Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel

• IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (AB226492)

IHC image of Tau (phospho T231) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with ab151559 at 1/2000 dilution for 2 hours at 21°C. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.

Image courtesy of Carl Hobbs, Kings College London.

• IHC-P

AbReview39770****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (AB226492)

IHC image of Tau (phospho S396) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with unpurified ab109390 at 1/1000 dilution for 2 hoursat 21°C. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.

Image courtesy of Carl Hobbs, Kings College London, U.K.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (AB226492)

ICC/IF image of ab80579 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80579, 10μg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• Dot

Unknown

Dot Blot - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (AB226492)

Dot blot analysis of Tau (pS199) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS199) with ab81268 at a dilution of 1/1000. ab97051 (peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Exposure time : 3 minutes.
Blocking and dilution buffer : 5% NFDM/TBST.

• Dot

Unknown

Dot Blot - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (AB226492)

Dot blot analysis of Tau (pS422) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS422) with ab79415 at a dilution of 1/1000. ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

• Dot

Supplier Data

Dot Blot - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (AB226492)

Dot blot analysis of Tau (phospho S202+ T205) labeled with ab210703 at 1/1000 dilution.

Lane 1 : Tau (phospho S202 + T205) peptide.
Lane 2 : Tau (phospho S202) peptide.
Lane 3 : Tau (phospho T205) peptide.
Lane 4 : Tau non-phospho peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.