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AB273327

15-PGDH Activity Assay Kit (Fluorometric)

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15-PGDH Activity Assay Kit (Fluorometric) (ab273327) offers a rapid, simple, sensitive, and reliable test suitable for detecting as low as 1 µU of 15-Hydroxy prostaglandin dehydrogenase (15-PGDH).

- Includes PGDH positive control, and standard for quantitation
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

PGDH1, SDR36C1, HPGD, 15-hydroxyprostaglandin dehydrogenase [NAD(+)], 15-PGDH, Eicosanoid/docosanoid dehydrogenase [NAD(+)], Prostaglandin dehydrogenase 1, Short chain dehydrogenase/reductase family 36C member 1

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Functional Studies - 15-PGDH Activity Assay Kit (Fluorometric) (AB273327)
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Supplier Data

Functional Studies - 15-PGDH Activity Assay Kit (Fluorometric) (AB273327)

Kinetic measurement of 15-PGDH Specific Activity in lysates prepared from mouse liver (50 μg) and 15-PGDH Positive Control.

Functional Studies - 15-PGDH Activity Assay Kit (Fluorometric) (AB273327)
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Supplier Data

Functional Studies - 15-PGDH Activity Assay Kit (Fluorometric) (AB273327)

NADH standard curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Functional Studies - 15-PGDH Activity Assay Kit (Fluorometric) (AB273327)
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Supplier Data

Functional Studies - 15-PGDH Activity Assay Kit (Fluorometric) (AB273327)

15-PGDH specific activity of mouse lysates from liver, kidney and lung.

15-PGDH specific activity of mouse lysates from liver (50 μg), kidney (16.5 μg) and lung (41 μg). Assays were performed following the kit protocol.

Key facts

Detection method

Fluorescent

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Enzyme activity (quantitative)

Sensitivity

= 1 µU

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

15-PGDH Activity Assay Kit (Fluorometric) (ab273327) offers a rapid, simple, sensitive, and reliable test suitable for detecting as low as 1 μU of 15-Hydroxy prostaglandin dehydrogenase (15-PGDH; EC:1.1.1.141) activity.

In the assay, 15-PGDH oxidizes a substrate forming intermediates and NADH. The oxidation of NADH reduces a probe generating fluorescence at Ex/Em=535/587 nm. The activity of 15-PGDH is proportional to the fluorescent signal.

This product is manufactured by BioVision, an Abcam company and was previously called K562 PicoProbe™ 15-PGDH Activity Assay Kit (Fluorometric). K562-100 is the same size as the 100 test size of ab273327.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273327-100Test", "assetComponentDetails": [ { "size":"1 x 0.4 mL", "name":"PicoProbe I", "number":"AB273327-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution X", "number":"AB273327-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADH Standard II", "number":"AB273327-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"15-PGDH Positive Control", "number":"AB273327-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"15-PGDH Assay Buffer", "number":"AB273327-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"15-PGDH Substrate", "number":"AB273327-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

15-PGDH also known as 15-hydroxyprostaglandin dehydrogenase PGDH or 15-PGDH protein is an enzyme with a molecular mass of approximately 29 kDa. This enzyme catalyzes the oxidation of the 15-hydroxyl group in prostaglandins specifically converting Prostaglandin E2 (PGE2) into its inactive form. Expression occurs in various tissues including the lung placenta and colonic epithelium. Its activity levels can vary depending on the tissue context and physiological conditions marking its importance in local regulation.
Biological function summary

15-PGDH regulates prostaglandin levels which are lipid compounds playing roles in inflammation and homeostasis. This enzyme does not associate with protein complexes but serves a standalone function in metabolic pathways. It maintains a balance between prostaglandin synthesis and degradation ensuring that inflammatory responses do not exceed what is necessary. The enzyme's activity directly reduces available prostaglandins which in turn dampens related signaling pathways.

Pathways

Prostaglandins and this enzyme are part of arachidonic acid metabolism and the Cyclooxygenase (COX) pathway. These pathways play key roles in inflammation and tissue homeostasis. 15-PGDH interacts with other enzymes like COX-2 as it acts downstream to mitigate PGE2 levels generated by COX enzymes. This diminishes the signaling of other elements such as the AMPA receptors involved in neuronal plasticity and pain perception showcasing the enzyme's regulatory scope within biological systems.

15-PGDH plays a role in colon cancer and pulmonary inflammation. Low expression or activity of this enzyme is associated with increased PGE2 levels contributing to cancer progression and chronic inflammation. This dysregulation may involve proteins like COX-2 where the imbalance between synthesis and degradation leads to elevated prostaglandin signaling. Understanding 15-PGDH functions offers insights into therapeutic potentials making inhibitors and potentiators of interest for treatment of these conditions.
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Product protocols

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Target data

Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites (PubMed : 10837478, PubMed : 16757471, PubMed : 16828555, PubMed : 21916491, PubMed : 25586183, PubMed : 8086429). Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression (PubMed : 15574495, PubMed : 25586183). Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation (PubMed : 16757471, PubMed : 22844113).
See full target information HPGD
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