Stimulatory Immune Checkpoint Panel - Human IHC (ab278178) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Images
Key facts
- Reactive species
Human
- Target
CD27
(See target data below)
Target data
Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Targets
CD226, CD40, ICOS, TNFRSF18, STING1, CD28, TNFRSF4
Alternative names
CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7
What's included?
Recommended products
Stimulatory Immune Checkpoint Panel - Human IHC (ab278178) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Alternative names
CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7
Key facts
- Reactive species
Human
- Target
CD27
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Stimulatory Immune Checkpoint Panel - Human IHC ab278178 contains multiple trial-sized versions of anti-human antibody clones against CD134 / OX40L, ICOS, CD40, CD28, CD27, CD226, STING/TMEM173 and GITR specifically selected for high performance in various applications. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EPR23000-42] to CD134 / OX40L receptor (20 μL) ab264466
- Rabbit monoclonal [EPR20560] to ICOS (20 μL) ab224644
- Rabbit monoclonal [EPR20735] to CD40 (20 μL) ab224639
- Rabbit monoclonal [EPR22076] to CD28 (20 μL) ab243228
- Rabbit monoclonal [EPR8569] to CD27 (20 μL) ab131254
- Rabbit monoclonal [EPR21172] to CD226 (20 μL) ab212076
- Rabbit monoclonal [EPR13130-55] to STING/TMEM173 (20 μL) ab239074
- Rabbit monoclonal [CAL8] to GITR (20 μL) ab237714
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
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Full details and terms and conditions can be found here:
Terms & Conditions.
19 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling GITR with Anti-GITR antibody [CAL8] ab237714 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human tonsil tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for TNFSF18 using Anti-GITR antibody [CAL8] ab237714 at 0.5 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD40 with Anti-CD40 antibody [EPR20735] ab224639 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human tonsil tissue (PMID:20593977; PMID:7507299) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling TMEM173 with Anti-STING antibody [EPR13130-55] ab239074 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in human colon cancer (PMID: 26748708) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling TMEM173 with Anti-STING antibody [EPR13130-55] ab239074 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in human tonsil (PMID:28874664) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue labeling TMEM173 with Anti-STING antibody [EPR13130-55] ab239074 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in basal cells of human prostate hyperplasia is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CD134 / OX40L receptor with Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human Hodgkin's lymphoma (PMID/8547142). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human bone marrow tissue labeling CD226 with Anti-CD226 antibody [EPR21172] ab212076 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in megakaryocytes of human bone marrow (PMID: 15693793) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Perform heat-induced antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD226 with Anti-CD226 antibody [EPR21172] ab212076 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining of platelets and endothelium in human spleen is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Perform heat-induced antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling CD134 / OX40L with Anti-CD134 / OX40L receptor antibody [EPR23000-42] ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on T cells of human thymus (PMID/8547142). Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human stomach tissue labelling CD27 with Anti-CD27 antibody [EPR8569] ab131254 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD27 with purified Anti-CD27 antibody [EPR8569] ab131254 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody.
Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human tonsil tissue labelling CD27 with Anti-CD27 antibody [EPR8569] ab131254 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue showing negative staining with purified Anti-CD27 antibody [EPR8569] ab131254 at 1/800.
Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody.
Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling CD40 with Anti-CD40 antibody [EPR20735] ab224639 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining in human diffuse large B-cell lymphoma tissue (PMID: 20593977) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CD40 with Anti-CD40 antibody [EPR20735] ab224639 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in Reed-Sternberg cells and tumor cells of human Hodgkin's lymphoma (PMID:7507299) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling ICOS with Anti-ICOS antibody [EPR20560] ab224644 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in tumor infiltrating lymphoid cells of human cervix cancer (PMID: 23802069) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling ICOS with Anti-ICOS antibody [EPR20560] ab224644 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in a population of lymphoid cells of human stomach (magnification x200) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ICOS with Anti-ICOS antibody [EPR20560] ab224644 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in a population of lymphoid cells of human tonsil (PMID: 20207847), (magnification x200) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
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