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AB235976

2-NBDG Glucose Uptake Assay Kit

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(19 Publications)

2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake in cell cultures.

- includes glucose uptake inhibitor, and also propidium iodide to exclude dead cells
- use with standard fluorometric plate reader, fluorescent microscope or flow cytometer; Ex/Em 485/535 nm
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Functional Studies - 2-NBDG Glucose Uptake Assay Kit (AB235976)
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Supplier Data

Functional Studies - 2-NBDG Glucose Uptake Assay Kit (AB235976)

NBD-glucose is taken up by Jurkat cells in a dose-dependent manner.

Jurkat (Human T cell leukemia cell line from peripheral blood) cells were equilibrated in glucose-free medium for two hours prior to being treated with the indicated dilution of NBD-glucose for 10 minutes at 37°C. Cells were washed and data were collected on a MACSQuant cytometer. NBD-glucose fluorescence within the live population is shown.

Key facts

Detection method

Fluorescent

Sample types

Adherent cells, Suspension cells

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Microplate reader, Fluor. microscope, Flow cyt.

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Product details

2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake. The kit uses 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells.

The 2-NBDG assay kit is suitable for analysis with flow cytometer, fluorescent microscope or microplate reader.

2-NBDG protocol summary:
- treat cells with experimental treatment in glucose-free medium
- add 2-NBDG and incubate for 10 min
- remove cell culture supernatant and wash cells with cell-based assay buffer
- add propidium iodide at this point to exclude dead cells if analyzing with flow cytometry
- add cell-based assay buffer and analyze immediately
2-NBDG can be detected with filters designed to detect FITC (Ex/Em 485/535 nm).

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235976-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Tablet", "name":"Cell-Based Assay Buffer Tablet", "number":"AB235976-CMP02", "productcode":"" }, { "size":"1 x 500 µL", "name":"Cell-Based Assay NBD Glucose", "number":"AB235976-CMP03", "productcode":"" }, { "size":"1 x 250 µL", "name":"Cell-Based Propidium Iodide Solution", "number":"AB235976-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information 2-NBDG
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40277886

2025

Nicotinamide Inhibits CD4+ T-Cell Activation and Function.

Applications

Unspecified application

Species

Unspecified reactive species

Lotte Nijhuis,Alejandra Bodelόn,Rianne C Scholman,Isabelle Houtzager,Lyanne J P M Sijbers,Enric Mocholi,Lucas W Picavet,Jorg J A Calis,Michal Mokry,Sebastiaan J Vastert,Jorg van Loosdregt

Theranostics 15:3055-3075 PubMed40083924

2025

Targeting platinum-resistant ovarian cancer by disrupting histone and RAD51 lactylation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenggong Sun,Xiao Li,Qiuli Teng,Xihan Liu,Li Song,Helgi B Schiöth,Huan Wu,Xinyue Ma,Zhaoyang Zhang,Changjian Qi,Haocheng Zhang,Kun Song,Qing Zhang,Beihua Kong

Cell death discovery 11:47 PubMed39910045

2025

TMEM45A enhances palbociclib resistance and cellular glycolysis by activating AKT/mTOR signaling pathway in HR+ breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cui Chen,Zehong Chen,Jinze Zhao,Xinyun Wen,Hanming Yao,Zijin Weng,Huiping Xiong,Zongheng Zheng,Juekun Wu

Stem cell research & therapy 15:303 PubMed39278906

2024

Proteomics reveals dynamic metabolic changes in human hematopoietic stem progenitor cells from fetal to adulthood.

Applications

Unspecified application

Species

Unspecified reactive species

Mingfang Xiong,Yanyu Xiu,Juan Long,Xiao Zhao,Qianqian Wang,Haoyu Yang,Hang Yu,Lihong Bian,Yan Ju,Hongyu Yin,Qingxiang Hou,Fei Liang,Nan Liu,Fudong Chen,Ruiwen Fan,Yuying Sun,Yang Zeng

Heliyon 10:e32914 PubMed38994113

2024

Xihuang pills targeting the Warburg effect through inhibition of the Wnt/β-catenin pathway in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fengxia Lin,Yan Long,Mingyue Li,Changlong Cai,Yongrong Wu,Xujun You,Xuefei Tian,Qing Zhou

ACS applied materials & interfaces 16:29686-29698 PubMed38813771

2024

Gemcitabine-Lipid Conjugate and ONC201 Combination Therapy Effectively Treats Orthotopic Pancreatic Tumor-Bearing Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Prakash Shrestha,Yashwardhan Ghanwatkar,Sohan Mahto,Nilkamal Pramanik,Ram I Mahato

Science advances 10:eadi2012 PubMed38241371

2024

Tumor cell-intrinsic PD-1 promotes Merkel cell carcinoma growth by activating downstream mTOR-mitochondrial ROS signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Martins,Erik Rasbach,Markus V Heppt,Praveen Singh,Zsofi Kulcsar,Julia Holzgruber,Asmi Chakraborty,Kyla Mucciarone,Sonja Kleffel,Anne Brandenburg,Wolfram Hoetzenecker,Nuh N Rahbari,James A DeCaprio,Manisha Thakuria,George F Murphy,Matthew R Ramsey,Christian Posch,Steven R Barthel,Tobias Schatton

Cell death discovery 9:127 PubMed37059726

2023

Sestrin2-mediated disassembly of stress granules dampens aerobic glycolysis to overcome glucose starvation.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Li,Rick Francis Thorne,Ruijie Wang,Leixi Cao,Fangyuan Cheng,Xuedan Sun,Mian Wu,Jianli Ma,Lianxin Liu

Journal of Cancer 14:943-951 PubMed37151384

2023

Phosphofructokinase Platelet Overexpression Accelerated Colorectal Cancer Cell Growth and Motility.

Applications

Unspecified application

Species

Unspecified reactive species

Tzung-Ju Lu,Yi-Fen Yang,Ching-Feng Cheng,Ya-Ting Tu,Yi-Ru Chen,Ming-Cheng Lee,Kuo-Wang Tsai

Cell death & disease 14:82 PubMed36737598

2023

Metformin induces pyroptosis in leptin receptor-defective hepatocytes via overactivation of the AMPK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bingli Liu,Jingyuan Xu,Linyao Lu,Lili Gao,Shengjuan Zhu,Yi Sui,Ting Cao,Tao Yang
View all publications
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