Wnt/Beta Catenin Signaling Pathway Antibody Sampler Panel

5 product images

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  Immunocytochemistry/ Immunofluorescence - Wnt/Beta Catenin Signaling Pathway Antibody Sampler Panel (ab228526)

  LEF1 immunofluorescence staining of Jurkat cells using rabbit anti-LEF1 antibody
  
  Immunofluorescence staining of Jurkat (Human T cell leukemia cell line from peripheral blood) cells with purified Anti-LEF1 antibody [EPR2029Y] ab137872 at a working dilution of 1 in 500 counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and Alexa Fluor^® 594 goat anti-mouse at a dilution of 1/500 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) . The secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Alexa Fluor^® 488 goat anti rabbit used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100.

  The negative controls are shown in the bottom middle and right hand panels - for the first negative control purified Anti-LEF1 antibody [EPR2029Y] ab137872 was used at a dilution of 1/200 followed by an Alexa Fluor^® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (ab15007) were used.

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  This image is courtesy of a customer review submitted by David Ivancic.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt/Beta Catenin Signaling Pathway Antibody Sampler Panel (ab228526)

  Anti-Met (c-Met) antibody [EP1454Y] - N-terminal ab51067 staining Met (c-Met) in human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% tween 20 and blocked for 30 minutes at 22°C; antigen retrieval was by heat mediation in Antigen Retrieval Buffer (100X Citrate Buffer pH 6.0) (ab94674). Samples were incubated with the primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt/Beta Catenin Signaling Pathway Antibody Sampler Panel (ab228526)

  Immunohistochemical analysis of paraffin-embedded human mantle cell lymphoma tissue, labeling Cyclin D1 with unpurified Anti-Cyclin D1 antibody [EPR2241] - C-terminal ab134175 at 1/100 dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt/Beta Catenin Signaling Pathway Antibody Sampler Panel (ab228526)

  IHC image of Anti-c-Myc antibody [Y69] - ChIP Grade ab32072 staining c-Myc in human esophagus formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-c-Myc antibody [Y69] - ChIP Grade ab32072, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the Secondary only control (shown on the inset).
  

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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  Immunocytochemistry/ Immunofluorescence - Wnt/Beta Catenin Signaling Pathway Antibody Sampler Panel (ab228526)

  Anti-c-Jun antibody [E254] - ChIP Grade ab32137 staining c-Jun in HeLa cells treated with curcumin (diferuloylmethane) (Curcumin (Diferuloylmethane), Lipooxygenase inhibitor ab120618), by ICC/IF. Decrease in c-Jun expression correlates with increased concentration of curcumin (diferuloylmethane) as described in literature.
  The cells were incubated at 37°C for 4h in media containing different concentrations of Curcumin (Diferuloylmethane), Lipooxygenase inhibitor ab120618 (curcumin (diferuloylmethane)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-c-Jun antibody [E254] - ChIP Grade ab32137 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.