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2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake in cell cultures.

- includes glucose uptake inhibitor, and also propidium iodide to exclude dead cells
- use with standard fluorometric plate reader, fluorescent microscope or flow cytometer; Ex/Em 485/535 nm

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Images

Functional Studies - 2-NBDG Glucose Uptake Assay Kit (AB235976), expandable thumbnail

Publications

Key facts

Detection method
Fluorescent
Sample types
Adherent cells, Suspension cells
Assay type
Cell-based (quantitative)

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Target data

What's included?

1 x 96 Tests
Components
Cell-Based Assay Apigenin
1 x 100 µL
Cell-Based Assay Buffer Tablet
1 x 1 Tablet
Cell-Based Assay NBD Glucose
1 x 500 µL
Cell-Based Propidium Iodide Solution
1 x 250 µL

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2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake in cell cultures.

- includes glucose uptake inhibitor, and also propidium iodide to exclude dead cells
- use with standard fluorometric plate reader, fluorescent microscope or flow cytometer; Ex/Em 485/535 nm

Key facts

Detection method
Fluorescent
Sample types
Adherent cells, Suspension cells
Assay type
Cell-based (quantitative)
Assay Platform
Microplate reader, Fluor. microscope, Flow cyt.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake. The kit uses 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells.

The 2-NBDG assay kit is suitable for analysis with flow cytometer, fluorescent microscope or microplate reader.

2-NBDG protocol summary:
- treat cells with experimental treatment in glucose-free medium
- add 2-NBDG and incubate for 10 min
- remove cell culture supernatant and wash cells with cell-based assay buffer
- add propidium iodide at this point to exclude dead cells if analyzing with flow cytometry
- add cell-based assay buffer and analyze immediately
2-NBDG can be detected with filters designed to detect FITC (Ex/Em 485/535 nm).

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1 product image

  • Functional Studies - 2-NBDG Glucose Uptake Assay Kit (ab235976), expandable thumbnail

    Functional Studies - 2-NBDG Glucose Uptake Assay Kit (ab235976)

    NBD-glucose is taken up by Jurkat cells in a dose-dependent manner.

    Jurkat (Human T cell leukemia cell line from peripheral blood) cells were equilibrated in glucose-free medium for two hours prior to being treated with the indicated dilution of NBD-glucose for 10 minutes at 37°C. Cells were washed and data were collected on a MACSQuant cytometer. NBD-glucose fluorescence within the live population is shown.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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