Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (ab226492) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (ab226492) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
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- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
ab226492 is a tau research panel containing 1 recombinant mouse monoclonal antibody, 1 anti-mouse secondary antibody, 8 recombinant rabbit monoclonal antibodies and 1 anti-rabbit secondary antibody: Anti-Tau antibody, Goat Anti-Mouse IgG (H&L) (HRP), Anti-Tau (phospho S396) antibody, Anti-Tau (phospho T231) antibody, Anti-Tau (phospho S422) antibody, Anti-Tau (phospho S199) antibody, Anti-Tau (phospho S404) antibody, Anti-Tau (phospho S198) antibody, Anti-Tau (phospho S214) antibody, Anti-Tau (phospho S202 + T205) antibody, and Goat Anti-Rabbit IgG (H&L) (HRP).
Under normal circumstances, tau is a microtubule-associated protein involved in microtubule stabilization, but under pathological conditions, tau becomes hyperphosphorylated and detaches from microtubules. Phosphorylated tau then aggregates to form paired helical filaments (PHFs) and neurofibrillary tangles (NFTs).
The antibodies in this panel were selected for their exceptional performance. Please see the individual datasheets for additional information.
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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6 product images
Image courtesy of Carl Hobbs, Kings College London, U.K.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (ab226492)
IHC image of Tau (phospho S396) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with unpurified Anti-Tau (phospho S396) antibody [EPR2731] ab109390 at 1/1000 dilution for 2 hoursat 21°C. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.
Immunocytochemistry/ Immunofluorescence - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (ab226492)
ICC/IF image of Anti-Tau antibody [TAU-5] - BSA and Azide free ab80579 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (Normal Goat Serum ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tau antibody [TAU-5] - BSA and Azide free ab80579, 10μg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Image courtesy of Carl Hobbs, Kings College London.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (ab226492)
IHC image of Tau (phospho T231) staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with citrate buffer. The section was then incubated with Anti-Tau (phospho T231) antibody [EPR2488] ab151559 at 1/2000 dilution for 2 hours at 21°C. A biotin conjugated goat-anti-rabbit antibody was used as a secondary at 1/250. The section shows clear neurofibrillary tangles in a subset of neurons.
Dot Blot - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (ab226492)
Dot blot analysis of Tau (pS422) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS422) with Anti-Tau (phospho S422) antibody [EPR2866] ab79415 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Dot Blot - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (ab226492)
Dot blot analysis of Tau (phospho S202+ T205) labeled with Anti-Tau (phospho S202 + T205) antibody [EPR20390] ab210703 at 1/1000 dilution.
Lane 1: Tau (phospho S202 + T205) peptide.
Lane 2: Tau (phospho S202) peptide.
Lane 3: Tau (phospho T205) peptide.
Lane 4: Tau non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Dot Blot - Tau Research (Tau, S198, S199, S202 + T205, S214, S396, S404, S422, T231) Antibody Sampler Panel (ab226492)
Dot blot analysis of Tau (pS199) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS199) with Anti-Tau (phospho S199) antibody [EPR2401Y] ab81268 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Exposure time: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.
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