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Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

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Key facts

Reactive species

Human

Target

CD4

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Target data

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.(Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:2214026, PubMed:16331979, PubMed:9641677, PubMed:12089508). Down-regulated by HIV-1 Vpu (PubMed:17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607).

Alternative names

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Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.

Alternative names

Key facts

Reactive species

Human

Target

CD4

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human ab254021 contains multiple trial-sized versions of anti-human antibody clones against CD4, FOXP3, CTLA4, CCR6, HLA-DR, specifically selected for high performance in various applications. This panel contains 5 recombinant rabbit monoclonal antibodies against human CD4, FOXP3, CTLA4, CCR6 and 1 mouse monoclonal antibody against HLA-DR. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.

For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.

Panel contains:

- Rabbit monoclonal [EPR6855] to CD4 (20 μL) ab133616

- Rabbit monoclonal [EPR22102-37] to FOXP3 (20 μL) ab215206

- Rabbit monoclonal [CAL49] to CTLA4 (20 μL) ab237712

- Rabbit monoclonal [EPR22259] to CCR6 (20 μL) ab227036

- Mouse monoclonal [TAL 1B5] to HLA-DR (20 μg) ab20181


Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.


Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD4 with purified ab133616 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021)

    Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CCR6 with ab227036 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human Hodgkin's lymphoma (PMID: 12514792) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling FOXP3 with ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in human thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CTLA4 using ab237712 at 0.25 μg/ml in immunohistochemical analysis.

    Incubate with primary antibody for 75 minutes at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Regulatory T Cell Marker (CD4, FOXP3, CTLA4, CCR6, HLA-DR) Antibody Panel - Human (ab254021)

    Anti-CD4 antibody [EPR6855] (ab133616)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD4 with ab133616 at a dilution of 1:500. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
    This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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