunirmal Paira,Satarupa Das,Biswadip Das

Journal of dairy science 107:1197-1210 PubMed37709028

2023

Peripheral blood mononuclear cell mitochondrial enzyme activity in calves is associated with average daily gain, reproductive outcomes, lactation performance, and survival.

Applications

Unspecified application

Species

Unspecified reactive species

A M Niesen,H A Rossow

Neuroendocrinology 113:1035-1050 PubMed37321200

2023

Melatonin Attenuates Cerebral Ischemia/Reperfusion Injury through Inducing Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Umit Yilmaz,Kevser Tanbek,Semir Gul,Mehmet Gul,Ahmet Koc,Suleyman Sandal
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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